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Cited by 834

The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.

Exosome Release Is Regulated by a Calcium-dependent Mechanism in K562 Cells

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Savina, Ariel, Furlán, Marcelo, Vidal, Michel, Colombo, Maria I.·2003·Journal of Biological Chemistry
DOI 10.1074/jbc.m301642200
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Abstract

Multivesicular bodies (MVBs) are endocytic structures that contain small vesicles formed by the budding of an endosomal membrane into the lumen of the compartment. Fusion of MVBs with the plasma membrane results in secretion of the small internal vesicles termed exosomes. K562 cells are a hematopoietic cell line that releases exosomes. The application of monensin (MON) generated large MVBs that were labeled with a fluorescent lipid. Exosome release was markedly enhanced by MON treatment, a Na+/H+ exchanger that induces changes in intracellular calcium (Ca2+). To explore the possibility that the effect of MON on exosome release was caused via an increase in Ca2+, we have used a calcium ionophore and a chelator of intracellular Ca2+. Our results indicate that increasing intracellular Ca2+ stimulates exosome secretion. Furthermore, MON-stimulated exosome release was completely eliminated by 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid acetoxymethyl ester (BAPTA-AM), implying a requirement for Ca2+ in this process. We have observed that the large MVBs generated in the presence of MON accumulated Ca2+ as determined by labeling with Fluo3-AM, suggesting that intralumenal Ca2+ might play a critical role in the secretory process. Interestingly, our results indicate that transferrin (Tf) stimulated exosome release in a Ca2+-dependent manner, suggesting that Tf might be a physiological stimulus for exosome release in K562 cells.

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Metadata

Authors
Savina, A., Furlán, M., Vidal, M., & Colombo, M. I.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2003-05
Volume
278
Issue
22
Pages
20083-20090
ISSN
0021-9258 (ISSN-L)
DOI
10.1074/jbc.m301642200
OpenAlex
W2055791162
Cited by
834
References
45
FWCI
2.57
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Exosome Release Is Regulated by a Calcium-dependent Mechanism in K562 Cells. https://app.cochat.ai/papers/10.1074/jbc.m301642200
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Savina, A., Furlán, M., Vidal, M., & Colombo, M. I. (2003). Exosome Release Is Regulated by a Calcium-dependent Mechanism in K562 Cells. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m301642200

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