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Reaction Mechanism of Fluoroacetate Dehalogenase from Moraxella sp. B
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“The latter was subsequently hydrolyzed by a water molecule activated by His-272 (71).”
From Toward the development of a molecular toolkit for the microbial remediation of per-and polyfluoroalkyl substances (2024) · cites this paper as [71]
In the citing paper
“The catalytic mechanism for hydrolysis of fluoroacetate by haloacid dehalogenases is an SN2 displacement reaction involving two steps, namely, the formation of an enzyme-ester intermediate followed by the subsequent hydrolysis of the ester intermediate via an activated water molecule (71, 77) (Fig. 2).”
From Toward the development of a molecular toolkit for the microbial remediation of per-and polyfluoroalkyl substances (2024) · cites this paper as [71]
In the citing paper
“Site-directed mutagenesis and molecular simulations demonstrated the essential role of aspartate (Asp-105; H-1 numbering) and histidine (His-272; H-1 numbering) in the catalytic process.”
From Toward the development of a molecular toolkit for the microbial remediation of per-and polyfluoroalkyl substances (2024) · cites this paper as [71]
In the citing paper
“Asp-105 serves as a nucleophile to attack the α-carbon of fluoroacetate, leading to the release of a fluoride anion and the formation of an enzyme-ester intermediate.”
From Toward the development of a molecular toolkit for the microbial remediation of per-and polyfluoroalkyl substances (2024) · cites this paper as [71]
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Metadata
- Authors
- Liu, J.-Q., Kurihara, T., Ichiyama, S., Miyagi, M., Tsunasawa, S., Kawasaki, H., Soda, K., & Esaki, N.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1998-11
- Volume
- 273
- Issue
- 47
- Pages
- 30897-30902
- ISSN
- 0021-9258
- Cited by
- 88
- References
- 0
- Language
- en
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- CoChat. (2026). Citation check: Reaction Mechanism of Fluoroacetate Dehalogenase from Moraxella sp. B. https://app.cochat.ai/papers/10.1074/jbc.273.47.30897
- The paper
- Liu, J.-Q., Kurihara, T., Ichiyama, S., Miyagi, M., Tsunasawa, S., Kawasaki, H., Soda, K., & Esaki, N. (1998). Reaction Mechanism of Fluoroacetate Dehalogenase from Moraxella sp. B. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.273.47.30897