The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Structural Dynamics of Green Fluorescent Protein Alone and Fused with a Single Chain Fv Protein
Open accessAbstract
Structural information on intracellular fusions of the green fluorescent protein (GFP) of the jellyfish Aequorea victoria with endogenous proteins is required as they are increasingly used in cell biology and biochemistry. We have investigated the dynamic properties of GFP alone and fused to a single chain antibody raised against lipopolysaccharide of the outer cell wall of gram-negative bacteria (abbreviated as scFv-GFP). The scFv moiety was functional as was proven in binding assays, which involved the use of both fluorescence correlation spectroscopy observing the binding of scFv-GFP to gram-negative bacteria and a surface plasmon resonance cell containing adsorbed lipopolysaccharide antigen. The rotational motion of scFv-GFP has been investigated with time-resolved fluorescence anisotropy. However, the rotational correlation time of scFv-GFP is too short to account for globular rotation of the whole protein. This result can only be explained by assuming a fast hinge motion between the two fused proteins. A modeled structure of scFv-GFP supports this observation.
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Cites
PROCHECK: a program to check the stereochemical quality of protein structures
Laskowski, R. A., MacArthur, M. W., Moss, D. S., & Thornton, J. M. (1993) · Journal of Applied Crystallography · doi:10.1107/s0021889892009944 · Cited by 24,581
Cited by
Nordin, J. Z., Lee, Y., Vader, P., Mäger, I., Johansson, H. J., Heusermann, W., Wiklander, O. P. B., Hällbrink, M., Seow, Y., Bultema, J. J., et al. (2015) · Nanomedicine: Nanotechnology, Biology and Medicine · doi:10.1016/j.nano.2015.01.003 · Cited by 678
Biosynthesis and Metabolism of Cyclopropane Rings in Natural Compounds
Wessjohann, L. A., Brandt, W., & Thiemann, T. (2003) · Chemical Reviews · doi:10.1021/cr0100188 · Cited by 640
Mapping Intracellular Temperature Using Green Fluorescent Protein
Donner, J. S., Thompson, S. A., Kreuzer, M. P., Baffou, G., & Quidant, R. (2012) · Nano Letters · doi:10.1021/nl300389y · Cited by 441
Ultra-stable organic fluorophores for single-molecule research
Zheng, Q., Juette, M. F., Jockusch, S., Wasserman, M. R., Zhou, Z., Altman, R. B., & Blanchard, S. C. (2014) · Chem. Soc. Rev. · doi:10.1039/c3cs60237k · Cited by 406
Gaetani, M. L., Pinto, I. C., Li, M., O’Connor, P., Giorgi-Coll, S., Tyreman, M., Rumary, K. L., Schouten, J. A., Davis, P., & Dixon, A. M. (2024) · Molecular Immunology · doi:10.1016/j.molimm.2023.12.008 · Cited by 3
Metadata
- Authors
- Hink, M. A., Griep, R. A., Borst, J. W., van Hoek, A., Eppink, M. H. M., Schots, A., & Visser, A. J. W. G.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2000-06
- Volume
- 275
- Issue
- 23
- Pages
- 17556-17560
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1999429527
- Cited by
- 216
- References
- 1
- FWCI
- 5.96
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Hink, M. A., Griep, R. A., Borst, J. W., van Hoek, A., Eppink, M. H. M., Schots, A., & Visser, A. J. W. G. (2000). Structural Dynamics of Green Fluorescent Protein Alone and Fused with a Single Chain Fv Protein. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m001348200
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