The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
APOBEC3B and APOBEC3F Inhibit L1 Retrotransposition by a DNA Deamination-independent Mechanism
Open accessAbstract
The most common transposable genetic element in humans, long interspersed element 1 (L1), constitutes about 20% of the genome. The activity of L1 and related transposons such as Alu elements causes disease and contributes to speciation. Little is known about the cellular mechanisms that control their spread. We show that expression of human APOBEC3B or APOBEC3F decreased the rate of L1 retrotransposition by 5-10-fold. Expression of two related proteins, APOBEC3D or APOBEC3G, had little effect. The mechanism of L1 inhibition did not correlate with an obvious subcellular protein distribution as APOBEC3B appeared predominantly nuclear and APOBEC3F was mostly cytosolic. Two lines of evidence indicated that these APOBEC3 proteins use a deamination-independent mechanism to inhibit L1. First, a catalytically inactive APOBEC3B mutant maintained L1 inhibition activity. Second, cDNA strand-specific C --> T hypermutations were not detected among L1 elements that had replicated in the presence of APOBEC3B or APOBEC3F. In addition, lower levels of retrotransposed L1 DNA accumulated in the presence of APOBEC3B and APOBEC3F. Together, these data combined to suggest a model in which APOBEC3B or APOBEC3F provide a preintegration barrier to L1 retrotransposition. A particularly high level of APOBEC3F protein in human testes and an inverse correlation between L1 activity and APOBEC3 gene number suggest the relevance of this mechanism to mammals.
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Cites
Initial sequencing and analysis of the human genome
International Human Genome Sequencing Consortium, Whitehead Institute for Biomedical Research, C. F. G. R., Lander, E. S., Linton, L. M., Birren, B., Nusbaum, C., Zody, M. C., Baldwin, J., Devon, K., Dewar, K., et al. (2001) · Nature · doi:10.1038/35057062 · Cited by 24,216
Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein
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A Human Protein Atlas for Normal and Cancer Tissues Based on Antibody Proteomics
Uhlén, M., Björling, E., Agaton, C., Szigyarto, C. A.-K., Amini, B., Andersen, E., Andersson, A.-C., Angelidou, P., Asplund, A., Asplund, C., et al. (2005) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m500279-mcp200 · Cited by 1,540
Broad antiretroviral defence by human APOBEC3G through lethal editing of nascent reverse transcripts
Mangeat, B., Turelli, P., Caron, G., Friedli, M., Perrin, L., & Trono, D. (2003) · Nature · doi:10.1038/nature01709 · Cited by 1,486
DNA Deamination Mediates Innate Immunity to Retroviral Infection
Harris, R. S., Bishop, K. N., Sheehy, A. M., Craig, H. M., Petersen-Mahrt, S. K., Watt, I. N., Neuberger, M. S., & Malim, M. H. (2003) · Cell · doi:10.1016/s0092-8674(03)00423-9 · Cited by 1,266
Self-citationHot L1s account for the bulk of retrotransposition in the human population
Brouha, B., Schustak, J., Badge, R. M., Lutz-Prigge, S., Farley, A. H., Moran, J. V., & Kazazian, H. H. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0831042100 · Cited by 1,073
The cytidine deaminase CEM15 induces hypermutation in newly synthesized HIV-1 DNA
Zhang, H., Yang, B., Pomerantz, R. J., Zhang, C., Arunachalam, S. C., & Gao, L. (2003) · Nature · doi:10.1038/nature01707 · Cited by 1,059
High frequency retrotransposition in cultured mammalian cells
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HIV-1 Vif protein binds the editing enzyme APOBEC3G and induces its degradation
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Biology of Mammalian L1 Retrotransposons
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Cited by
Harris, R. S., & Dudley, J. P. (2015) · Virology · doi:10.1016/j.virol.2015.03.012 · Cited by 551
Self-citationRestricting retrotransposons: a review
Goodier, J. L. (2016) · Mobile DNA · doi:10.1186/s13100-016-0070-z · Cited by 436
The APOBEC Protein Family: United by Structure, Divergent in Function
Salter, J. D., Bennett, R. P., & Smith, H. C. (2016) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2016.05.001 · Cited by 408
Simmonds, P. (2020) · mSphere · doi:10.1128/msphere.00408-20 · Cited by 245
Somatic expression of LINE-1 elements in human tissues
Belancio, V. P., Roy-Engel, A. M., Pochampally, R. R., & Deininger, P. (2010) · Nucleic Acids Research · doi:10.1093/nar/gkq132 · Cited by 238
Metadata
- Authors
- Stenglein, M. D., & Harris, R. S.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2006-06
- Volume
- 281
- Issue
- 25
- Pages
- 16837-16841
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1966477735
- Cited by
- 277
- References
- 36
- FWCI
- 35.24
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Stenglein, M. D., & Harris, R. S. (2006). APOBEC3B and APOBEC3F Inhibit L1 Retrotransposition by a DNA Deamination-independent Mechanism. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m602367200
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