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Cited by 678

The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.

Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties

Open access
Nordin, Joel Z., Lee, Yi, Vader, Pieter, Mäger, Imre, Johansson, Henrik J., Heusermann, Wolf, Wiklander, Oscar P.B., Hällbrink, Mattias, Seow, Yiqi, Bultema, Jarred J., Gilthorpe, Jonathan, Davies, Tim, Fairchild, Paul J., Gabrielsson, Susanne, Meisner-Kober, Nicole C., Lehtiö, Janne, Smith, C.I. Edvard, Wood, Matthew J.A., Andaloussi, Samir EL·2015·Nanomedicine: Nanotechnology, Biology and Medicine
DOI 10.1016/j.nano.2015.01.003
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Abstract

Extracellular vesicles (EVs) are natural nanoparticles that mediate intercellular transfer of RNA and proteins and are of great medical interest; serving as novel biomarkers and potential therapeutic agents. However, there is little consensus on the most appropriate method to isolate high-yield and high-purity EVs from various biological fluids. Here, we describe a systematic comparison between two protocols for EV purification: ultrafiltration with subsequent liquid chromatography (UF-LC) and differential ultracentrifugation (UC). A significantly higher EV yield resulted from UF-LC as compared to UC, without affecting vesicle protein composition. Importantly, we provide novel evidence that, in contrast to UC-purified EVs, the biophysical properties of UF-LC-purified EVs are preserved, leading to a different in vivo biodistribution, with less accumulation in lungs. Finally, we show that UF-LC is scalable and adaptable for EV isolation from complex media types such as stem cell media, which is of huge significance for future clinical applications involving EVs. FROM THE CLINICAL EDITOR: Recent evidence suggests extracellular vesicles (EVs) as another route of cellular communication. These EVs may be utilized for future therapeutics. In this article, the authors compared ultrafiltration with size-exclusion liquid chromatography (UF-LC) and ultra-centrifugation (UC) for EV recovery.

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Metadata

Authors
Nordin, J. Z., Lee, Y., Vader, P., Mäger, I., Johansson, H. J., Heusermann, W., Wiklander, O. P. B., Hällbrink, M., Seow, Y., Bultema, J. J., Gilthorpe, J., Davies, T., Fairchild, P. J., Gabrielsson, S., Meisner-Kober, N. C., Lehtiö, J., Smith, C. I. E., Wood, M. J. A., & Andaloussi, S. E.
Venue
Nanomedicine: Nanotechnology, Biology and Medicine
Publisher
Elsevier BV
Published
2015-05
Volume
11
Issue
4
Pages
879-883
ISSN
1549-9634 (ISSN-L)
DOI
10.1016/j.nano.2015.01.003
OpenAlex
W2144026504
Cited by
678
References
19
FWCI
17.75
Type
journal article
Language
en
Open access
hybrid

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The paper
Nordin, J. Z., Lee, Y., Vader, P., Mäger, I., Johansson, H. J., Heusermann, W., Wiklander, O. P. B., Hällbrink, M., Seow, Y., Bultema, J. J., Gilthorpe, J., Davies, T., Fairchild, P. J., Gabrielsson, S., Meisner-Kober, N. C., Lehtiö, J., Smith, C. I. E., Wood, M. J. A., & Andaloussi, S. E. (2015). Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties. Nanomedicine: Nanotechnology, Biology and Medicine. https://doi.org/10.1016/j.nano.2015.01.003

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.