Phosphorylation of the par polarity complex protein Par3 at serine 962 is mediated by aurora a and regulates its function in neuronal polarity.
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The Aurora kinases are a family of serine/threonine protein kinases that perform important functions during the cell cycle. Recently, it was shown that Drosophila Aurora A also regulates the asymmetric localization of Numb to the basal and the partitioning-defective (Par) complex to the apical cortex of neuroblasts by phosphorylating Par6. Here, we show that Aurora A is required for neuronal polarity. Suppression of Aurora A by RNA interference results in the loss of neuronal polarity. Aurora A interacts directly with the atypical protein kinase C binding domain of Par3 and phosphorylates it at serine 962. The phosphorylation of Par3 at serine 962 contributes to its function in the establishment of neuronal polarity.
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The Ndr phosphorylation sites in Par3 (Ser383 and Ser1196) are novel and distinct from the sites for Aurora A, ROCK and Erk2 that regulate Par3 function in neuronal polarity.
“The Ndr phosphorylation sites in Par3 are novel and distinct from the sites for Aurora A, ROCK and Erk2 that regulate its function in neuronal polarity (Chen et al., 2006; Funahashi et al., 2013; Khazaei and Püschel, 2009; Nakayama et al., 2008).”
From Rassf5 and Ndr kinases act in a novel pathway regulating neuronal polarity through Par3 phosphorylation (2014) · cites this paper as Khazaei and Püschel, 2009
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Hippocampal Neuronal Polarity Specified by Spatially Localized mPar3/mPar6 and PI 3-Kinase Activity
Shi, S.-H., Jan, L. Y., & Jan, Y.-N. (2003) · Cell · doi:10.1016/s0092-8674(02)01249-7 · Cited by 600 · see citing works
The sequential activity of the GTPases Rap1B and Cdc42 determines neuronal polarity.
Schwamborn, J. C., & Püschel, A. W. (2004) · Nature Neuroscience · doi:10.1038/nn1295 · Cited by 385 · see citing works
Self-citationFrank, C. L., & Tsai, L.-H. (2009) · Neuron · doi:10.1016/j.neuron.2009.03.029 · Cited by 118 · see citing works
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Integrative proteogenomic characterization of hepatocellular carcinoma across etiologies and stages
Ng, C. K. Y., Dazert, E., Boldanova, T., Coto-Llerena, M., Nuciforo, S., Ercan, C., Suslov, A., Meier, M.-A., Bock, T., Schmidt, A., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-29960-8 · Cited by 165 · see citing works
Yang, R., Kong, E., Jin, J., Hergovich, A., & Püschel, A. W. (2014) · Journal of Cell Science · doi:10.1242/jcs.146696 · Cited by 25 · see citing works
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- Authors
- Khazaei, M. R., & Püschel, A. W.
- Affiliations
- University of Münster (DE)
- Corresponding author
- Püschel, Andreas W.
- ORCID
- Püschel, Andreas W.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2009-11
- Volume
- 284
- Issue
- 48
- Pages
- 33571-33579
- ISSN
- 0021-9258 (ISSN-L)
- PMC
- PMC2785200
- OpenAlex
- W2062126825
- Cited by
- 43
- References
- 3
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 1 · 2022: 5 · 2021: 4 · 2020: 5 · 2019: 2 · 2018: 2 · 2017: 2 · 2016: 1 · 2015: 3 · 2014: 2 · 2013: 3 · 2012: 4
- FWCI
- 1.07
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
- Keywords
- AURKA, Par3, phosphorylation, neuronal polarity, Par complex, neuroblasts, asymmetric localization, Drosophila, cell cycle regulation
- MeSH terms
- Animals, Axons, Binding Sites, Carrier Proteins, Cell Differentiation, Cell Line, Cells, Cultured, Fluorescent Antibody Technique, Humans, Membrane Proteins, Mutation, Nerve Tissue Proteins, Neurons, Phosphorylation, Protein Binding, Serine, Transfection, Blotting, Western, Cell Polarity, Protein Serine-Threonine Kinases, Cell Cycle Proteins, Amino Acid Substitution, RNA Interference, Adaptor Proteins, Signal Transducing, Green Fluorescent Proteins, Rats, Aurora Kinase A, Aurora Kinases
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