The DOI matches its Crossref record; 66 of the 66 references it cites were found in a registry or an index.
Liganded Androgen Receptor Interaction with β-Catenin
Open accessAbstract
A yeast two-hybrid assay was employed to identify androgen receptor (AR) protein partners in gonadotropin-releasing hormone neuronal cells. By using an AR deletion construct (AR-(Delta371-485)) as a bait, beta-catenin was identified as an AR-interacting protein from a gonadotropin-releasing hormone neuronal cell library. Immunolocalization of co-transfected AR and FLAG-beta-catenin demonstrated that FLAG-beta-catenin was predominantly cytoplasmic in the absence of androgen. In the presence of 5alpha-dihydrotestosterone, FLAG-beta-catenin completely co-localized to the nucleus with AR. This effect was specific to AR because liganded progesterone, glucocorticoid, or estrogen alpha receptors did not translocate FLAG-beta-catenin to the nucleus. Agonist-bound AR was required because the AR antagonists casodex and hydroxyflutamide failed to translocate beta-catenin. Time course experiments demonstrated that co-translocation occurred with similar kinetics. Nuclear co-localization was independent of the glycogen synthase kinase-3beta, p42/44 ERK mitogen-activated protein kinase, and phosphatidylinositol 3-kinase pathways because inhibitors of these pathways had no effect. Transcription assays demonstrated that liganded AR repressed beta-catenin/T cell factor-responsive reporter gene activity. Conversely, co-expression of beta-catenin/T cell factor repressed AR stimulation of AR-responsive reporter gene activity. Our data suggest that liganded AR shuttles beta-catenin to the nucleus and that nuclear interaction of AR with beta-catenin may modulate transcriptional activity in androgen target tissues.
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“Indeed, ligand-bound AR decreases the transcriptional activity of TCF4 in prostate cancer, colon cancer [21], and neurons [16].”
From The FKBP52 Cochaperone Acts in Synergy with β-Catenin to Potentiate Androgen Receptor Signaling (2015) · cites this paper as [16]
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Cites
Identification of c- MYC as a Target of the APC Pathway
He, T.-C., Sparks, A. B., Rago, C., Hermeking, H., Zawel, L., da Costa, L. T., Morin, P. J., Vogelstein, B., & Kinzler, K. W. (1998) · Science · doi:10.1126/science.281.5382.1509 · Cited by 4,566
Functional interaction of β-catenin with the transcription factor LEF-1
Behrens, J., von Kries, J. P., Kühl, M., Bruhn, L., Wedlich, D., Grosschedl, R., & Birchmeier, W. (1996) · Nature · doi:10.1038/382638a0 · Cited by 2,912
Constitutive Transcriptional Activation by a β-Catenin-Tcf Complex in APC −/− Colon Carcinoma
Korinek, V., Barker, N., Morin, P. J., van Wichen, D., de Weger, R., Kinzler, K. W., Vogelstein, B., & Clevers, H. (1997) · Science · doi:10.1126/science.275.5307.1784 · Cited by 2,905
β‐catenin is a target for the ubiquitin–proteasome pathway
Aberle, H., Bauer, A., Stappert, J., Kispert, A., & Kemler, R. (1997) · The EMBO Journal · doi:10.1093/emboj/16.13.3797 · Cited by 2,586
Transport Between the Cell Nucleus and the Cytoplasm
Görlich, D., & Kutay, U. (1999) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.15.1.607 · Cited by 2,006
XTcf-3 Transcription Factor Mediates β-Catenin-Induced Axis Formation in Xenopus Embryos
Molenaar, M., van de Wetering, M., Oosterwegel, M., Peterson-Maduro, J., Godsave, S., Korinek, V., Roose, J., Destrée, O., & Clevers, H. (1996) · Cell · doi:10.1016/s0092-8674(00)80112-9 · Cited by 1,824
Ozawa, M., Baribault, H., & Kemler, R. (1989) · The EMBO Journal · doi:10.1002/j.1460-2075.1989.tb03563.x · Cited by 1,396
Yost, C., Torres, M., Miller, J., Huang, E., Kimelman, D., & Moon, R. T. (1996) · Genes & Development · doi:10.1101/gad.10.12.1443 · Cited by 1,167
Nongenotropic, Sex-Nonspecific Signaling through the Estrogen or Androgen Receptors
Kousteni, S., Bellido, T., Plotkin, L. I., O’Brien, C. A., Bodenner, D., Li, H., Han, K., Gregorio, G. B. D., Katzenellenbogen, J. A., Katzenellenbogen, B. S., et al. (2001) · Cell · doi:10.1016/s0092-8674(01)00268-9 · Cited by 1,163
Immortalization of hypothalamic GnRH by genetically targeted tumorigenesis
Mellon, P. L., Windle, J. J., Goldsmith, P. C., Padula, C. A., Roberts, J. L., & Weiner, R. I. (1990) · Neuron · doi:10.1016/0896-6273(90)90028-e · Cited by 960
Flagged first, then by citations
Research works printed but not linked: 14
- Kousteni, 2001, Cell., 104, 719
- Roche, 1992, Mol. Endocrinol., 6, 2229
- Adler, 1993, Mol. Cell. Biol., 13, 6326
- Scarlett, 1995, Mol. Endocrinol., 9, 413
- Jorgensen, 2001, Mol. Endocrinol., 15, 1496
- Jorgensen, 2001, Mol. Endocrinol., 15, 1505
- Curtin, 2001, Mol. Endocrinol., 15, 1906
- Issack, 1998, Cell Growth Differ., 9, 837
- Fukuchi, 1998, Cancer Res., 58, 3526
- Garcia-Rostan, 1999, Cancer Res., 59, 1811
- Mirabelli-Primdahl, 1999, Cancer Res., 59, 3346
- Palacios, 1998, Cancer Res., 58, 1344
- Voeller, 1998, Cancer Res., 58, 2520
- Truica, 2000, Cancer Res., 60, 4709
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The many faces and functions of β‐catenin
Valenta, T., Hausmann, G., & Basler, K. (2012) · The EMBO Journal · doi:10.1038/emboj.2012.150 · Cited by 1,598
TCF/LEFs and Wnt Signaling in the NucleusPreprint
Cadigan, K. M., & Waterman, M. L. (2012) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a007906 · Cited by 780
Heemers, H. V., & Tindall, D. J. (2007) · Endocrine Reviews · doi:10.1210/er.2007-0019 · Cited by 753
Sawakami, K., Robling, A. G., Ai, M., Pitner, N. D., Liu, D., Warden, S. J., Li, J., Maye, P. F., Rowe, D. W., Duncan, R. L., et al. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m601000200 · Cited by 399
Interaction of Nuclear Receptors with the Wnt/β-Catenin/Tcf Signaling Axis: Wnt You Like to Know?
Mulholland, D. J., Dedhar, S., Coetzee, G. A., & Nelson, C. C. (2005) · Endocrine Reviews · doi:10.1210/er.2003-0034 · Cited by 377
Metadata
- Authors
- Pawlowski, J. E., Ertel, J. R., Allen, M. P., Xu, M., Butler, C., Wilson, E. M., & Wierman, M. E.
- Affiliations
- Veterans Health Administration (US)
- University of Colorado Health (US)
- University of North Carolina at Chapel Hill (US)
- Corresponding author
- Wierman, Margaret E.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2002-06
- Volume
- 277
- Issue
- 23
- Pages
- 20702-20710
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2033728702
- Cited by
- 166
- References
- 66
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 2 · 2022: 4 · 2021: 4 · 2020: 1 · 2019: 3 · 2018: 5 · 2017: 3 · 2016: 2 · 2015: 5 · 2014: 6 · 2013: 10 · 2012: 4
- FWCI
- 6.26
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Prostate Cancer Treatment and Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Estrogen and related hormone effects
- Keywords
- androgen receptor, β-catenin, protein-protein interaction, nuclear translocation, dihydrotestosterone, GnRH neurons, transcriptional repression, T-cell factor, bicalutamide, hydroxyflutamide, androgen receptor antagonists, transcriptional regulation
- MeSH terms
- Androgen Antagonists, Animals, Cell Line, Cell Nucleus, Cytoskeletal Proteins, Immunohistochemistry, Ligands, Neurons, Precipitin Tests, Promoter Regions, Genetic, Receptors, Androgen, Receptors, Estrogen, Receptors, Glucocorticoid, Receptors, Progesterone, Transcription, Genetic, Blotting, Western, Trans-Activators, COS Cells, Two-Hybrid System Techniques, Protein Transport, Estrogen Receptor alpha, beta Catenin, Mice
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