Tissue-specific mRNA expression profiles of human solute carrier transporter superfamilies
Abstract
Pairs of forward and reverse primers and TaqMan probes specific to each of 173 human solute carrier (SLC) transporters were prepared. The mRNA expression level of each target transporter was analyzed in total RNA from single and pooled specimens of various human tissues (adrenal gland, bladder, bone marrow, brain, colon, heart, kidney, liver, lung, mammary gland, ovary, pancreas, peripheral leukocytes, placenta, prostate, retina, salivary gland, skeletal muscle, small intestine, smooth muscle, spinal cord, spleen, stomach, testis, thymus, thyroid gland, trachea, and uterus) by real-time reverse transcription PCR using an Applied Biosystems 7500 Fast Real-Time PCR System. Individual differences in the mRNA expression of human SLC transporters in the liver were also evaluated. These newly determined expression profiles were used to study the gene expression in the 28 different human tissues listed above, and tissues with high transcriptional activity for human SLC transporters were identified. These results are expected to be valuable for research concerning the clinical diagnosis of disease.
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One mechanism for increased L-T4 requirements with tyrosine kinase inhibitors can be inhibition of MCT8-dependent T3 and T4 uptake, since MCT8 is expressed by the gastrointestinal tract.
“One mechanism for the L-T4 increased requirements, further to T4 increased metabolism, can be inhibition of MCT8-dependent T3 and T4 uptake (64), since MCT8 is expressed by the gastrointestinal tract (65).”
From L-T4 therapy in the presence of pharmacological interferents (2020) · cites this paper as [65]
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“One mechanism for the L-T4 increased requirements, further to T4 increased metabolism, can be inhibition of MCT8-dependent T3 and T4 uptake (64), since MCT8 is expressed by the gastrointestinal tract (65).”
Background
- Authors
- Nishimura, M., & Naito, S.
- Affiliations
- Otsuka Pharmaceutical Factory (Japan) (JP)
- Corresponding author
- Naito, Shinsaku
- ORCID
- Nishimura, Masuhiro
- Venue
- Drug Metabolism and Pharmacokinetics
- Published
- 2008
- Volume
- 23
- Issue
- 1
- Pages
- 22-44
- ISSN
- 1347-4367 (ISSN-L)
- OpenAlex
- W2045690571
- Cited by
- 190
- References
- 72
- Citations by year
- 2026: 2 · 2025: 9 · 2024: 6 · 2023: 8 · 2022: 8 · 2021: 13 · 2020: 14 · 2019: 11 · 2018: 14 · 2017: 14 · 2016: 14 · 2015: 12 · 2014: 10 · 2013: 13 · 2012: 10
- FWCI
- 5.63
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Drug Metabolism and Pharmacokinetics (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Pharmacogenetics and Drug Metabolism
- Physical Sciences › Computer Science › Computational Theory and Mathematics › Computational Drug Discovery Methods
- Keywords
- solute carrier transporters, tissue-specific gene expression, mRNA expression profiles, human tissue, transporter gene expression
- MeSH terms
- Gene Expression Regulation, Humans, RNA, Messenger, Solutions, Tissue Distribution, Gene Expression Profiling, Membrane Transport Proteins, Amino Acid Transport Systems
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