The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Regulation of the Ca2+ Sensitivity of the Nonselective Cation Channel TRPM4
Open accessAbstract
TRPM4, a Ca(2+)-activated cation channel of the transient receptor potential superfamily, undergoes a fast desensitization to Ca(2+). The mechanisms underlying the alterations in Ca(2+) sensitivity are unknown. Here we show that cytoplasmic ATP reversed Ca(2+) sensitivity after desensitization, whereas mutations to putative ATP binding sites resulted in faster and more complete desensitization. Phorbol ester-induced activation of protein kinase C (PKC) increased the Ca(2+) sensitivity of wild-type TRPM4 but not of two mutants mutated at putative PKC phosphorylation sites. Overexpression of a calmodulin mutant unable to bind Ca(2+) dramatically reduced TRPM4 activation. We identified five Ca(2+)-calmodulin binding sites in TRPM4 and showed that deletion of any of the three C-terminal sites strongly impaired current activation by reducing Ca(2+) sensitivity and shifting the voltage dependence of activation to very positive potentials. Thus, the Ca(2+) sensitivity of TRPM4 is regulated by ATP, PKC-dependent phosphorylation, and calmodulin binding at the C terminus.
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Cites
Site-directed mutagenesis by overlap extension using the polymerase chain reaction
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Mechanism of calcium gating in small-conductance calcium-activated potassium channels
Xia, X.-M., Fakler, B., Rivard, A., Wayman, G., Johnson-Pais, T., Keen, J. E., Ishii, T., Hirschberg, B., Bond, C. T., Lutsenko, S., et al. (1998) · Nature · doi:10.1038/26758 · Cited by 871
TRPM4 Is a Ca2+-Activated Nonselective Cation Channel Mediating Cell Membrane Depolarization
Launay, P., Fleig, A., Perraud, A.-L., Scharenberg, A. M., Penner, R., & Kinet, J.-P. (2002) · Cell · doi:10.1016/s0092-8674(02)00719-5 · Cited by 744
Inward current channels activated by intracellular Ca in cultured cardiac cells
Colquhoun, D., Neher, E., Reuter, H., & Stevens, C. F. (1981) · Nature · doi:10.1038/294752a0 · Cited by 678
Structure of the gating domain of a Ca2+-activated K+ channel complexed with Ca2+/calmodulin
Schumacher, M. A., Rivard, A. F., Bächinger, H. P., & Adelman, J. P. (2001) · Nature · doi:10.1038/35074145 · Cited by 597
Tominaga, M., Wada, M., & Masu, M. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.111025298 · Cited by 509
Numazaki, M., Tominaga, T., Toyooka, H., & Tominaga, M. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.c200104200 · Cited by 466
Functional role of the β subunit of high conductance calcium-activated potassium channels
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TRPM5 Is a Voltage-Modulated and Ca2+-Activated Monovalent Selective Cation Channel
Hofmann, T., Chubanov, V., Gudermann, T., & Montell, C. (2003) · Current Biology · doi:10.1016/s0960-9822(03)00431-7 · Cited by 396
Structural determinant of TRPV1 desensitization interacts with calmodulin
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Cited by
Molecular pathophysiology of cerebral edema
Stokum, J. A., Gerzanich, V., & Simard, J. M. (2016) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1177/0271678x15617172 · Cited by 587
Transient Receptor Potential Channels in the Vasculature
Earley, S., & Brayden, J. E. (2015) · Physiological Reviews · doi:10.1152/physrev.00026.2014 · Cited by 420
Gating of TRP channels: a voltage connection?
Nilius, B., Talavera, K., Owsianik, G., Prenen, J., Droogmans, G., & Voets, T. (2005) · The Journal of Physiology · doi:10.1113/jphysiol.2005.088377 · Cited by 299
Self-citationDavis, M. J., Earley, S., Li, Y.-S., & Chien, S. (2023) · Physiological Reviews · doi:10.1152/physrev.00053.2021 · Cited by 273
Metadata
- Authors
- Nilius, B., Prenen, J., Tang, J., Wang, C., Owsianik, G., Janssens, A., Voets, T., & Zhu, M. X.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2005-02
- Volume
- 280
- Issue
- 8
- Pages
- 6423-6433
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1983615879
- Cited by
- 301
- References
- 32
- FWCI
- 6.31
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Nilius, B., Prenen, J., Tang, J., Wang, C., Owsianik, G., Janssens, A., Voets, T., & Zhu, M. X. (2005). Regulation of the Ca2+ Sensitivity of the Nonselective Cation Channel TRPM4. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m411089200
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