The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Matrix GLA Protein, a Regulatory Protein for Bone Morphogenetic Protein-2
Open accessAbstract
Matrix GLA protein (MGP) has been identified as a calcification inhibitor in cartilage and vasculature. Part of this effect may be attributed to its influence on osteoinductive activity of bone morphogenetic protein-2 (BMP-2). To detect binding between MGP and BMP-2, we performed immunoprecipitation using MGP and BMP-2 tagged with FLAG and c-Myc. The results showed co-precipitation of BMP-2 with MGP. To quantify the effect of MGP on BMP-2 activity, we assayed for alkaline phosphatase activity and showed a dose-dependent effect. Low levels of MGP relative to BMP-2 (<1-fold excess) resulted in mild enhancement of osteoinduction, whereas intermediate levels (1-15-fold excess) resulted in strong inhibition. High levels of MGP (>15-fold excess), however, resulted in pronounced enhancement of the osteoinductive effect of BMP-2. Cross-linking studies showed that inhibitory levels of MGP abolished BMP-2 receptor binding. Immunoblotting showed a corresponding decrease in activation of Smad1, part of the BMP signaling system. Enhancing levels of MGP resulted in increased Smad1 activation. To determine the cellular localization of BMP-2 in the presence of MGP, binding assays were performed on whole cells and cell-synthesized matrix. Inhibitory levels of MGP yielded increased matrix binding of BMP-2, suggesting that MGP inhibits BMP-2 in part via matrix association. These results suggest that MGP is a BMP-2 regulatory protein.
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Massagué, J. (1998) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.67.1.753 · Cited by 7,556
Novel Regulators of Bone Formation: Molecular Clones and Activities
Wozney, J. M., Rosen, V., Celeste, A. J., Mitsock, L. M., Whitters, M. J., Kriz, R. W., Hewick, R. M., & Wang, E. A. (1988) · Science · doi:10.1126/science.3201241 · Cited by 3,903
Spontaneous calcification of arteries and cartilage in mice lacking matrix GLA protein
Luo, G., Ducy, P., McKee, M. D., Pinero, G. J., Loyer, E., Behringer, R. R., & Karsenty, G. (1997) · Nature · doi:10.1038/386078a0 · Cited by 2,120
The Spemann Organizer Signal noggin Binds and Inactivates Bone Morphogenetic Protein 4
Zimmerman, L. B., De Jesús-Escobar, J. M., & Harland, R. M. (1996) · Cell · doi:10.1016/s0092-8674(00)80133-6 · Cited by 1,633
Piccolo, S., Sasai, Y., Lu, B., & De Robertis, E. M. (1996) · Cell · doi:10.1016/s0092-8674(00)80132-4 · Cited by 1,126
Bone morphogenetic protein expression in human atherosclerotic lesions.
Boström, K., Watson, K. E., Horn, S., Wortham, C., Herman, I. M., & Demer, L. L. (1993) · Journal of Clinical Investigation · doi:10.1172/jci116391 · Cited by 1,020
Self-citationThe head inducer Cerberus is a multifunctional antagonist of Nodal, BMP and Wnt signals
Piccolo, S., Agius, E., Leyns, L., Bhattacharyya, S., Grunz, H., Bouwmeester, T., & Robertis, E. M. D. (1999) · Nature · doi:10.1038/17820 · Cited by 832
Alternative Neural Crest Cell Fates Are Instructively Promoted by TGFβ Superfamily Members
Shah, N. M., Groves, A. K., & Anderson, D. J. (1996) · Cell · doi:10.1016/s0092-8674(00)81112-5 · Cited by 782
High expression of genes for calcification-regulating proteins in human atherosclerotic plaques.
Shanahan, C. M., Cary, N. R., Metcalfe, J. C., & Weissberg, P. L. (1994) · Journal of Clinical Investigation · doi:10.1172/jci117246 · Cited by 611
Signaling of transforming growth factor‐β family members through Smad proteins
Itoh, S., Itoh, F., Goumans, M., & ten Dijke, P. (2000) · European Journal of Biochemistry · doi:10.1046/j.1432-1327.2000.01828.x · Cited by 529
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Chronic Kidney Disease: Effects on the Cardiovascular System
Schiffrin, E. L., Lipman, M. L., & Mann, J. F. E. (2007) · Circulation · doi:10.1161/circulationaha.106.678342 · Cited by 1,448
Petsophonsakul, P., Furmanik, M., Forsythe, R., Dweck, M., Schurink, G. W., Natour, E., Reutelingsperger, C., Jacobs, M., Mees, B., & Schurgers, L. (2019) · Arteriosclerosis, Thrombosis, and Vascular Biology · doi:10.1161/atvbaha.119.312787 · Cited by 341
Shao, J.-S., Cheng, S.-L., Charlton-Kachigian, N., Loewy, A. P., & Towler, D. A. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m308825200 · Cited by 174
Boström, K., Zebboudj, A. F., Yao, Y., Lin, T. S., & Torres, A. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m406868200 · Cited by 112
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Metadata
- Authors
- Zebboudj, A. F., Imura, M., & Boström, K.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2002-02
- Volume
- 277
- Issue
- 6
- Pages
- 4388-4394
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2130835350
- Cited by
- 357
- References
- 28
- FWCI
- 12.35
- Type
- journal article
- Language
- en
- Open access
- hybrid
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