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Reconstitution of Human Excision Nuclease with Recombinant XPF-ERCC1 Complex

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Bessho, Tadayoshi, Sancar, Aziz, Thompson, Larry H., Thelen, Michael P.·1997·Journal of Biological Chemistry

Abstract

The human XPF-ERCC1 protein complex is one of several factors known to be required for general nucleotide excision repair. Genetic data indicate that both proteins of this complex are necessary for the repair of interstrand cross-links, perhaps via recombination. To determine whether XPF-ERCC1 completes a set of six proteins that are sufficient to carry out excision repair, the human XPF and ERCC1 cDNAs were coexpressed in Sf21 insect cells from a baculovirus vector. The purified complex contained the anticipated 5' junction-specific endonuclease activity that is stimulated through a direct interaction between XPF and replication protein A (RPA). The recombinant complex also complemented extracts of XP-F cells and Chinese hamster ovary mutants assigned to complementation groups 1, 4, and 11. Furthermore, reconstitution of the human excision nuclease was observed with a mixture of five repair factors (XPA, XPC, XPG, TFIIH, and RPA) and the recombinant XPF-ERCC1, thus verifying that no additional protein factors are needed for the specific dual incisions characteristic of human excision repair.

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Metadata

Authors
Bessho, T., Sancar, A., Thompson, L. H., & Thelen, M. P.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1997-02
Volume
272
Issue
6
Pages
3833-3837
ISSN
0021-9258 (ISSN-L)
OpenAlex
W2000983378
Cited by
169
References
4
FWCI
5.51
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Reconstitution of Human Excision Nuclease with Recombinant XPF-ERCC1 Complex. https://app.cochat.ai/papers/10.1074/jbc.272.6.3833
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Bessho, T., Sancar, A., Thompson, L. H., & Thelen, M. P. (1997). Reconstitution of Human Excision Nuclease with Recombinant XPF-ERCC1 Complex. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.272.6.3833

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

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