The DOI matches its Crossref record.
The Structure of Human Cytochrome P450 2C9 Complexed with Flurbiprofen at 2.0-Å Resolution
Open accessAbstract
The structure of human P450 2C9 complexed with flurbiprofen was determined to 2.0 A by x-ray crystallography. In contrast to other structurally characterized P450 2C enzymes, 2C5, 2C8, and a 2C9 chimera, the native catalytic domain of P450 2C9 differs significantly in the conformation of the helix F to helix G region and exhibits an extra turn at the N terminus of helix A. In addition, a distinct conformation of the helix B to helix C region allows Arg-108 to hydrogen bond with Asp-293 and Asn-289 on helix I and to interact directly with the carboxylate of flurbiprofen. These interactions position the substrate for regioselective oxidation in a relatively large active site cavity and are likely to account for the high catalytic efficiency exhibited by P450 2C9 for the regioselective oxidation of several anionic non-steroidal anti-inflammatory drugs. The structure provides a basis for interpretation of a number of observations regarding the substrate selectivity of P450 2C9 and the observed effects of mutations on catalysis.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Structure and chemistry of cytochrome p450
Denisov, I. G., Makris, T. M., Sligar, S. G., & Schlichting, I. (2005) · Chemical Reviews · doi:10.1021/cr0307143 · Cited by 2,106
Mast, N., White, M. A., Bjorkhem, I., Johnson, E. F., Stout, C. D., & Pikuleva, I. A. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0803717105 · Cited by 133
Self-citationBioactivities of berberine metabolites after transformation through CYP450 isoenzymes
Li, Y., Ren, G., Wang, Y.-X., Kong, W.-J., Yang, P., Wang, Y.-M., Li, Y.-H., Yi, H., Li, Z.-R., Song, D.-Q., et al. (2011) · Journal of Translational Medicine · doi:10.1186/1479-5876-9-62 · Cited by 109
Effects of residue substitutions on the cellular abundance of proteins
Schulze, T. K., & Lindorff-Larsen, K. (2026) · eLife · doi:10.7554/elife.103721 · Cited by 3
Deguchi, T., Ab Ghani, N. S., Kurumida, Y., Iida, S., Kobayashi, K., & Saito, Y. (2025) · Briefings in Bioinformatics · doi:10.1093/bib/bbaf536 · Cited by 2
Effects of residue substitutions on the cellular abundance of proteins
Schulze, T. K., & Lindorff-Larsen, K. (2026) · eLife · doi:10.7554/elife.103721.3 · Cited by 1
Metadata
- Authors
- Wester, M. R., Yano, J. K., Schoch, G. A., Yang, C., Griffin, K. J., Stout, C. D., & Johnson, E. F.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2004-08
- Volume
- 279
- Issue
- 34
- Pages
- 35630-35637
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2154598050
- Cited by
- 440
- References
- 0
- FWCI
- 27.69
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
