The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
Influenza Viruses Select Ordered Lipid Domains during Budding from the Plasma Membrane
Open accessAbstract
During the budding of enveloped viruses from the plasma membrane, the lipids are not randomly incorporated into the envelope, but virions seem to have a lipid composition different from the host membrane. Here, we have analyzed lipid assemblies in three different viruses: fowl plague virus (FPV) from the influenza virus family, vesicular stomatitis virus (VSV), and Semliki Forest virus (SFV). Analysis of detergent extractability of proteins, cholesterol, phosphoglycerolipids, and sphingomyelin in virions showed that FPV contains high amounts of detergent-insoluble complexes, whereas such complexes are largely absent from VSV or SFV. Cholesterol depletion from the viral envelope by methyl-beta-cyclodextrin results in increased solubility of sphingomyelin and of the glycoproteins in the FPV envelope. This biochemical behavior suggests that so-called raft-lipid domains are selectively incorporated into the influenza virus envelope. The "fluidity" of the FPV envelope, as measured by the fluorescence polarization of diphenylhexatriene, was significantly lower than compared with VSV or SFV. Furthermore, influenza virus hemagglutinin incorporated into the envelope of recombinant VSV was largely detergent-soluble, indicating the depletion of raft-lipid assemblies from this membrane. The results provide a model for lipid selectivity during virus budding and support the view of lipid rafts as cholesterol-dependent, ordered domains in biological membranes.
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Van Damme, N., Goff, D., Katsura, C., Jorgenson, R. L., Mitchell, R., Johnson, M. C., Stephens, E. B., & Guatelli, J. (2008) · Cell Host & Microbe · doi:10.1016/j.chom.2008.03.001 · Cited by 1,050
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Virus Entry, Assembly, Budding, and Membrane Rafts
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The role of cholesterol in membrane fusion
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Metadata
- Authors
- Scheiffele, P., Rietveld, A., Wilk, T., & Simons, K.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1999-01
- Volume
- 274
- Issue
- 4
- Pages
- 2038-2044
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2140900740
- Cited by
- 448
- References
- 43
- FWCI
- 9.57
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Scheiffele, P., Rietveld, A., Wilk, T., & Simons, K. (1999). Influenza Viruses Select Ordered Lipid Domains during Budding from the Plasma Membrane. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.274.4.2038
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
