The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
Interaction of the Nck Adapter Protein with p21-activated Kinase (PAK1)
Open accessAbstract
The p21-activated kinases (PAKs) link G protein-coupled receptors and growth factor receptors (S. Dharmawardhane, R. H. Daniels, and G. M. Bokoch, submitted for publication) to activation of MAP kinase cascades and to cytoskeletal reorganization (M. A. Sells, U. G. Knaus, D. Ambrose, S. Bagrodia, G. M. Bokoch, and J. Chernoff, submitted for publication). The proteins that interact with PAK to mediate its cellular effects and to couple it to upstream receptors are unknown. We describe here a specific interaction of the Nck adapter molecule with PAK1 both in vitro and in vivo. PAK1 and Nck associate in COS-7 and Swiss 3T3 cells constitutively, but this interaction is strengthened upon platelet-derived growth factor receptor stimulation. We show that Nck binds to PAK1 through its second Src homology 3 (SH3) domain, while PAK1 interacts with Nck via the first proline-rich SH3 binding motif at its amino terminus. The interaction of active PAK1 with Nck leads to the phosphorylation of Nck at multiple sites. Association of Nck with PAK1 may serve to link this important regulatory kinase to cell activation by growth factor receptors.
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A brain serine/threonine protein kinase activated by Cdc42 and Rac1
Manser, E., Leung, T., Salihuddin, H., Zhao, Z.-S., & Lim, L. (1994) · Nature · doi:10.1038/367040a0 · Cited by 1,538
Site-directed mutagenesis of virtually any plasmid by eliminating a unique site
Deng, W. P., & Nickoloff, J. A. (1992) · Analytical Biochemistry · doi:10.1016/0003-2697(92)90280-k · Cited by 1,060
Structural basis for the binding of proline-rich peptides to SH3 domains
Yu, H., Chen, J. K., Feng, S., Dalgarno, D. C., Brauer, A. W., & Schrelber, S. L. (1994) · Cell · doi:10.1016/0092-8674(94)90367-0 · Cited by 1,001
Pawson, T., & Schlessingert, J. (1993) · Current Biology · doi:10.1016/0960-9822(93)90350-w · Cited by 688
Bagrodia, S., Dérijard, B., Davis, R. J., & Cerione, R. A. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.47.27995 · Cited by 635
Tanaka, M., & Herr, W. (1990) · Cell · doi:10.1016/0092-8674(90)90589-7 · Cited by 635
Zhang, S., Han, J., Sells, M. A., Chernoff, J., Knaus, U. G., Ulevitch, R. J., & Bokoch, G. M. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.41.23934 · Cited by 614
Self-citationRegulation of Human Leukocyte p21-Activated Kinases Through G Protein—Coupled Receptors
Knaus, U. G., Morris, S., Dong, H.-J., Chernoff, J., & Bokoch, G. M. (1995) · Science · doi:10.1126/science.7618083 · Cited by 402
Self-citationIdentification of a Mouse p21Cdc42/Rac Activated Kinase
Bagrodia, S., Taylor, S. J., Creasy, C. L., Chernoff, J., & Cerione, R. A. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.39.22731 · Cited by 394
Martin, G. A., Bollag, G., McCormick, F., & Abo, A. (1995) · The EMBO Journal · doi:10.1002/j.1460-2075.1995.tb07189.x · Cited by 364
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BISHOP, A. L., & HALL, A. (2000) · Biochemical Journal · doi:10.1042/bj3480241 · Cited by 2,575
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Virkamäki, A., Ueki, K., & Kahn, C. R. (1999) · Journal of Clinical Investigation · doi:10.1172/jci6609 · Cited by 854
SH3 domains: complexity in moderation
Mayer, B. J. (2001) · Journal of Cell Science · doi:10.1242/jcs.114.7.1253 · Cited by 788
Huber, A. B., Kolodkin, A. L., Ginty, D. D., & Cloutier, J.-F. (2003) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.26.010302.081139 · Cited by 719
Metadata
- Authors
- Bokoch, G. M., Wang, Y., Bohl, B. P., Sells, M. A., Quilliam, L. A., & Knaus, U. G.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1996-10
- Volume
- 271
- Issue
- 42
- Pages
- 25746-25749
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1989987282
- Cited by
- 310
- References
- 21
- FWCI
- 8.91
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Bokoch, G. M., Wang, Y., Bohl, B. P., Sells, M. A., Quilliam, L. A., & Knaus, U. G. (1996). Interaction of the Nck Adapter Protein with p21-activated Kinase (PAK1). Journal of Biological Chemistry. https://doi.org/10.1074/jbc.271.42.25746
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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