The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
A Leucine Zipper Stabilizes the Pentameric Membrane Domain of Phospholamban and Forms a Coiled-coil Pore Structure
Open accessAbstract
Phospholamban is a phosphoprotein regulator of cardiac sarcoplasmic reticulum which is phosphorylated in response to beta-adrenergic stimulation. Previous results have shown that phospholamban forms Ca2+-selective channels in lipid bilayers. The channel-forming domain has been localized to amino acid residues 26-52, which form a stable pentameric, helical structure. The specific residues responsible for stabilizing the pentameric membrane domain of phospholamban have been identified by mutational analysis. Residues 26-52 were individually mutated to Ala or Phe, and the ability of the resulting mutant to form a pentamer or other oligomer was assessed by SDS-polyacrylamide gel electrophoresis analysis. Replacement of Leu37, Ile40, Leu44, Ile47, or Leu51 by Ala prevented pentamer formation, indicating their essential involvement in the oligomeric assembly. The heptad repeats, and 3-4-residue spacing of the essential amino acids suggest that residues 37-52 adopt a pentameric coiled-coil structure stabilized by a leucine zipper motif formed by the close packing of Leu37, Ile40, Leu44, Ile47, and Leu51. The resulting symmetric structure contains a central pore defined by the hydrophobic surface of the five stabilizing leucine zippers, which are oriented to the interior and form the backbone of the pentamer.
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Cites
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Cited by
Phospholamban: a crucial regulator of cardiac contractility
MacLennan, D. H., & Kranias, E. G. (2003) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1151 · Cited by 1,088
Haghighi, K., Kolokathis, F., Pater, L., Lynch, R. A., Asahi, M., Gramolini, A. O., Fan, G.-C., Tsiapras, D., Hahn, H. S., Adamopoulos, S., et al. (2003) · Journal of Clinical Investigation · doi:10.1172/jci17892 · Cited by 478
Phospholamban: A Prominent Regulator of Myocardial Contractility
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Bishop, N., & Woodman, P. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m009863200 · Cited by 187
Metadata
- Authors
- Simmerman, H. K. B., Kobayashi, Y. M., Autry, J. M., & Jones, L. R.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1996-03
- Volume
- 271
- Issue
- 10
- Pages
- 5941-5946
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2097761188
- Cited by
- 256
- References
- 34
- FWCI
- 5.57
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: A Leucine Zipper Stabilizes the Pentameric Membrane Domain of Phospholamban and Forms a Coiled-coil Pore Structure. https://app.cochat.ai/papers/10.1074/jbc.271.10.5941
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- Simmerman, H. K. B., Kobayashi, Y. M., Autry, J. M., & Jones, L. R. (1996). A Leucine Zipper Stabilizes the Pentameric Membrane Domain of Phospholamban and Forms a Coiled-coil Pore Structure. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.271.10.5941
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