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The Yeast Pan2 Protein Is Required for Poly(A)-binding Protein-stimulated Poly(A)-nuclease Activity
Open accessAbstract
The removal of the mRNA poly(A) tail in the yeast Saccharomyces cerevisiae is stimulated by the poly(A)-binding protein (Pab1p). A large scale purification of the Pab1p-stimulated poly(A) ribonuclease (PAN) identifies a 76-kDa and two 135-Da polypeptides as candidate enzyme subunits. Antibodies against the Pan1p protein, which is the minor 135-kDa protein in the preparation, can immunodeplete Pan1p but not PAN activity. The protein sequence of the major 135-kDa protein, Pan2p, reveals a novel protein that was also found in the previously reported PAN purification (Sachs, A. B., and Deardorff, J. A. (1992) Cell 70, 961-973). Deletion of the non-essential PAN2 gene results in an increase of the average length of mRNA poly(A) tails in vivo, and a loss of Pab1p-stimulated PAN activity in crude extracts. These data confirm that Pan2p and not Pan1p is required for PAN activity, and they suggest that ribonucleases other than the Pab1p-stimulated PAN are capable of shortening poly(A) tails in vivo.
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Citations
Cites
Messenger RNA degradation in eukaryotes
Sachs, A. B. (1993) · Cell · doi:10.1016/0092-8674(93)80043-e · Cited by 846
Self-citation
Cited by
Brennan, C. M., & Steitz*, J. A. (2001) · Cellular and Molecular Life Sciences · doi:10.1007/pl00000854 · Cited by 1,095
The cap-to-tail guide to mRNA turnover
Wilusz, C. J., Wormington, M., & Peltz, S. W. (2001) · Nature Reviews Molecular Cell Biology · doi:10.1038/35067025 · Cited by 759
Association of the yeast poly(A) tail binding protein with translation initiation factor eIF-4G.
Tarun, S. Z., & Sachs, A. B. (1996) · The EMBO Journal · doi:10.1002/j.1460-2075.1996.tb01108.x · Cited by 688
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Braun, J. E., Huntzinger, E., Fauser, M., & Izaurralde, E. (2011) · Molecular Cell · doi:10.1016/j.molcel.2011.09.007 · Cited by 377
Messenger RNA Turnover in Eukaryotes: Pathways and Enzymes
Meyer, S., Temme, C., & Wahle, E. (2004) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.1080/10409230490513991 · Cited by 342
Metadata
- Authors
- Boeck, R., Tarun, S., Rieger, M., Deardorff, J. A., Müller-Auer, S., & Sachs, A. B.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1996-01
- Volume
- 271
- Issue
- 1
- Pages
- 432-438
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2093633160
- Cited by
- 195
- References
- 1
- FWCI
- 4.33
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Boeck, R., Tarun, S., Rieger, M., Deardorff, J. A., Müller-Auer, S., & Sachs, A. B. (1996). The Yeast Pan2 Protein Is Required for Poly(A)-binding Protein-stimulated Poly(A)-nuclease Activity. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.271.1.432
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