The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
Transcriptional Regulation of The Human Monocyte Chemoattractant Protein-1 Gene
Open accessAbstract
Human monocyte chemoattractant protein-1 (human MCP-1) mRNA accumulated in THP-1 cells 2 h after lipopolysaccharide (LPS) stimulation. DNase I footprinting revealed that LPS stimulation induced protein binding to the two closely located NF-kappaB sites, A1 and A2. By electrophoretic gel mobility shift assay and supershift assay, the binding of (p65)2, c-Rel/p65, p50/p65, and p50/c-Rel to the A2 oligonucleotide probe was detected after LPS stimulation. In contrast, 12-o-tetradecanoylphorbol 13-acetate did not induce a significant amount of MCP-1 mRNA in THP-1 cells 2 h after stimulation, and only p50/p65 bound to the A2 probe. trans-Activity of each NF-kappaB/Rel dimer was investigated by transfecting P19 cells with p65, p50, and/or c-Rel expression vectors, and a luciferase construct containing the enhancer region of the human MCP-1 gene. Expression of recombinant p65 or p65 and c-Rel resulted in elevated luciferase activities, indicating that (p65)2 and c-Rel/p65 had trans-activity. The binding of (p65)2 and/or c-Rel/p65 to the A2 probe was also detected from 12-o-tetradecanoylphorbol 13-acetate-stimulated HeLa, HOS, and A172 cells in which expression of MCP-1 mRNA was elevated. Finally, the role of the A1 site was investigated. Both (p65)2 and c-Rel/p65 bound to the A1 probe by electrophoretic mobility shift assay and a mutation in the A1 or A2 site resulted in a loss of the enhancer activity. These results suggest that the binding of (p65)2 and c-Rel/p65 to the A1 and A2 sites of this gene is important for the tissue- and stimulus-specific transcription of the human MCP-1 gene.
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Cites
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Increased expression of nuclear factor-κB in bronchial biopsies from smokers and patients with COPD
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Davis, K. M., Nakamura, S., & Weiser, J. N. (2011) · Journal of Clinical Investigation · doi:10.1172/jci57761 · Cited by 190
Prolonged Activation of NF-κB Following Traumatic Brain Injury in Rats
NONAKA, M., CHEN, X.-H., PIERCE, J. E. S., LEONI, M. J., McINTOSH, T. K., WOLF, J. A., & SMITH, D. H. (1999) · Journal of Neurotrauma · doi:10.1089/neu.1999.16.1023 · Cited by 176
Chen, I.-Y., Chang, S. C., Wu, H.-Y., Yu, T.-C., Wei, W.-C., Lin, S., Chien, C.-L., & Chang, M.-F. (2010) · Journal of Virology · doi:10.1128/jvi.02560-09 · Cited by 141
Metadata
- Authors
- Ueda, A., Ishigatsubo, Y., Okubo, T., & Yoshimura, T.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1997-12
- Volume
- 272
- Issue
- 49
- Pages
- 31092-31099
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2079866862
- Cited by
- 309
- References
- 49
- FWCI
- 6.55
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Ueda, A., Ishigatsubo, Y., Okubo, T., & Yoshimura, T. (1997). Transcriptional Regulation of The Human Monocyte Chemoattractant Protein-1 Gene. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.272.49.31092
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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