The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Regulation of a Novel Human Phospholipase C, PLCε, through Membrane Targeting by Ras
Open accessAbstract
Phosphoinositide-specific phospholipase C (PI-PLC) plays a pivotal role in regulation of intracellular signal transduction from various receptor molecules. More than 10 members of human PI-PLC isoforms have been identified and classified into three classes beta, gamma, and delta, which are regulated by distinct mechanisms. Here we report identification of a novel class of human PI-PLC, named PLCepsilon, which is characterized by the presence of a Ras-associating domain at its C terminus and a CDC25-like domain at its N terminus. The Ras-associating domain of PLCepsilon specifically binds to the GTP-bound forms of Ha-Ras and Rap1A. The dissociation constant for Ha-Ras is estimated to be approximately 40 nm, comparable with those of other Ras effectors. Co-expression of an activated Ha-Ras mutant with PLCepsilon induces its translocation from the cytosol to the plasma membrane. Upon stimulation with epidermal growth factor, similar translocation of ectopically expressed PLCepsilon is observed, which is inhibited by co-expression of dominant-negative Ha-Ras. Furthermore, using a liposome-based reconstitution assay, it is shown that the phosphatidylinositol 4,5-bisphosphate-hydrolyzing activity of PLCepsilon is stimulated in vitro by Ha-Ras in a GTP-dependent manner. These results indicate that Ras directly regulates phosphoinositide breakdown through membrane targeting of PLCepsilon.
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Metadata
- Authors
- Song, C., Hu, C.-D., Masago, M., Kariya, K.-I., Yamawaki-Kataoka, Y., Shibatohge, M., Wu, D., Satoh, T., & Kataoka, T.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2001-01
- Volume
- 276
- Issue
- 4
- Pages
- 2752-2757
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2073816668
- Cited by
- 320
- References
- 39
- FWCI
- 9.01
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Song, C., Hu, C.-D., Masago, M., Kariya, K.-I., Yamawaki-Kataoka, Y., Shibatohge, M., Wu, D., Satoh, T., & Kataoka, T. (2001). Regulation of a Novel Human Phospholipase C, PLCε, through Membrane Targeting by Ras. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m008324200
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
