The DOI matches its Crossref record; 61 of the 61 references it cites were found in a registry or an index.
Erk Is Essential for Growth, Differentiation, Integrin Expression, and Cell Function in Human Osteoblastic Cells
Open accessAbstract
Extracellular signal-regulated kinases (Erks), members of the mitogen-activated protein kinase superfamily, play an important role in cell proliferation and differentiation. In this study we employed a dominant negative approach to determine the role of Erks in the regulation of human osteoblastic cell function. Human osteoblastic cells were transduced with a pseudotyped retrovirus encoding either a mutated Erk1 protein with a dominant negative action against both Erk1 and Erk2 (Erk1DN cells) or the LacZ protein (LacZ cells) as a control. Both basal and growth factor-stimulated MAPK activity and cell proliferation were inhibited in Erk1DN cells. Expression of Erk1DN protein suppressed both osteoblast differentiation and matrix mineralization by decreasing alkaline phosphatase activity and the deposition of bone matrix proteins. Cell adhesion to collagen, osteopontin, and vitronectin was decreased in Erk1DN cells as compared with LacZ cells. Cell spreading and migration on these matrices were also inhibited. In Erk1DN cells, expression of alphabeta(1), alpha(v)beta(3), and alpha(v)beta(5) integrins on the surface was decreased. Metabolic labeling indicated that the synthesis of these integrins was inhibited in Erk1DN cells. These data suggest that Erks are not only essential for the growth and differentiation of osteoblasts but also are important for osteoblast adhesion, spreading, migration, and integrin expression.
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MAP kinases and cell migration
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Growth Hormone, Insulin-Like Growth Factors, and the Skeleton
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Mechanism of Divergent Growth Factor Effects in Mesenchymal Stem Cell Differentiation
Kratchmarova, I., Blagoev, B., Haack-Sorensen, M., Kassem, M., & Mann, M. (2005) · Science · doi:10.1126/science.1107627 · Cited by 565
Oxidative stress inhibits osteoblastic differentiation of bone cells by ERK and NF-κB
Bai, X.-C., Lu, D., Bai, J., Zheng, H., Ke, Z.-Y., Li, X.-M., & Luo, S.-Q. (2004) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2003.12.073 · Cited by 561
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Metadata
- Authors
- Lai, C.-F., Chaudhary, L., Fausto, A., Halstead, L. R., Ory, D. S., Avioli, L. V., & Cheng, S.-L.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2001-04
- Volume
- 276
- Issue
- 17
- Pages
- 14443-14450
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1537898230
- Cited by
- 381
- References
- 61
- FWCI
- 6.51
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Lai, C.-F., Chaudhary, L., Fausto, A., Halstead, L. R., Ory, D. S., Avioli, L. V., & Cheng, S.-L. (2001). Erk Is Essential for Growth, Differentiation, Integrin Expression, and Cell Function in Human Osteoblastic Cells. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m010021200
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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