The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Reconstitution of Proliferating Cell Nuclear Antigen-dependent Repair of Apurinic/Apyrimidinic Sites with Purified Human Proteins
Open accessAbstract
An apurinic/apyrimidinic (AP) site is one of the most abundant lesions spontaneously generated in living cells and is also a reaction intermediate in base excision repair. In higher eukaryotes, there are two alternative pathways for base excision repair: a DNA polymerase beta-dependent pathway and a proliferating cell nuclear antigen (PCNA)-dependent pathway. Here we have reconstituted PCNA-dependent repair of AP sites with six purified human proteins: AP endonuclease, replication factor C, PCNA, flap endonuclease 1 (FEN1), DNA polymerase delta, and DNA ligase I. The length of nucleotides replaced during the repair reaction (patch size) was predominantly two nucleotides, although longer patches of up to seven nucleotides could be detected. Neither replication protein A nor Ku70/80 enhanced the repair activity in this system. Disruption of the PCNA-binding site of either FEN1 or DNA ligase I significantly reduced efficiency of AP site repair but did not affect repair patch size.
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Cites
Mammalian DNA nucleotide excision repair reconstituted with purified protein components
Aboussekhra, A., Biggerstaff, M., Shivji, M. K. K., Vilpo, J. A., Moncollin, V., Podust, V. N., Protić, M., Hübscher, U., Egly, J.-M., & Wood, R. D. (1995) · Cell · doi:10.1016/0092-8674(95)90289-9 · Cited by 837
Excision of Deoxyribose Phosphate Residues by DNA Polymerase β During DNA Repair
Matsumoto, Y., & Kim, K. (1995) · Science · doi:10.1126/science.7624801 · Cited by 683
Self-citationProliferating Cell Nuclear Antigen Facilitates Excision in Long-patch Base Excision Repair
Gary, R., Kim, K., Cornelius, H. L., Park, M. S., & Matsumoto, Y. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.7.4354 · Cited by 170
Self-citation
Cited by
Repair of Endogenous DNA Damage
LINDAHL, T., & BARNES, D. E. (2000) · Cold Spring Harbor Symposia on Quantitative Biology · doi:10.1101/sqb.2000.65.127 · Cited by 714
Coordination of dual incision and repair synthesis in human nucleotide excision repair
Staresincic, L., Fagbemi, A. F., Enzlin, J. H., Gourdin, A. M., Wijgers, N., Dunand‐Sauthier, I., Giglia‐Mari, G., Clarkson, S. G., Vermeulen, W., & Schärer, O. D. (2009) · The EMBO Journal · doi:10.1038/emboj.2009.49 · Cited by 287
Base excision repair in nuclear and mitochondrial DNA
Dianov, G. L., Souza-Pinto, N., Nyaga, S. G., Thybo, T., Stevnsner, T., & Bohr, V. A. (2001) · Progress in nucleic acid research and molecular biology · doi:10.1016/s0079-6603(01)68107-8 · Cited by 174
Long‐patch DNA repair synthesis during base excision repair in mammalian cells
Sattler, U., Frit, P., Salles, B., & Calsou, P. (2003) · The EMBO Reports · doi:10.1038/sj.embor.embor796 · Cited by 89
Friedrich-Heineken, E., Toueille, M., Tännler, B., Bürki, C., Ferrari, E., Hottiger, M. O., & Hübscher, U. (2005) · Journal of Molecular Biology · doi:10.1016/j.jmb.2005.09.018 · Cited by 76
Metadata
- Authors
- Matsumoto, Y., Kim, K., Hurwitz, J., Gary, R., Levin, D. S., Tomkinson, A. E., & Park, M. S.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1999-11
- Volume
- 274
- Issue
- 47
- Pages
- 33703-33708
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1978124987
- Cited by
- 175
- References
- 3
- FWCI
- 6.52
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Matsumoto, Y., Kim, K., Hurwitz, J., Gary, R., Levin, D. S., Tomkinson, A. E., & Park, M. S. (1999). Reconstitution of Proliferating Cell Nuclear Antigen-dependent Repair of Apurinic/Apyrimidinic Sites with Purified Human Proteins. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.274.47.33703
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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