The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
The Specificity of the CRM1-Rev Nuclear Export Signal Interaction Is Mediated by RanGTP
Open accessAbstract
Nuclear export of intron-containing human immunodeficiency virus type 1 (HIV-1) RNA is mediated by the viral Rev protein that contains both an RNA binding domain specific for the viral Rev response element (RRE) and a nuclear export signal (NES). The cellular CRM1 (Exportin1) protein functions as a nuclear export receptor for proteins carrying a Rev-like NES in a process that also requires the GTP bound form of the Ran GTPase. Using purified recombinant factors, we show by co-precipitation, gel mobility shift and protein footprinting assays that full-length Rev protein interacts directly with CRM1 in vitro independently of both the integrity of the characteristic leucine residues of the NES and the presence of the cytotoxin leptomycin B (LMB). Addition of RanGTP induces the formation of an RRE-Rev-CRM1-RanGTP complex that is sensitive to LMB, NES mutations, and Ran being charged with GTP. Within this complex, CRM1 is readily cross-linked to Cys89 near the NES of Rev. By protein footprinting, we demonstrate that the NES of Rev and two regions in CRM1 become inaccessible to endoproteinases upon binding suggesting that these regions are involved in protein-protein interactions. Our data are consistent with a model in which CRM1 is the nuclear export receptor for the Rev-RRE ribonucleoprotein complex and that RanGTP binds to a preformed Rev-CRM1 complex and specifies a functional interaction with the NES.
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“To achieve this, the Rev/RRE complex recruits a host protein, the cellular chromosomal maintenance 1 (CRM1, also known as Exportin 1; XpoI) and Ran (RAs-related nuclear protein) also known as GTP-binding nuclear protein, to form the nuclear export complex that successfully exports these RNAs from nucleus to the cytoplasm (Fig. 9 and Table 1) (Neville et al. 1997, Askjaer et al. 1998, Bai et al. 2”
From Beyond reverse transcription: molecular mechanisms and emerging paradigms in retroviral replication (2026) · cites this paper as Askjaer et al. 1998
Citations
Cites
CRM1 Is an Export Receptor for Leucine-Rich Nuclear Export Signals
Fornerod, M. W. J., Ohno, M., Yoshida, M., & Mattaj, I. W. (1997) · Cell · doi:10.1016/s0092-8674(00)80371-2 · Cited by 2,086
CRM1 is responsible for intracellular transport mediated by the nuclear export signal
Fukuda, M., Asano, S., Nakamura, T., Adachi, M., Yoshida, M., Yanagida, M., & Nishida, E. (1997) · Nature · doi:10.1038/36894 · Cited by 1,213
Identification of a signal for rapid export of proteins from the nucleus
Wen, W., Meinkotht, J. L., Tsien, R. Y., & Taylor, S. S. (1995) · Cell · doi:10.1016/0092-8674(95)90435-2 · Cited by 1,190
Nucleocytoplasmic transport: the soluble phase
Mattaj, I. W., & Englmeier, L. (1998) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.67.1.265 · Cited by 1,175
Self-citationExportin 1 (Crm1p) Is an Essential Nuclear Export Factor
Stade, K., Ford, C. S., Guthrie, C., & Weis, K. (1997) · Cell · doi:10.1016/s0092-8674(00)80370-0 · Cited by 1,145
Fischer, U., Huber, J., Boelens, W. C., Mattaj, I. W., & Lührmann, R. (1995) · Cell · doi:10.1016/0092-8674(95)90436-0 · Cited by 1,140
Malim, M. H., Böhnlein, S., Hauber, J., & Cullen, B. R. (1989) · Cell · doi:10.1016/0092-8674(89)90416-9 · Cited by 732
Evidence for a Role of CRM1 in Signal-Mediated Nuclear Protein Export
Ossareh‐Nazari, B., Bachelerie, F., & Dargemont, C. (1997) · Science · doi:10.1126/science.278.5335.141 · Cited by 713
Catalysis of guanine nucleotide exchange on Ran by the mitotic regulator RCC1
Bischoff, F. R., & Ponstingl, H. (1991) · Nature · doi:10.1038/354080a0 · Cited by 711
Isolation of a protein that is essential for the first step of nuclear protein import
Görlich, D. (1994) · Cell · doi:10.1016/0092-8674(94)90067-1 · Cited by 695
Flagged first, then by citations
Research works printed but not linked: 5
- Kalland, 1994, Mol. Cell. Biol., 14, 7436
- Perkins, 1989, J. Acquired Immune Defic. Syndr., 2, 256
- Fankhauser, 1991, Mol. Cell. Biol., 11, 2567
- Olsen, 1991, J. Acquired Immune Defic. Syndr., 4, 558
- Izaurralde, 1998, RNA, 4, 351
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HIV-1 Infection Alters Post-Transcriptional Processing of HTLV-1 RNA in MT-2 Cells
Liu, H., Wei, S., 한진관, Li, H., Liu, Y., Jia, L., Wang, X., Zhang, B., & Li, L. (2026) · Microorganisms · doi:10.3390/microorganisms14102359
Huntingtin Nuclear Localization: Current Insights into Mechanism and Regulation
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HIV-1 and the Nucleolus: A Role for Nucleophosmin/NPM1 in Viral Replication: A Dissertation
Schmidt, T. E. (2013) · The Journal of the American Medical Association (JAMA) Network (American Medical Association) · doi:10.13028/m2mk6v
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Metadata
- Authors
- Askjaer, P., Jensen, T. H., Nilsson, J., Englmeier, L., & Kjems, J.
- Affiliations
- Aarhus University (DK)
- Corresponding author
- Kjems, Jørgen
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1998-12
- Volume
- 273
- Issue
- 50
- Pages
- 33414-33422
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1979451022
- Cited by
- 219
- References
- 49
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 6 · 2023: 2 · 2022: 7 · 2021: 3 · 2020: 7 · 2019: 4 · 2018: 3 · 2017: 3 · 2016: 6 · 2015: 5 · 2014: 10 · 2013: 8 · 2012: 16
- FWCI
- 4.83
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Kræftens Bekæmpelse
- Karen Elise Jensens Fond
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Keywords
- CRM1, Rev protein, nuclear export signal, Ran GTPase, Rev response element, protein-protein interaction, leptomycin B, nuclear export receptor
- MeSH terms
- Exportin 1 Protein, Base Sequence, Carrier Proteins, Cell Nucleus, Guanosine Triphosphate, Recombinant Proteins, Ultraviolet Rays, Signal Transduction, Gene Products, rev, DNA Primers, Receptors, Cytoplasmic and Nuclear, Karyopherins
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