The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
On the Mechanism of Fatty Acid-induced Proton Transport by Mitochondrial Uncoupling Protein
Open accessAbstract
Uncoupling protein mediates electrophoretic transport of protons and anions across the inner membrane of brown adipose tissue mitochondria. The mechanism and site of proton transport, the mechanism by which fatty acids activate proton transport, and the relationship between fatty acids and anion transport are unknown. We used fluorescent probes to measure H+ and anion transport in vesicles reconstituted with purified uncoupling protein and carried out a comparative study of the effects of laurate and its close analogue, undecanesulfonate. Undecanesulfonate was transported by uncoupling protein with a Km value similar to that observed for laurate as it activated H+ transport. Both laurate and undecanesulfonate inhibited Cl- with competitive kinetics. Undecanesulfonate inhibited laurate-induced H+ transport with competitive kinetics. Undecanesulfonate and laurate differed in two important respects. (i) Laurate caused uncoupling protein-mediated H+ transport, whereas undecanesulfonate did not. (ii) Lauric acid was rapidly transported across the bilayer by nonionic diffusion, whereas undecanesulfonic was not. We infer that the role of uncoupling protein in H+ transport is to transport fatty acid anions and that fatty acids induce H+ transport because they can diffuse electroneutrally across the membrane. According to this hypothesis, uncoupling protein is a pure anion porter and does not transport protons; rather it is designed to enable fatty acids to behave as cycling protonophores.
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Cites
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Kamp, F., & Hamilton, J. A. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.23.11367 · Cited by 299
Mechanism and evolution of the uncoupling protein of brown adipose tissue
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Cited by
Myocardial Substrate Metabolism in the Normal and Failing Heart
Stanley, W. C., Recchia, F. A., & Lopaschuk, G. D. (2005) · Physiological Reviews · doi:10.1152/physrev.00006.2004 · Cited by 2,048
Uncoupling: new approaches to an old problem of bioenergetics
Skulachev, V. P. (1998) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/s0005-2728(97)00091-1 · Cited by 893
Mitochondrial proton and electron leaks
Jastroch, M., Divakaruni, A. S., Mookerjee, S., Treberg, J. R., & Brand, M. D. (2010) · Essays in Biochemistry · doi:10.1042/bse0470053 · Cited by 829
Energy metabolism in uncoupling protein 3 gene knockout mice
Vidal-Puig, A. J., Grujic, D., Zhang, C.-Y., Hagen, T., Boss, O., Ido, Y., Szczepanik, A., Wade, J., Mootha, V., Cortright, R., et al. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m910179199 · Cited by 677
The mitochondrial uncoupling-protein homologues
Krauss, S., Zhang, C.-Y., & Lowell, B. B. (2005) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1592 · Cited by 663
Metadata
- Authors
- Garlid, K. D., Orosz, D. E., Modrianský, M., Vassanelli, S., & Jezek, P.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1996-02
- Volume
- 271
- Issue
- 5
- Pages
- 2615-2620
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2007819480
- Cited by
- 327
- References
- 24
- FWCI
- 8.66
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Garlid, K. D., Orosz, D. E., Modrianský, M., Vassanelli, S., & Jezek, P. (1996). On the Mechanism of Fatty Acid-induced Proton Transport by Mitochondrial Uncoupling Protein. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.271.5.2615
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
