The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Co-translocation of a Periplasmic Enzyme Complex by a Hitchhiker Mechanism through the Bacterial Tat Pathway
Open accessAbstract
Bacterial periplasmic nickel-containing hydrogenases are composed of a small subunit containing a twin-arginine signal sequence and a large subunit devoid of an export signal. To understand how the large subunit is translocated into the periplasm, we cloned the hyb operon encoding the hydrogenase 2 of Escherichia coli, constructed a deletion mutant, and studied the mechanism of translocation of hydrogenase 2. The small subunit (HybO) or the large subunit (HybC) accumulated in the cytoplasm as a precursor when either of them was expressed in the absence of the other subunit. Therefore, contrary to most classical secretory proteins, the signal sequence of the small subunit itself is not sufficient for membrane targeting and translocation if the large subunit is missing. On the other hand, the small subunit was required not only for membrane targeting of the large subunit, but also for the acquisition of nickel by the large subunit. Most interestingly, the signal sequence of the small subunit determines whether the large subunit follows the Sec or the twin-arginine translocation pathway. Taken together, these results provide for the first time compelling evidence for a naturally occurring hitchhiker co-translocation mechanism in bacteria.
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Cited by
Oxidation of Reduced Inorganic Sulfur Compounds by Bacteria: Emergence of a Common Mechanism?
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Protein translocation across biological membranes
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Enzymology and bioenergetics of respiratory nitrite ammonification
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The Bacterial Twin-Arginine Translocation Pathway
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Self-citation
Metadata
- Authors
- Rodrigue, A., Chanal, A., Beck, K., Müller, M., & Wu, L.-F.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1999-05
- Volume
- 274
- Issue
- 19
- Pages
- 13223-13228
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1966082253
- Cited by
- 267
- References
- 30
- FWCI
- 7.77
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Rodrigue, A., Chanal, A., Beck, K., Müller, M., & Wu, L.-F. (1999). Co-translocation of a Periplasmic Enzyme Complex by a Hitchhiker Mechanism through the Bacterial Tat Pathway. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.274.19.13223
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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