The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.
C-Mannosylation and O-Fucosylation of the Thrombospondin Type 1 Module
Open accessAbstract
Thrombospondin-1 (TSP-1) is a multidomain protein that has been implicated in cell adhesion, motility, and growth. Some of these functions have been localized to the three thrombospondin type 1 repeats (TSRs), modules of approximately 60 amino acids in length with conserved Cys and Trp residues. The Trp residues occur in WXXW patterns, which are the recognition motifs for protein C-mannosylation. This modification involves the attachment of an alpha-mannosyl residue to the C-2 atom of the first tryptophan. Analysis of human platelet TSP-1 revealed that Trp-368, -420, -423, and -480 are C-mannosylated. Mannosylation also occurred in recombinant, baculovirally expressed TSR modules from Sf9 and "High Five" cells, contradictory to earlier reports that such cells do not carry out this reaction. In the course of these studies it was appreciated that the TSRs in TSP-1 undergo a second form of unusual glycosylation. By using a novel mass spectrometric approach, it was found that Ser-377, Thr-432, and Thr-489 in the motif CSX(S/T)CG carry the O-linked disaccharide Glc-Fuc-O-Ser/Thr. This is the first protein in which such a disaccharide has been identified, although protein O-fucosylation is well described in epidermal growth factor-like modules. Both C- and O-glycosylations take place on residues that have been implicated in the interaction of TSP-1 with glycosaminoglycans or other cellular receptors.
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Cites
Signals leading to apoptosis-dependent inhibition of neovascularization by thrombospondin-1
Jiménez, B., Volpert, O. V., Crawford, S. E., Febbraio, M., Silverstein, R. L., & Bouck, N. (2000) · Nature Medicine · doi:10.1038/71517 · Cited by 1,020
Fringe is a glycosyltransferase that modifies Notch
Moloney, D. J., Panin, V. M., Johnston, S. H., Chen, J., Shao, L., Wilson, R., Wang, Y., Stanley, P., Irvine, K. D., Haltiwanger, R. S., et al. (2000) · Nature · doi:10.1038/35019000 · Cited by 852
Glycosyltransferase activity of Fringe modulates Notch–Delta interactions
Brückner, K., Perez, L., Clausen, H., & Cohen, S. (2000) · Nature · doi:10.1038/35019075 · Cited by 694
CD36 Mediates the In Vitro Inhibitory Effects of Thrombospondin-1 on Endothelial Cells
Dawson, D. W., Pearce, S. F. A., Zhong, R., Silverstein, R. L., Frazier, W. A., & Bouck, N. P. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.138.3.707 · Cited by 676
CD36 directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes
Oquendo, P., Hundt, E., Lawler, J., & Seed, B. (1989) · Cell · doi:10.1016/0092-8674(89)90406-6 · Cited by 471
Self-citationIsolation and characterization of a high molecular weight glycoprotein from human blood platelets.
Lawler, J. W., Slayter, H. S., & Coligan, J. E. (1978) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)34336-3 · Cited by 433
Self-citationRegulation of Transforming Growth Factor-β Activation by Discrete Sequences of Thrombospondin 1
Schultz-Cherry, S., Chen, H., Mosher, D. F., Misenheimer, T. M., Krutzsch, H. C., Roberts, D. D., & Murphy-Ullrich, J. E. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.13.7304 · Cited by 424
Self-citationInsect cells as hosts for the expression of recombinant glycoproteins
Altmann, F., Staudacher, E., Wilson, I. B. H., & März, L. (1999) · Glycoconjugate Journal · doi:10.1023/a:1026488408951 · Cited by 344
Moloney, D. J., Shair, L. H., Lu, F. M., Xia, J., Locke, R., Matta, K. L., & Haltiwanger, R. S. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.13.9604 · Cited by 336
Adams, J. C., & Tucker, R. P. (2000) · Developmental Dynamics · doi:10.1002/(sici)1097-0177(200006)218:2<280::aid-dvdy4>3.0.co;2-0 · Cited by 314
Cited by
Varki, A. (2017) · Glycobiology · doi:10.1093/glycob/cww086 · Cited by 2,635
“To cite just a few examples, the following glycans were mostly unknown when the previous version of this review was being written: functional sialylation in the fly nervous system (181); O-fucose and O-glucose glycans on Notch (182–184), and O-fucose on thrombospondin repeats (185); mucin type O-glycosylation in proti…”
BackgroundFucose: biosynthesis and biological function in mammals
Becker, D. J., & Lowe, J. B. (2003) · Glycobiology · doi:10.1093/glycob/cwg054 · Cited by 930
Role of Glycosylation in Development
Haltiwanger, R. S., & Lowe, J. B. (2004) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.73.011303.074043 · Cited by 757
Apte, S. S. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.r109.052340 · Cited by 506
Thrombospondins: Multifunctional Regulators of Cell Interactions
Adams, J. C. (2001) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.17.1.25 · Cited by 406
Metadata
- Authors
- Hofsteenge, J., Huwiler, K. G., Macek, B., Hess, D., Lawler, J., Mosher, D. F., & Peter-Katalinic, J.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2001-03
- Volume
- 276
- Issue
- 9
- Pages
- 6485-6498
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2050429474
- Cited by
- 260
- References
- 51
- FWCI
- 4.78
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Hofsteenge, J., Huwiler, K. G., Macek, B., Hess, D., Lawler, J., Mosher, D. F., & Peter-Katalinic, J. (2001). C-Mannosylation and O-Fucosylation of the Thrombospondin Type 1 Module. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m008073200
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