Cited by 127Protein Kinase Regulation and GTPase SignalingEscherichia coli research studiesToxin Mechanisms and Immunotoxins

37-kDa Laminin Receptor Precursor Modulates Cytotoxic Necrotizing Factor 1–mediated RhoA Activation and Bacterial Uptake

Open access
Chung, Jin Woong, Hong, Suk Jin, Kim, Kee Jun, Goti, Daniel, Stins, Monique F., Shin, Sooan, Dawson, Valina L., Dawson, Ted M. +1 more·2003·Journal of Biological Chemistry

Abstract

Cytotoxic necrotizing factor 1 (CNF1) is a bacterial toxin known to activate Rho GTPases and induce host cell cytoskeleton rearrangements. The constitutive activation of Rho GTPases by CNF1 is shown to enhance bacterial uptake in epithelial cells and human brain microvascular endothelial cells. However, it is unknown how exogenous CNF1 exhibits such phenotypes in eukaryotic cells. Here, we identified 37-kDa laminin receptor precursor (LRP) as the receptor for CNF1 from screening the cDNA library of human brain microvascular endothelial cells by the yeast two-hybrid system using the N-terminal domain of CNF1 as bait. CNF1-mediated RhoA activation and bacterial uptake were inhibited by exogenous LRP or LRP antisense oligodeoxynucleotides, whereas they were increased in LRP-overexpressing cells. These findings indicate that the CNF1 interaction with LRP is the initial step required for CNF1-mediated RhoA activation and bacterial uptake in eukaryotic cells.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1074/jbc.m301028200

Cites

39 of 39 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Other meningeal pathogens bind ECM components and receptors to mediate bacterial-BBB interactions.

    “Other meningeal pathogens, such as Streptococcus pneumoniae (SPN), E. coli K1, Neisseria meningitidis, and Haemophilus influenza type B (HiB, also bind ECM components and ECM receptors (e.g., integrins and laminin receptor) to mediate bacterial-BBB interactions (12)(13)(14)(15).”

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Chung, J. W., Hong, S. J., Kim, K. J., Goti, D., Stins, M. F., Shin, S., Dawson, V. L., Dawson, T. M., & Kim, K. S.
Affiliations
Johns Hopkins University (US)
Corresponding author
Kim, Kwang Sik
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2003-05
Volume
278
Issue
19
Pages
16857-16862
ISSN
0021-9258 (ISSN-L)
OpenAlex
W2039475868
Cited by
127
References
38
Citations by year
2026: 1 · 2025: 3 · 2024: 2 · 2023: 4 · 2022: 3 · 2021: 16 · 2020: 2 · 2019: 2 · 2018: 6 · 2017: 2 · 2016: 4 · 2015: 8 · 2014: 8 · 2013: 5 · 2012: 6
FWCI
3.34
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
National Institutes of Health
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
  • Life Sciences › Immunology and Microbiology › Immunology › Toxin Mechanisms and Immunotoxins
Keywords
cytotoxic necrotizing factor 1, RhoA activation, bacterial internalization, Rho GTPases, toxin receptor, brain microvascular endothelial cells, cytoskeleton rearrangement, host-pathogen interaction, antisense oligodeoxynucleotides
MeSH terms
Bacterial Toxins, Cell Line, Cytotoxins, Endothelium, Vascular, Enzyme Activation, Escherichia coli, Escherichia coli Infections, Protein Binding, Protein Precursors, Signal Transduction, Oligonucleotides, Antisense, Receptors, Laminin, rhoA GTP-Binding Protein, Escherichia coli Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review