Cited by 105Cellular transport and secretionProtein Kinase Regulation and GTPase SignalingReceptor Mechanisms and Signaling

ADP-ribosylation Factor Functions Synergistically with a 50-kDa Cytosolic Factor in Cell-free Activation of Human Neutrophil Phospholipase D

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Lambeth, J. David, Kwak, Jong-Young, Bowman, Edward P., Perry, David, Uhlinger, David J., Lopez, Isabel·1995·Journal of Biological Chemistry

Abstract

Proteins in both the cytosol and plasma membrane are needed to reconstitute cell-free phospholipase D activity from phagocytes (Olson, S., Bowman, E. P., and Lambeth, J. D. (1991) J. Biol. Chem. 266, 17236-17242); membrane factors include a small GTP-binding protein in the Rho family (Bowman, E., Uhlinger, D. J., and Lambeth, J. D. (1993) J. Biol. Chem. 268, 21509-21512). ADP-ribosylation factor (ARF) was recently implicated as the cytosolic factor, as it activates phospholipase D in HL-60 membranes. Herein, we show that ion exchange chromatography separates ARF from the major phospholipase D-stimulating cytosolic factor. Both bovine brain ARF and recombinant human ARF-1 stimulated a small amount of phospholipase D activity in the absence of cytosol (about 10% of the response seen with cytosol). With a high concentration of ARF-depleted cytosol, ARF did not further activate. However, at low cytosol, ARF caused marked activation. Thus, ARF synergizes with the cytosolic factor in phospholipase D activation.

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Crossref · 10.1074/jbc.270.6.2431

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Authors
Lambeth, J. D., Kwak, J.-Y., Bowman, E. P., Perry, D., Uhlinger, D. J., & Lopez, I.
Affiliations
Emory University (US)
Corresponding author
Lambeth, J. David
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1995-02
Volume
270
Issue
6
Pages
2431-2434
ISSN
0021-9258 (ISSN-L)
OpenAlex
W2079169500
Cited by
104
References
31
Citations by year
2021: 1 · 2019: 1 · 2017: 1 · 2013: 1 · 2012: 1
FWCI
8.10
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r01ca046508-13)
  • National Cancer Institute (CA46508)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
Keywords
ADP-ribosylation factor, phospholipase D, neutrophils, enzyme activation, cytosolic factors, synergistic activation, Rho GTP-binding proteins, phagocytes, small GTPases
MeSH terms
Animals, Cattle, Cell-Free System, Cytosol, Enzyme Activation, Humans, Nerve Tissue Proteins, Neutrophils, Phospholipase D, Subcellular Fractions, GTP-Binding Proteins, ADP-Ribosylation Factors

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