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Cited by 307Cellular transport and secretionFungal and yeast genetics researchExtracellular vesicles in disease

Cdc42 Interacts with the Exocyst and Regulates Polarized Secretion

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Zhang, Xiaoyu, Bi, Erfei, Novick, Peter, Du, Lilin, Kozminski, Keith G., Lipschutz, Joshua H., Guo, Wei·2001·Journal of Biological Chemistry
DOI 10.1074/jbc.m107464200
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Abstract

Polarized delivery and incorporation of proteins and lipids to specific domains of the plasma membrane is fundamental to a wide range of biological processes such as neuronal synaptogenesis and epithelial cell polarization. The exocyst complex is specifically localized to sites of active exocytosis and plays essential roles in secretory vesicle targeting and docking at the plasma membrane. Sec3p, a component of the exocyst, is thought to be a spatial landmark for polarized exocytosis. In a search for proteins that regulate the localization of the exocyst in the budding yeast Saccharomyces cerevisiae, we found that certain cdc42 mutants affect the polarized localization of the exocyst proteins. In addition, we found that these mutant cells have a randomized protein secretion pattern on the cell surface. Biochemical experiments indicated that Sec3p directly interacts with Cdc42 in its GTP-bound form. Genetic studies demonstrated synthetically lethal interactions between cdc42 and several exocyst mutants. These results have revealed a role for Cdc42 in exocytosis. We propose that Cdc42 coordinates the vesicle docking machinery and the actin cytoskeleton for polarized secretion.

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  • The subcellular localization of the exocyst in these activated muscles is consistent with the known function of the exocyst in directing post-Golgi vesicles to target membranes for localized membrane addition.

    “The subcellular localization of the exocyst in these activated muscles is consistent with the known function of the exocyst in directing post-Golgi vesicles to target membranes for localized membrane addition”

    From Ral mediates activity‐dependent growth of postsynaptic membranes via recruitment of the exocyst (2013) · cites this paper as Zhang et al, 2001

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Research works printed but not linked: 27

  1. [1]1. Drubin, D.G. ∙ Nelson, W.J. Cell. 1996; 84:335-344 Full Text Full Text (PDF) Scopus (924) PubMed Google Scholar
  2. [2]2. Lew, D.J. ∙ Reed, S.I. Curr. Opin. Genet. Dev. 1995; 5:17-23 Crossref Scopus (198) PubMed Google Scholar
  3. [3]3. Madden, K. ∙ Snyder, M. Annu Rev Microbiol. 1998; 52:687-744 Crossref Scopus (207) PubMed Google Scholar
  4. [4]4. Chant, J. Annu. Rev. Cell Dev. Biol. 1999; 15:365-391 Crossref Scopus (197) PubMed Google Scholar
  5. [5]5. Finger, F.P. ∙ Novick, P.J. J. Cell Biol. 1998; 142:609-612 Crossref Scopus (126) PubMed Google Scholar
  6. [6]6. TerBush, D.R. ∙ Novick, P. J. Cell Biol. 1995; 130:299-312 Crossref Scopus (258) PubMed Google Scholar
  7. [7]7. TerBush, D.R. ∙ Maurice, T.M. ∙ Roth, D. ... EMBO J. 1996; 15:6483-6494 Crossref Scopus (702) PubMed Google Scholar
  8. [8]8. Finger, F.P. ∙ Hughes, T.E. ∙ Novick, P. Cell. 1998; 92:559-571 Full Text Full Text (PDF) Scopus (319) PubMed Google Scholar
  9. [9]9. Hsu, S.C. ∙ Ting, A.E. ∙ Hazuka, C.D. ... Neuron. 1996; 17:1209-1219 Full Text Full Text (PDF) Scopus (231) PubMed Google Scholar
  10. [10]10. Guo, W. ∙ Roth, D. ∙ Walch-Solimena, C. ... EMBO J. 1999; 18:1071-1080 Crossref Scopus (513) PubMed Google Scholar
  11. [11]11. Guo, W. ∙ Grant, A. ∙ Novick, P. J. Biol. Chem. 1999; 274:23558-23564 Full Text Full Text (PDF) Scopus (154) PubMed Google Scholar
  12. [12]12. Pruyne, D.W. ∙ Schott, D.H. ∙ Bretscher, A. J. Cell Biol. 1998; 143:1931-1945 Crossref Scopus (286) PubMed Google Scholar
  13. [13]13. Hazuka, C.D. ∙ Foletti, D.L. ∙ Hsu, S.C. ... J. Neurosci. 1999; 19:1324-1334 Crossref PubMed Google Scholar
  14. [14]14. Grindstaff, K.K. ∙ Yeaman, C. ∙ Anandasabapathy, N. ... Cell. 1998; 93:731-740 Full Text Full Text (PDF) Scopus (454) PubMed Google Scholar
  15. [15]15. Erickson, J.W. ∙ Cerione, R.A. Curr. Opin. Cell Biol. 2001; 13:153-157 Crossref Scopus (152) PubMed Google Scholar
  16. [16]16. Johnson, D.I. Microbiol. Mol. Biol. Rev. 1999; 63:54-105 Crossref PubMed Google Scholar
  17. [17]17. Cabib, E. ∙ Drgonova, J. ∙ Drgon, T. Annu. Rev. Biochem. 1998; 67:307-333 Crossref Scopus (157) PubMed Google Scholar
  18. [18]18. Guo, W. ∙ Tamanoi, F. ∙ Novick, P. Nat. Cell Biol. 2001; 3:353-360 Crossref Scopus (257) PubMed Google Scholar
  19. [19]19. Chant, J. ∙ Stowers, L. Cell. 1995; 7:1-4 Full Text (PDF) Scopus (265) Google Scholar
  20. [20]20. Brown, J.L. ∙ Jaquenoud, M. ∙ Gulli, M.P. ... Genes Dev. 1997; 15:2972-2982 Crossref Scopus (142) Google Scholar
  21. [21]21. Chen, G.C. ∙ Kim, Y.J. ∙ Chan, C.S. Genes Dev. 1997; 15:2958-2971 Crossref Scopus (107) Google Scholar
  22. [22]22. Kozminski, K.G. ∙ Chen, A.J. ∙ Rodal, A.A. ... Mol. Biol. Cell. 2000; 11:339-354 Crossref Scopus (71) PubMed Google Scholar
  23. [23]23. Moskow, J.J. ∙ Gladfelter, A.S. ∙ Lamson, R.E. ... Mol. Cell. Biol. 2000; 20:7559-7571 Crossref Scopus (65) PubMed Google Scholar
  24. [24]24. Gladfelter, A.S. ∙ Moskow, J.J. ∙ Zyla, T.R. ... Mol. Biol. Cell. 2001; 12:1239-1255 Crossref Scopus (46) PubMed Google Scholar
  25. [25]25. Richman, T.J. ∙ Johnson, D.I. Mol. Cell. Biol. 2000; 20:8548-8559 Crossref Scopus (29) PubMed Google Scholar
  26. [26]26. Richman, T.J. ∙ Sawyer, M.M. ∙ Johnson, D.I. J. Biol. Chem. 1999; 274:16861-16870 Full Text Full Text (PDF) Scopus (80) PubMed Google Scholar
  27. [27]27. Novick, P. ∙ Botstein, D. Cell. 1985; 40:405-416

Cited by

Sorted by citations

Authors
Zhang, X., Bi, E., Novick, P., Du, L., Kozminski, K. G., Lipschutz, J. H., & Guo, W.
Affiliations
  • University of Pennsylvania (US)
  • Yale University (US)
  • University of California, Berkeley (US)
Corresponding author
Guo, Wei
ORCID
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2001-12
Volume
276
Issue
50
Pages
46745-46750
ISSN
0021-9258 (ISSN-L)
DOI
10.1074/jbc.m107464200
OpenAlex
W2095506400
Cited by
307
References
0
Citations by year
2026: 2 · 2025: 5 · 2024: 6 · 2023: 2 · 2022: 6 · 2021: 10 · 2020: 12 · 2019: 10 · 2018: 2 · 2017: 9 · 2016: 9 · 2015: 16 · 2014: 11 · 2013: 14 · 2012: 16
FWCI
6.56
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
National Institute of General Medical Sciences (GM 59216, R01GM059216, GM 35370)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
Keywords
Cdc42, exocyst complex, polarized secretion, polarized exocytosis, vesicle docking, actin cytoskeleton, protein-protein interaction, synthetic lethality, Saccharomyces cerevisiae
MeSH terms
Actins, Cell Membrane, Concanavalin A, Cytoskeleton, Exocytosis, Fungal Proteins, Guanosine Triphosphate, Luminescent Proteins, Mutation, Protein Binding, Saccharomyces cerevisiae, Temperature, Time Factors, Protein Structure, Tertiary, cdc42 GTP-Binding Protein, Saccharomyces cerevisiae Proteins, Green Fluorescent Proteins

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