The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
The Activity of the Epithelial Sodium Channel Is Regulated by Clathrin-mediated Endocytosis
Open accessAbstract
Activity of the epithelial sodium channel (ENaC) is a key determinant of sodium homeostasis and blood pressure. Liddle's syndrome, an inherited form of hypertension, is caused by mutations that delete or alter PY domains in the carboxyl termini of beta or gamma ENaC subunits, leading to increased channel activity. In this study we investigated the mechanism of this effect by analysis of wild-type and mutant ENaC activity in Xenopus oocytes. By inhibiting insertion of new channels into the plasma membrane with brefeldin A, we demonstrate that the half-life of the activity of channels containing Liddle's mutations is markedly prolonged compared with wild-type channels (t1/2 of 30 h in mutant versus 3.6 in wild-type, p < 0.001). We investigated the involvement of clathrin-coated pit-mediated endocytosis by co-expressing a dominant-negative dynamin mutant with wild-type ENaC in oocytes. Expression of this specific inhibitor of endocytosis leads to a large increase in the activity of wild-type channels, demonstrating that normal turnover of this channel is through the clathrin-coated pit pathway. In contrast, co-expression of Liddle's mutations and dynamin mutants leads to no further increase in channel activity, consistent with one of the effects of Liddle's mutations being the loss of endocytosis of these channels. These findings demonstrate the normal mechanism of turnover of ENaC from the cell surface and demonstrate a mechanism that can account for the increased number of channels in the plasma membrane seen in Liddle's syndrome.
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Cites
Amiloride-sensitive epithelial Na+ channel is made of three homologous subunits
Canessa, C. M., Schild, L., Buell, G., Thorens, B., Gautschi, I., Horisberger, J.-D., & Rossier, B. C. (1994) · Nature · doi:10.1038/367463a0 · Cited by 1,961
Self-citationShimkets, R. A., Warnock, D. G., Bositis, C. M., Nelson-Williams, C., Hansson, J. H., Schambelan, M., Gill, J. R., Ulick, S., Milora, R. V., Findling, J. W., et al. (1994) · Cell · doi:10.1016/0092-8674(94)90250-x · Cited by 1,259
Self-citationInduction of mutant dynamin specifically blocks endocytic coated vesicle formation.
Damke, H., Baba, T., Warnock, D. E., & Schmid, S. L. (1994) · The Journal of cell biology · doi:10.1083/jcb.127.4.915 · Cited by 1,204
Staub, O., Dho, S., Henry, P., Correa, J., Ishikawa, T., McGlade, J., & Rotin, D. (1996) · The EMBO Journal · doi:10.1002/j.1460-2075.1996.tb00593.x · Cited by 851
Signal-Dependent Membrane Protein Trafficking in the Endocytic Pathway
Trowbridge, I. S., Collawn, J. F., & Hopkins, C. R. (1993) · Annual Review of Cell Biology · doi:10.1146/annurev.cb.09.110193.001021 · Cited by 804
Tubular membrane invaginations coated by dynamin rings are induced by GTP-γS in nerve terminals
Takei, K., McPherson, P. S., Schmid, S. L., & Camilli, P. D. (1995) · Nature · doi:10.1038/374186a0 · Cited by 767
Hansson, J. H., Nelson-Williams, C., Suzuki, H., Schild, L., Shimkets, R., Lu, Y., Canessa, C., Iwasaki, T., Rossier, B., & Lifton, R. P. (1995) · Nature Genetics · doi:10.1038/ng0995-76 · Cited by 754
Self-citationFirsov, D., Schild, L., Gautschi, I., Mérillat, A.-M., Schneeberger, E., & Rossier, B. C. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.26.15370 · Cited by 421
Schild, L., Lu, Y., Gautschi, I., Schneeberger, E., Lifton, R. P., & Rossier, B. C. (1996) · The EMBO Journal · doi:10.1002/j.1460-2075.1996.tb00594.x · Cited by 398
Self-citationBrefeldin A causes a microtubule-mediated fusion of the trans-Golgi network and early endosomes
Wood, S. A., Park, J. E., & Brown, W. J. (1991) · Cell · doi:10.1016/0092-8674(91)90533-5 · Cited by 384
Cited by
Molecular Mechanisms of Human Hypertension
Lifton, R. P., Gharavi, A. G., & Geller, D. S. (2001) · Cell · doi:10.1016/s0092-8674(01)00241-0 · Cited by 1,686
Self-citationDynamin and Its Role in Membrane Fission
Hinshaw, J. E. (2000) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.16.1.483 · Cited by 699
Electrolyte transport in the mammalian colon: mechanisms and implications for disease
Kunzelmann, K., & Mall, M. (2002) · Physiological Reviews · doi:10.1152/physrev.00026.2001 · Cited by 655
Gettin’ down with ubiquitin: turning off cell-surface receptors, transporters and channels
Hicke, L. (1999) · Trends in Cell Biology · doi:10.1016/s0962-8924(98)01491-3 · Cited by 462
Structure and Regulation of Amiloride-Sensitive Sodium Channels
de la Rosa, D. A., Canessa, C. M., Fyfe, G. K., & Zhang, P. (2000) · Annual Review of Physiology · doi:10.1146/annurev.physiol.62.1.573 · Cited by 332
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Metadata
- Authors
- Shimkets, R. A., Lifton, R. P., & Canessa, C. M.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1997-10
- Volume
- 272
- Issue
- 41
- Pages
- 25537-25541
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2040483955
- Cited by
- 271
- References
- 21
- FWCI
- 7.65
- Type
- journal article
- Language
- en
- Open access
- hybrid
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