The DOI matches its Crossref record; 58 of the 58 references it cites were found in a registry or an index.
Molecular Cloning and Characterization of a Mitochondrial Selenocysteine-containing Thioredoxin Reductase from Rat Liver
Open accessAbstract
A thioredoxin reductase (TrxR), named here TrxR2, that did not react with antibodies to the previously identified TrxR (now named TrxR1) was purified from rat liver. Like TrxR1, TrxR2 was a dimeric enzyme containing selenocysteine (Secys) as the COOH-terminal penultimate residue. A cDNA encoding TrxR2 was cloned from rat liver; the open reading frame predicts a polypeptide of 526 amino acids with a COOH-terminal Gly-Cys-Secys-Gly motif provided that an in-frame TGA codon encodes Secys. The 3'-untranslated region of the cDNA contains a canonical Secys insertion sequence element. The deduced amino acid sequence of TrxR2 shows 54% identity to that of TrxR1 and contained 36 additional residues upstream of the experimentally determined NH2-terminal sequence. The sequence of this 36-residue region is typical of that of a mitochondrial leader peptide. Immunoblot analysis confirmed that TrxR2 is localized almost exclusively in mitochondria, whereas TrxR1 is a cytosolic protein. Unlike TrxR1, which was expressed at a level of 0.6 to 1.6 microgram/milligram of total soluble protein in all rat tissues examined, TrxR2 was relatively abundant (0.3 to 0.6 microgram/mg) only in liver, kidney, adrenal gland, and heart. The specific localization of TrxR2 in mitochondria, together with the previous identification of mitochondria-specific thioredoxin and thioredoxin-dependent peroxidase, suggest that these three proteins provide a primary line of defense against H2O2 produced by the mitochondrial respiratory chain.
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Self-citationThioredoxin and Related Molecules–From Biology to Health and Disease
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Peroxiredoxin functions as a peroxidase and a regulator and sensor of local peroxides
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Self-citationPGC-1α regulates the mitochondrial antioxidant defense system in vascular endothelial cells
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Metadata
- Authors
- Lee, S.-R., Kim, J.-R., Kwon, K.-S., Yoon, H. W., Levine, R. L., Ginsburg, A., & Rhee, S. G.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1999-02
- Volume
- 274
- Issue
- 8
- Pages
- 4722-4734
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1983397667
- Cited by
- 274
- References
- 58
- FWCI
- 8.81
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Lee, S.-R., Kim, J.-R., Kwon, K.-S., Yoon, H. W., Levine, R. L., Ginsburg, A., & Rhee, S. G. (1999). Molecular Cloning and Characterization of a Mitochondrial Selenocysteine-containing Thioredoxin Reductase from Rat Liver. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.274.8.4722
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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