Chromatin-level Regulation of the IL10 Gene in T Cells
Open accessAbstract
The immunoregulatory cytokine interleukin 10 (IL-10) modulates the function of diverse immune and non-immune cells. Here, we examine the chromatin structural changes associated with IL10 gene transcription by naive and differentiated murine T cells. Naive T cells lack DNase I hypersensitive (HS) sites in the vicinity of the IL10 gene, whereas differentiated T cells display a strong 3' constitutive HS site as well as several inducible sites. The majority of HS sites map to regions that are strongly conserved in sequence between mouse and human genomes. In committed Th1 cells, the mechanism of IL10 gene silencing is associated with the development of repressive histone modifications near the IL10 promoter and also near intronic hypersensitive regions of the IL10 gene. Our results constitute the first report of chromatin structural differences within the IL10 gene in differentiated Th1 and Th2 cells and emphasize the surprising diversity of mechanisms used to regulate cytokine gene expression at the chromatin level.
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Dynamic changes in the chromatin structure of the Il10 promoter in T cells differentiated into the TH1 or TH2 phenotype closely regulate IL-10 expression.
“Dynamic changes in the chromatin structure of the Il10 promoter in T cells differentiated into the TH1 or TH2 phenotype closely regulate IL-10 expression32.”
From The histone deacetylase HDAC11 regulates the expression of interleukin 10 and immune tolerance (2009) · cites this paper as [32]
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The histone deacetylase HDAC11 regulates the expression of interleukin 10 and immune tolerance
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- Authors
- Im, S.-H., Hueber, A., Monticelli, S., Kang, K.-H., & Rao, A.
- Affiliations
- Gwangju Institute of Science and Technology (KR)
- Harvard University (US)
- Corresponding author
- Im, Sin-Hyeog
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2004-11
- Volume
- 279
- Issue
- 45
- Pages
- 46818-46825
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2021626420
- Cited by
- 103
- References
- 24
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 2 · 2021: 2 · 2020: 1 · 2019: 5 · 2018: 3 · 2017: 1 · 2016: 6 · 2015: 3 · 2014: 3 · 2013: 5 · 2012: 8
- FWCI
- 2.60
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Cancer Institute (R01CA042471, R01 CA-42471)
- National Institute of Allergy and Infectious Diseases (R01 AI 48213, R01AI048213)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Keywords
- IL-10, IL-10 gene, chromatin structure, DNase I hypersensitive sites, T cells, Th1 cells, Th2 cells, histone modifications, gene silencing, cytokine gene expression, naive T cells, promoter repression
- MeSH terms
- Animals, Base Sequence, Binding Sites, Cell Differentiation, Chromatin, Deoxyribonuclease I, DNA, Gene Expression Regulation, Introns, Mice, Transgenic, Models, Genetic, Molecular Sequence Data, Promoter Regions, Genetic, Protein Binding, T-Lymphocytes, Transcription, Genetic, Cytokines, Interleukin-10, DNA Primers, Th1 Cells, Th2 Cells, DNA Methylation, Physical Chromosome Mapping, Gene Silencing, Chromatin Immunoprecipitation, Immunoprecipitation, Mice
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