The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
Molecular Cloning and Functional Characterization of a Novel Mammalian Sphingosine Kinase Type 2 Isoform
Open accessAbstract
Sphingosine-1-phosphate (SPP) has diverse biological functions acting inside cells as a second messenger to regulate proliferation and survival, and extracellularly, as a ligand for G protein-coupled receptors of the endothelial differentiation gene-1 subfamily. Based on sequence homology to murine and human sphingosine kinase-1 (SPHK1), which we recently cloned (Kohama, T., Oliver, A., Edsall, L. , Nagiec, M. M., Dickson, R., and Spiegel, S. (1998) J. Biol. Chem. 273, 23722-23728), we have now cloned a second type of mouse and human sphingosine kinase (mSPHK2 and hSPHK2). mSPHK2 and hSPHK2 encode proteins of 617 and 618 amino acids, respectively, both much larger than SPHK1, and though diverging considerably, both contain the conserved domains found in all SPHK1s. Northern blot analysis revealed that SPHK2 mRNA expression had a strikingly different tissue distribution from that of SPHK1 and appeared later in embryonic development. Expression of SPHK2 in HEK 293 cells resulted in elevated SPP levels. d-erythro-dihydrosphingosine was a better substrate than d-erythro-sphingosine for SPHK2. Surprisingly, d, l-threo-dihydrosphingosine was also phosphorylated by SPHK2. In contrast to the inhibitory effects on SPHK1, high salt concentrations markedly stimulated SPHK2. Triton X-100 inhibited SPHK2 and stimulated SPHK1, whereas phosphatidylserine stimulated both type 1 and type 2 SPHK. Thus, SPHK2 is another member of a growing class of sphingolipid kinases that may have novel functions.
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Cites
Dudley, R. K. (1988) · FEBS Letters · doi:10.1016/0014-5793(88)80264-3 · Cited by 5,945
Suppression of ceramide-mediated programmed cell death by sphingosine-1-phosphate
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Sphingosine-1-Phosphate as a Ligand for the G Protein-Coupled Receptor EDG-1
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Self-citationSphingosine-1-phosphate as second messenger in cell proliferation induced by PDGF and FCS mitogens
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Self-citationMolecular Cloning and Functional Characterization of Murine Sphingosine Kinase
Kohama, T., Olivera, A., Edsall, L., Nagiec, M. M., Dickson, R., & Spiegel, S. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.37.23722 · Cited by 535
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Van Brocklyn, J. R., Lee, M.-J., Menzeleev, R., Olivera, A., Edsall, L., Cuvillier, O., Thomas, D. M., Coopman, P. J. P., Thangada, S., Liu, C. H., et al. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.142.1.229 · Cited by 460
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Cited by
Regulation of histone acetylation in the nucleus by sphingosine-1-phosphate
Hait, N. C., Allegood, J., Maceyka, M., Strub, G. M., Harikumar, K. B., Singh, S. K., Luo, C., Marmorstein, R., Kordula, T., Milstien, S., et al. (2009) · Science · doi:10.1126/science.1176709 · Cited by 997
Self-citationSphingosine 1-phosphate and cancer
Pyne, N. J., & Pyne, S. (2010) · Nature Reviews Cancer · doi:10.1038/nrc2875 · Cited by 852
Sphingosine 1-Phosphate, a Key Cell Signaling Molecule
Spiegel, S., & Milstien, S. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.r200007200 · Cited by 555
Self-citationAn update on the biology of sphingosine 1-phosphate receptors
Blaho, V. A., & Hla, T. (2014) · Journal of Lipid Research · doi:10.1194/jlr.r046300 · Cited by 516
Phosphorylation of the immunomodulatory drug FTY720 by sphingosine kinases
Billich, A., Bornancin, F., Dévay, P., Mechtcheriakova, D., Urtz, N., & Baumruker, T. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m307687200 · Cited by 433
Metadata
- Authors
- Liu, H., Sugiura, M., Nava, V. E., Edsall, L. C., Kono, K., Poulton, S., Milstien, S., Kohama, T., & Spiegel, S.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2000-06
- Volume
- 275
- Issue
- 26
- Pages
- 19513-19520
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1993449698
- Cited by
- 667
- References
- 40
- FWCI
- 6.53
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Liu, H., Sugiura, M., Nava, V. E., Edsall, L. C., Kono, K., Poulton, S., Milstien, S., Kohama, T., & Spiegel, S. (2000). Molecular Cloning and Functional Characterization of a Novel Mammalian Sphingosine Kinase Type 2 Isoform. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m002759200
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