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Nox4 NAD(P)H Oxidase Mediates Hypertrophy and Fibronectin Expression in the Diabetic Kidney

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Gorin, Yves, Block, Karen, Hernandez, James, Bhandari, Basant, Wagner, Brent, Barnes, Jeffrey L., Abboud, Hanna E.·2005·Journal of Biological Chemistry
DOI 10.1074/jbc.m502412200
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Abstract

Renal hypertrophy and extracellular matrix accumulation are early features of diabetic nephropathy. We investigated the role of the NAD(P)H oxidase Nox4 in generation of reactive oxygen species (ROS), hypertrophy, and fibronectin expression in a rat model of type 1 diabetes induced by streptozotocin. Phosphorothioated antisense (AS) or sense oligonucleotides for Nox4 were administered for 2 weeks with an osmotic minipump 72 h after streptozotocin treatment. Nox4 protein expression was increased in diabetic kidney cortex compared with non-diabetic controls and was down-regulated in AS-treated animals. AS oligonucleotides inhibited NADPH-dependent ROS generation in renal cortical and glomerular homogenates. ROS generation by intact isolated glomeruli from diabetic animals was increased compared with glomeruli isolated from AS-treated animals. AS treatment reduced whole kidney and glomerular hypertrophy. Moreover, the increased expression of fibronectin protein was markedly reduced in renal cortex including glomeruli of AS-treated diabetic rats. Akt/protein kinase B and ERK1/2, two protein kinases critical for cell growth and hypertrophy, were activated in diabetes, and AS treatment almost abolished their activation. In cultured mesangial cells, high glucose increased NADPH oxidase activity and fibronectin expression, effects that were prevented in cells transfected with AS oligonucleotides. These data establish a role for Nox4 as the major source of ROS in the kidneys during early stages of diabetes and establish that Nox4-derived ROS mediate renal hypertrophy and increased fibronectin expression.

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Research works printed but not linked: 55

  1. Bilous, 1989, Diabetes, 38, 1142, 10.2337/diab.38.9.1142 · DOI
  2. Ziyadeh, 2000, Proc. Natl. Acad. Sci. U. S. A., 97, 8015, 10.1073/pnas.120055097 · DOI · Paper page
  3. Wolf, 1999, Kidney Int., 56, 393, 10.1046/j.1523-1755.1999.00590.x · DOI · Paper page
  4. Ziyadeh, 1993, Am. J. Kidney Dis., 22, 736, 10.1016/S0272-6386(12)80440-9 · DOI · Paper page
  5. Phillips, 2003, Curr. Diab. Rep., 3, 491, 10.1007/s11892-003-0013-1 · DOI
  6. Abboud, 1997, Kidney Int., 60, S3
  7. Wolf, 2003, Curr. Diab. Rep., 3, 485, 10.1007/s11892-003-0012-2 · DOI
  8. Baynes, 1991, Diabetes, 40, 405, 10.2337/diab.40.4.405 · DOI · Paper page
  9. Hinokio, 1999, Diabetologia, 42, 995, 10.1007/s001250051258 · DOI · Paper page
  10. Sano, 1998, Diabetologia, 41, 1355, 10.1007/s001250051076 · DOI · Paper page
  11. Schnackenberg, 2002, Curr. Opin. Pharmacol., 2, 121, 10.1016/S1471-4892(02)00133-9 · DOI
  12. Ha, 1994, Free Radic. Biol. Med., 16, 271, 10.1016/0891-5849(94)90152-X · DOI
  13. Onozato, 2002, Kidney Int., 61, 186, 10.1046/j.1523-1755.2002.00123.x · DOI · Paper page
  14. Koya, 2003, J. Am. Soc. Nephrol., 14, 250, 10.1097/01.ASN.0000077412.07578.44 · DOI · Paper page
  15. Lee, 2003, J. Am. Soc. Nephrol., 14, 241, 10.1097/01.ASN.0000077410.66390.0F · DOI · Paper page
  16. Kuroki, 2003, J. Am. Soc. Nephrol., 14, 216, 10.1097/01.ASN.0000077405.07888.07 · DOI
  17. Geiszt, 2004, J. Biol. Chem., 279, 51715, 10.1074/jbc.R400024200 · DOI · Paper page
  18. Cui, 1997, Proc. Natl. Acad. Sci. U. S. A., 94, 3771, 10.1073/pnas.94.8.3771 · DOI · Paper page
  19. Jones, 1995, J. Am. Soc. Nephrol., 5, 1483, 10.1681/ASN.V571483 · DOI · Paper page
  20. Lassegue, 2003, Am. J. Physiol., 285, R277, 10.1152/ajpcell.00550.2002 · DOI
  21. Li, 2003, J. Am. Soc. Nephrol., 14, 221, 10.1097/01.ASN.0000077406.67663.E7 · DOI · Paper page
  22. Kitada, 2003, Diabetes, 52, 2603, 10.2337/diabetes.52.10.2603 · DOI · Paper page
  23. Etoh, 2003, Diabetologia, 46, 1428, 10.1007/s00125-003-1205-6 · DOI · Paper page
  24. Shiose, 2001, J. Biol. Chem., 276, 1417, 10.1074/jbc.M007597200 · DOI · Paper page
  25. Geiszt, 2000, Proc. Natl. Acad. Sci. U. S. A., 97, 8010, 10.1073/pnas.130135897 · DOI · Paper page
  26. Gorin, 2003, Am. J. Physiol., 285, F219
  27. Gorin, 2004, Biochem. J., 381, 231, 10.1042/BJ20031614 · DOI
  28. Abboud, 1982, J. Clin. Investig., 69, 327, 10.1172/JCI110456 · doi:10.1172/jci110456
  29. Choudhury, 1997, Am. J. Physiol., 273, F931
  30. Chen, 2000, J. Lab. Clin. Med., 135, 309, 10.1067/mlc.2000.105616 · doi:10.1067/mlc.2000.105616
  31. Gorin, 2001, FASEB J., 15, 1909, 10.1096/fj..01-0165com · DOI
  32. Hixon, 2000, J. Clin. Investig., 106, 1011, 10.1172/JCI8252 · DOI
  33. Bueno, 2000, EMBO J., 19, 6341, 10.1093/emboj/19.23.6341 · DOI · Paper page
  34. Servant, 1996, J. Biol. Chem., 271, 16047, 10.1074/jbc.271.27.16047 · DOI · Paper page
  35. Wolf, 2004, Hypertension, 43, 693, 10.1161/01.HYP.0000120963.09029.ca · DOI
  36. Petermann, 2005, Kidney Int., 67, 157, 10.1111/j.1523-1755.2005.00066.x · DOI
  37. Fujita, 2004, Am. J. Physiol., 286, F120
  38. Isono, 2000, J. Am. Soc. Nephrol., 11, 2222, 10.1681/ASN.V11122222 · DOI
  39. Cross, 2004, Biochim. Biophys. Acta, 1657, 1, 10.1016/j.bbabio.2004.03.008 · DOI · Paper page
  40. Forbes, 2002, Diabetes, 51, 3274, 10.2337/diabetes.51.11.3274 · DOI · Paper page
  41. Hink, 2001, Circ. Res., 88, E14, 10.1161/01.RES.88.2.e14 · DOI · Paper page
  42. Kim, 2003, Diabetes Res. Clin. Pract., 60, 11, 10.1016/S0168-8227(02)00278-4 · DOI
  43. Rappaport, 1995, Kidney Int., 47, 1462, 10.1038/ki.1995.205 · DOI
  44. Green, 2004, Diabetes, 53, 110, 10.2337/diabetes.53.2007.S110 · DOI · Paper page
  45. DeRubertis, 2004, Diabetes, 53, 762, 10.2337/diabetes.53.3.762 · DOI
  46. Yamagishi, 2001, Diabetes, 50, 1491, 10.2337/diabetes.50.6.1491 · DOI · Paper page
  47. Lin, 2005, J. Biol. Chem., 280, 4617, 10.1074/jbc.M411863200 · DOI · Paper page
  48. Sheu, 2004, Mol. Pharmacol., 66, 187, 10.1124/mol.66.1.187 · DOI
  49. Hua, 2003, J. Biol. Chem., 278, 33951, 10.1074/jbc.M302823200 · DOI
  50. Chen, 2004, J. Biol. Chem., 279, 35079, 10.1074/jbc.M404859200 · DOI
  51. Kimura, 2005, Hypertension, 45, 860, 10.1161/01.HYP.0000163462.98381.7f · DOI · Paper page
  52. Carey, 2003, Trends Endocrinol. Metab., 14, 274, 10.1016/S1043-2760(03)00111-5 · DOI · Paper page
  53. Haneda, 2003, J. Am. Soc. Nephrol., 14, 1374, 10.1097/01.ASN.0000064500.89551.76 · DOI
  54. Ghosh Choudhury, 2004, Cell. Signal., 16, 31, 10.1016/S0898-6568(03)00094-9 · DOI
  55. Crean, 2002, J. Biol. Chem., 277, 44187, 10.1074/jbc.M203715200 · DOI

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Metadata

Authors
Gorin, Y., Block, K., Hernandez, J., Bhandari, B., Wagner, B., Barnes, J. L., & Abboud, H. E.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2005-11
Volume
280
Issue
47
Pages
39616-39626
ISSN
0021-9258 (ISSN-L)
DOI
10.1074/jbc.m502412200
OpenAlex
W2012258975
Cited by
497
References
7
FWCI
13.45
Type
journal article
Language
en
Open access
hybrid
Licence

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CoChat. (2026). Citation check: Nox4 NAD(P)H Oxidase Mediates Hypertrophy and Fibronectin Expression in the Diabetic Kidney. https://app.cochat.ai/papers/10.1074/jbc.m502412200
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Gorin, Y., Block, K., Hernandez, J., Bhandari, B., Wagner, B., Barnes, J. L., & Abboud, H. E. (2005). Nox4 NAD(P)H Oxidase Mediates Hypertrophy and Fibronectin Expression in the Diabetic Kidney. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m502412200

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