Cited by 94Bacteriophages and microbial interactionsEnzyme Structure and FunctionBacterial Genetics and Biotechnology

Modular Organization of T4 DNA Polymerase

Open access
Wang, Chien-Chia, Yeh, Lu-Shu, Karam, Jim D.·1995·Journal of Biological Chemistry

Abstract

We describe the use of a phylogenetic approach to analyze the modular organization of the single-chained (898 amino acids) and multifunctional DNA polymerase of phage T4. We have identified, cloned in expression vectors, and sequenced the DNA polymerase gene (gene 43) of phage RB69, a distant relative of T4. The deduced primary structure of the RB69 protein (RB69 gp43) differs from that of T4 gp43 in discrete clusters of short sequence that are interspersed with clusters of high similarity between the two proteins. Despite these differences, the two enzymes can substitute for each other in phage DNA replication, although T4 gp43 does exhibit preference to its own genome. A 55-amino acid internal gp43 segment of high sequence divergence between T4 and RB69 could be replaced in RB69 gp43 with the corresponding segment from T4 without loss of replication function. The reciprocal chimera and a deletion mutant of the T4 gp43 segment were both inactive for replication and specifically inhibitory ("dominant lethal") to the T4 wild-type allele. The results show that phylogenetic markers can be used to construct chimeric and truncated froms of gp43 that, although inactive for replication, can still exhibit biological specificity.

Integrity signals

Registry

Crossref · 10.1074/jbc.270.44.26558

Cites

33 of 33 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • PI3 kinase inhibitor wortmannin inhibits insulin but not contraction-stimulated glucose uptake.

    “1) PI3 kinase inhibitor wortmannin inhibits insulin but not contraction-stimulated glucose uptake. Contraction does not promote IRS-1 autophosphorylation or increase PI3 kinase activity [103-106].”

    From AMPK and Exercise: Glucose Uptake and Insulin Sensitivity (2013) · cites this paper as [103]

  • Contraction does not promote IRS-1 autophosphorylation or increase PI3 kinase activity.

    “1) PI3 kinase inhibitor wortmannin inhibits insulin but not contraction-stimulated glucose uptake. Contraction does not promote IRS-1 autophosphorylation or increase PI3 kinase activity [103-106].”

    From AMPK and Exercise: Glucose Uptake and Insulin Sensitivity (2013) · cites this paper as [103]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Wang, C.-C., Yeh, L.-S., & Karam, J. D.
Affiliations
Tulane University (US)
Corresponding author
Karam, Jim D.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1995-11
Volume
270
Issue
44
Pages
26558-26564
ISSN
0021-9258 (ISSN-L)
OpenAlex
W1970574123
Cited by
94
References
33
Citations by year
2025: 2 · 2023: 1 · 2022: 1 · 2020: 1 · 2014: 2 · 2013: 3
FWCI
10.92
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r01gm018842-20)
  • National Institute of General Medical Sciences (GM 18842)
Topics
  • Physical Sciences › Environmental Science › Ecology › Bacteriophages and microbial interactions
  • Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
T4 DNA polymerase, protein domain organization, fusion protein, dominant lethals, sequence divergence, phage DNA replication, phylogenetic analysis, deletion mutants
MeSH terms
Amino Acid Sequence, Base Sequence, DNA-Directed DNA Polymerase, DNA, Viral, Genes, Viral, Molecular Sequence Data, Phylogeny, Recombinant Proteins, Sequence Homology, Nucleic Acid, Genetic Variation, Viral Proteins, Blotting, Southern, Polymerase Chain Reaction, Bacteriophage T4, Sequence Homology, Amino Acid

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review