The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Smurf2 Is a Ubiquitin E3 Ligase Mediating Proteasome-dependent Degradation of Smad2 in Transforming Growth Factor-β Signaling
Open accessAbstract
Smads are important intracellular signaling effectors for transforming growth factor-beta (TGF-beta) and related factors. Proper TGF-beta signaling requires precise control of Smad functions. In this study, we have identified a novel HECT class ubiquitin E3 ligase, designated Smurf2, that negatively regulates Smad2 signaling. In both yeast two-hybrid and in vitro binding assays, we found that Smurf2 could interact with receptor-activated Smads (R-Smads), including Smad1, Smad2, and Smad3 but not Smad4. Ectopic expression of Smurf2 was sufficient to reduce the steady-state levels of Smad1 and Smad2 but not Smad3 or Smad4. Significantly, Smurf2 displayed preference to Smad2 as its target for degradation. Furthermore, Smurf2 exhibited higher binding affinity to activated Smad2 upon TGF-beta stimulation. The ability of Smurf2 to promote Smad2 destruction required the HECT catalytic activity of Smurf2 and depended on the proteasome-dependent pathway. Consistent with these results, Smurf2 potently reduced the transcriptional activity of Smad2. These data suggest that a ubiquitin/proteasome-dependent mechanism is important for proper regulation of TGF-beta signaling.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Controlling TGF-beta signaling.
Massagué, J., & Chen, Y. (2000) · PubMed · Cited by 1,780
Massagué, J., & Chen, Y.-G. (2000) · Genes & Development · doi:10.1101/gad.14.6.627 · Cited by 1,575
Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2
Gyuris, J. (1993) · Cell · doi:10.1016/0092-8674(93)90498-f · Cited by 1,522
Transcriptional Activators of TGF-β Responses: Smads
Derynck, R., Zhang, Y., & Feng, X.-H. (1998) · Cell · doi:10.1016/s0092-8674(00)81696-7 · Cited by 1,029
Self-citationA mechanism of repression of TGFbeta / Smad signaling by oncogenic Ras
Kretzschmar, M., Doody, J., Timokhina, I., & Massague, J. (1999) · Genes & Development · doi:10.1101/gad.13.7.804 · Cited by 1,004
Ubiquitin-mediated proteolysis: biological regulation via destruction
Ciechanover, A., Orian, A., & Schwartz, A. L. (2000) · BioEssays · doi:10.1002/(sici)1521-1878(200005)22:5<442::aid-bies6>3.0.co;2-q · Cited by 853
A SMAD ubiquitin ligase targets the BMP pathway and affects embryonic pattern formation
Zhu, H., Kavsak, P., Abdollah, S., Wrana, J. L., & Thomsen, G. H. (1999) · Nature · doi:10.1038/23293 · Cited by 845
Inhibition of transforming growth factor-β/SMAD signalling by the interferon-γ/STAT pathway
Ulloa, L., Doody, J., & Massagué, J. (1999) · Nature · doi:10.1038/17826 · Cited by 812
Smad3 and Smad4 cooperate with c-Jun/c-Fos to mediate TGF-β-induced transcription
Zhang, Y., Feng, X.-H., & Derynck, R. (1998) · Nature · doi:10.1038/29814 · Cited by 809
Self-citationA human Mad protein acting as a BMP-regulated transcriptional activator
Liu, F., Hata, A., Baker, J. C., Doody, J., Cárcamo, J., Harland, R. M., & Massagué, J. (1996) · Nature · doi:10.1038/381620a0 · Cited by 671
Cited by
Regulation of cell polarity and protrusion formation by targeting RhoA for degradation
Wang, H.-R., Zhang, Y., Ozdamar, B., Ogunjimi, A. A., Alexandrova, E., Thomsen, G. H., & Wrana, J. L. (2003) · Science · doi:10.1126/science.1090772 · Cited by 551
Signal transduction of bone morphogenetic protein receptors
Nohe, A. (2004) · Cellular Signalling · doi:10.1016/j.cellsig.2003.08.011 · Cited by 514
Regulation of TGF-β Family Signaling by Inhibitory Smads
Miyazawa, K., & Miyazono, K. (2017) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a022095 · Cited by 439
Zhang, T., Wang, X.-F., Wang, Z.-C., Lou, D., Fang, Q.-Q., Hu, Y.-Y., Zhao, W.-Y., Zhang, L.-Y., Wu, L.-H., & Tan, W.-Q. (2020) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2020.110287 · Cited by 438
Intracellular BMP Signaling Regulation in Vertebrates: Pathway or Network?
von Bubnoff, A., & Cho, K. W. Y. (2001) · Developmental Biology · doi:10.1006/dbio.2001.0388 · Cited by 393
Metadata
- Authors
- Lin, X., Liang, M., & Feng, X.-H.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2000-11
- Volume
- 275
- Issue
- 47
- Pages
- 36818-36822
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1988810849
- Cited by
- 489
- References
- 36
- FWCI
- 8.34
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Smurf2 Is a Ubiquitin E3 Ligase Mediating Proteasome-dependent Degradation of Smad2 in Transforming Growth Factor-β Signaling. https://app.cochat.ai/papers/10.1074/jbc.c000580200
- The paper
- Lin, X., Liang, M., & Feng, X.-H. (2000). Smurf2 Is a Ubiquitin E3 Ligase Mediating Proteasome-dependent Degradation of Smad2 in Transforming Growth Factor-β Signaling. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.c000580200
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
