The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.
Expression and Characterization of Inactivating and Activating Mutations in the Human Ca2+-sensing Receptor
Open accessAbstract
Nearly 30 mutations have been identified to date in the coding region of the extracellular calcium-sensing receptor (CaR) that are associated with inherited human hypo- and hypercalcemic disorders. To understand the mechanisms by which the mutations alter the function of the receptor may help to discern the structure-function relationships in terms of ligand-binding and G protein coupling. In the present studies, we transiently expressed eight known CaR mutations in HEK293 cells. The effects of the mutations on extracellular calcium- and gadolinium-elicited increases in the cytosolic calcium concentration were then examined. Seven inactivating mutations, which cause familial hypocalciuric hypercalcemia and neonatal severe hyperparathyroidism, show a reduced functional activity of the receptor because they may 1) reduce its affinity for agonists; 2) prevent conversion of the receptor from a putatively immature, high mannose form into the fully glycosylated and biologically active form of the CaR, in addition to lowering its affinity for agonists; or 3) fail to couple the receptor to and/or activate its respective G protein(s). Conversely, one activating mutation, which causes a form of autosomal dominant hypocalcemia, appears to increase the affinity of the receptor for its agonists.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Multiple Range and Multiple F Tests
Duncan, D. B. (1955) · Biometrics · doi:10.2307/3001478 · Cited by 23,217
SNEDECOR, G. W. (1939) · Soil Science · doi:10.1097/00010694-193902000-00006 · Cited by 19,359
Rapid and efficient site-specific mutagenesis without phenotypic selection.
Kunkel, T. A. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.2.488 · Cited by 7,206
Snedecor, G. W., & Cochran, W. G. (1967) · Cited by 6,573
Cloning and characterization of an extracellular Ca2+-sensing receptor from bovine parathyroid
Brown, E. M., Gamba, G., Riccardi, D., Lombardi, M., Butters, R., Kifor, O., Sun, A., Hediger, M. A., Lytton, J., & Hebert, S. C. (1993) · Nature · doi:10.1038/366575a0 · Cited by 2,643
Self-citationMolecular diversity of glutamate receptors and implications for brain function
Nakanishi, S. (1992) · Science · doi:10.1126/science.1329206 · Cited by 2,543
Pollak, M. R., Brown, E. M., Chou, Y.-H. W., Hebert, S. C., Marx, S. J., Stelnmann, B., Levi, T., Seidman, C. E., & Seidman, J. G. (1993) · Cell · doi:10.1016/0092-8674(93)90617-y · Cited by 1,043
Self-citationA novel rapid assay for chloramphenicol acetyltransferase gene expression
Neumann, J., Morency, C. A., & Russian, K. O. (1987) · BioTechniques · Cited by 749
Brown, E. M. (1991) · Physiological Reviews · doi:10.1152/physrev.1991.71.2.371 · Cited by 690
Self-citation
Cited by
Lytic Cycle of Toxoplasma gondii
Black, M. W., & Boothroyd, J. C. (2000) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.64.3.607-623.2000 · Cited by 554
Advancing human genetics research and drug discovery through exome sequencing of the UK Biobank
Szustakowski, J. D., Balasubramanian, S., Kvikstad, E., Khalid, S., Bronson, P. G., Sasson, A., Wong, E., Liu, D., Wade Davis, J., Haefliger, C., et al. (2021) · Nature Genetics · doi:10.1038/s41588-021-00885-0 · Cited by 401
Hendy, G. N., D'Souza-Li, L., Yang, B., Canaff, L., & Cole, D. E. C. (2000) · Human Mutation · doi:10.1002/1098-1004(200010)16:4<281::aid-humu1>3.0.co;2-a · Cited by 277
Hakimi, S., Dutta, P., & Layton, A. T. (2023) · American Journal of Physiology-Renal Physiology · doi:10.1152/ajprenal.00145.2023 · Cited by 14
Ittisoponpisan, S., & David, A. (2018) · Journal of the Endocrine Society · doi:10.1210/js.2018-00077 · Cited by 8
Metadata
- Authors
- Bai, M., Quinn, S., Trivedi, S., Kifor, O., Pearce, S. H. S., Pollak, M. R., Krapcho, K., Hebert, S. C., & Brown, E. M.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1996-08
- Volume
- 271
- Issue
- 32
- Pages
- 19537-19545
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1972065681
- Cited by
- 422
- References
- 16
- FWCI
- 24.82
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Expression and Characterization of Inactivating and Activating Mutations in the Human Ca2+-sensing Receptor. https://app.cochat.ai/papers/10.1074/jbc.271.32.19537
- The paper
- Bai, M., Quinn, S., Trivedi, S., Kifor, O., Pearce, S. H. S., Pollak, M. R., Krapcho, K., Hebert, S. C., & Brown, E. M. (1996). Expression and Characterization of Inactivating and Activating Mutations in the Human Ca2+-sensing Receptor. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.271.32.19537
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
