The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
Pulmonary Endothelial Cell Barrier Enhancement by Sphingosine 1-Phosphate
Open accessAbstract
We recently reported the critical importance of Rac GTPase-dependent cortical actin rearrangement in the augmentation of pulmonary endothelial cell (EC) barrier function by sphingosine 1-phosphate (S1P). We now describe functional roles for the actin-binding proteins cortactin and EC myosin light chain kinase (MLCK) in mediating this response. Antisense down-regulation of cortactin protein expression significantly inhibits S1P-induced barrier enhancement in cultured human pulmonary artery EC as measured by transendothelial electrical resistance (TER). Immunofluorescence studies reveal rapid, Rac-dependent translocation of cortactin to the expanded cortical actin band following S1P challenge, where colocalization with EC MLCK occurs within 5 min. Adenoviral overexpression of a Rac dominant negative mutant attenuates TER elevation by S1P. S1P also induces a rapid increase in cortactin tyrosine phosphorylation (within 30 s) critical to subsequent barrier enhancement, since EC transfected with a tyrosine-deficient mutant cortactin exhibit a blunted TER response. Direct binding of EC MLCK to the cortactin Src homology 3 domain appears essential to S1P barrier regulation, since cortactin blocking peptide inhibits both S1P-induced MLC phosphorylation and peak S1P-induced TER values. These data support novel roles for the cytoskeletal proteins cortactin and EC MLCK in mediating lung vascular barrier augmentation evoked by S1P.
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Cites
Alteration of Lymphocyte Trafficking by Sphingosine-1-Phosphate Receptor Agonists
Mandala, S., Hajdu, R., Bergstrom, J., Quackenbush, E., Xie, J., Milligan, J., Thornton, R., Shei, G.-J., Card, D., Keohane, C., et al. (2002) · Science · doi:10.1126/science.1070238 · Cited by 1,690
Cytoskeletal regulation of pulmonary vascular permeability
Dudek, S. M., & Garcia, J. G. N. (2001) · Journal of Applied Physiology · doi:10.1152/jappl.2001.91.4.1487 · Cited by 985
Self-citationGarcia, J. G. N., Liu, F., Verin, A. D., Birukova, A., Dechert, M. A., Gerthoffer, W. T., Bamberg, J. R., & English, D. (2001) · Journal of Clinical Investigation · doi:10.1172/jci12450 · Cited by 886
Self-citationCortactin promotes and stabilizes Arp2/3-induced actin filament network formation
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Dedicated Myosin Light Chain Kinases with Diverse Cellular Functions
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Activation of Arp2/3 complex-mediated actin polymerization by cortactin
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Self-citationGarcia, J. G. N., Davis, H. W., & Patterson, C. E. (1995) · Journal of Cellular Physiology · doi:10.1002/jcp.1041630311 · Cited by 547
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Identification and characterization of a novel cytoskeleton-associated pp60src substrate.
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Cortactin: coupling membrane dynamics to cortical actin assembly
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Birukova, A. A., Zagranichnaya, T., Fu, P., Alekseeva, E., Chen, W., Jacobson, J. R., & Birukov, K. G. (2007) · Experimental Cell Research · doi:10.1016/j.yexcr.2007.03.036 · Cited by 285
Self-citationCamp, S. M., Ceco, E., Evenoski, C. L., Danilov, S. M., Zhou, T., Chiang, E. T., Moreno-Vinasco, L., Mapes, B., Zhao, J., Gursoy, G., et al. (2015) · Scientific Reports · doi:10.1038/srep13135 · Cited by 180
Self-citationSu, G., Atakilit, A., Li, J. T., Wu, N., Bhattacharya, M., Zhu, J., Shieh, J. E., Li, E., Chen, R., Sun, S., et al. (2012) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.201108-1381oc · Cited by 92
Quijada, H., Bermudez, T., Kempf, C. L., Valera, D. G., Garcia, A. N., Camp, S. M., Song, J. H., Franco, E., Burt, J. K., Sun, B., et al. (2021) · European Respiratory Journal · doi:10.1183/13993003.02536-2020 · Cited by 88
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Metadata
- Authors
- Dudek, S. M., Jacobson, J. R., Chiang, E. T., Birukov, K. G., Wang, P., Zhan, X., & Garcia, J. G. N.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2004-06
- Volume
- 279
- Issue
- 23
- Pages
- 24692-24700
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2016189603
- Cited by
- 301
- References
- 41
- FWCI
- 6.34
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Dudek, S. M., Jacobson, J. R., Chiang, E. T., Birukov, K. G., Wang, P., Zhan, X., & Garcia, J. G. N. (2004). Pulmonary Endothelial Cell Barrier Enhancement by Sphingosine 1-Phosphate. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m313969200
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