Cited by 244Ion Transport and Channel RegulationATP Synthase and ATPases ResearchCellular transport and secretion

Molecular Cloning and Characterization of a Novel (Na+,K+)/H+ Exchanger Localized to the trans-Golgi Network

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Numata, Masayuki, Orlowski, John·2001·Journal of Biological Chemistry

Abstract

The luminal pH of organelles along the secretory and endocytic pathways of mammalian cells is acidic and tightly regulated, with the [H+] varying up to 100-fold between compartments. Steady-state organellar pH is thought to reflect a balance between the rates of H+ pumping by the vacuolar-type H+-ATPase and H+ efflux through ill-defined pathways. Here, we describe the cloning of a novel gene (NHE7) in humans that is homologous to Na+/H+ exchangers, is ubiquitously expressed, and localizes predominantly to the trans-Golgi network. Significantly, NHE7 mediates the influx of Na+ or K+ in exchange for H+. The activity of NHE7 was also found to be relatively insensitive to inhibition by amiloride but could be antagonized by the analogue benzamil and the unrelated compound quinine. Thus, NHE7 displays unique functional and pharmacological properties and may play an important role in maintaining cation homeostasis of this important organelle.

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Authors
Numata, M., & Orlowski, J.
Affiliations
McGill University (CA)
Corresponding author
Orlowski, John
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2001-05
Volume
276
Issue
20
Pages
17387-17394
ISSN
0021-9258 (ISSN-L)
OpenAlex
W2033279834
Cited by
244
References
63
Citations by year
2026: 3 · 2025: 2 · 2024: 3 · 2023: 6 · 2022: 6 · 2021: 5 · 2020: 8 · 2019: 3 · 2018: 5 · 2017: 1 · 2016: 7 · 2015: 6 · 2014: 6 · 2013: 6 · 2012: 7
FWCI
9.54
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • eScholarship@McGill (McGill) (submittedVersion)
  • eScholarship@McGill (McGill) (submittedVersion, cc-by)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › ATP Synthase and ATPases Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
Keywords
Na+/H+ exchanger, trans-Golgi network, cation homeostasis, vacuolar H+-ATPase, benzamil, quinine, gene cloning, secretory pathway
MeSH terms
Amino Acid Sequence, Animals, Carrier Proteins, Cloning, Molecular, Cricetinae, Humans, Hydrogen-Ion Concentration, Models, Molecular, Molecular Sequence Data, Potassium, Recombinant Proteins, Sodium, Transfection, Sequence Alignment, CHO Cells, Sequence Homology, Amino Acid, Protein Structure, Secondary, Antiporters, Sodium-Hydrogen Exchangers, Protein Isoforms, trans-Golgi Network, Membrane Transport Proteins

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