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The hemolymph of the ascidian Styela plicata (Chordata‐Tunicata) contains heparin inside basophil‐like cells and a unique sulfated galactoglucan in the plasma

de Barros, Cintia M., Andrade, Leonardo R., Allodi, Silvana, Viskov, Christian, Mourier, Pierre A., Cavalcante, Moisés C.M., Straus, Anita H., Takahashi, Helio K., Pomin, Vitor H., Carvalho, Vinicius F., Martins, Marco A., Pavão, Mauro S.G.·2007·Journal of Biological Chemistry
DOI 10.1074/jbc.m604056200
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Abstract

The hemolymph of ascidians (Chordata-Tunicata) contains different types of hemocytes embedded in a liquid plasma. In the present study, heparin and a sulfated heteropolysaccharide were purified from the hemolymph of the ascidian Styela plicata. The heteropolysaccharide occurs free in the plasma, is composed of glucose ( approximately 60%) and galactose ( approximately 40%), and is highly sulfated. Heparin, on the other hand, occurs in the hemocytes, and high performance liquid chromatography of the products formed by degradation with specific lyases revealed that it is composed mainly by the disaccharides DeltaUA(2SO(4))-1-->4-beta-d-GlcN(SO(4)) (39.7%) and DeltaUA(2SO(4))-1-->4-beta-d-GlcN(SO(4))(6SO(4)) (38.2%). Small amounts of the 3-O-sulfated disaccharides DeltaUA(2SO(4))-1-->4-beta-d-GlcN(SO(4))(3SO(4)) (9.8%) and DeltaUA(2SO(4))-1-->4-beta-d-GlcN(SO(4))(3SO(4))(6SO(4)) (3.8%) were also detected. These 3-O-sulfated disaccharides were demonstrated to be essential for the binding of the hemocyte heparin to antithrombin III. Electron microscopy techniques were used to characterize the ultrastructure of the hemocytes and to localize heparin and histamine in these cells. At least five cell types were recognized and classified as univacuolated and multivacuolated cells, amebocytes, hemoblasts, and granulocytes. Immunocytochemistry showed that heparin and histamine co-localize in intracellular granules of only one type of hemocyte, the granulocyte. These results show for the f…

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Research works printed but not linked: 77

  1. Casu, 2001, Adv. Carbohydr. Chem. Biochem., 57, 159, 10.1016/S0065-2318(01)57017-1 · DOI
  2. Powell, 2004, Glycobiology, 14, 17R, 10.1093/glycob/cwh051 · DOI
  3. Yurt, 1977, J. Biol. Chem., 252, 518, 10.1016/S0021-9258(17)32747-3 · DOI
  4. Robinson, 1978, J. Biol. Chem., 253, 6687, 10.1016/S0021-9258(17)37973-5 · DOI
  5. Metcalfe, 1979, J. Clin. Invest., 64, 1537, 10.1172/JCI109613 · DOI
  6. Metcalfe, 1980, J. Biol. Chem., 255, 11753, 10.1016/S0021-9258(19)70198-7 · DOI
  7. Bland, 1982, J. Biol. Chem., 257, 8661, 10.1016/S0021-9258(18)34179-6 · DOI
  8. Yurt, 1977, J. Immunol., 118, 1201, 10.4049/jimmunol.118.4.1201 · DOI
  9. Schwartz, 1981, J. Immunol., 126, 2071, 10.4049/jimmunol.126.6.2071 · DOI
  10. MacDermott, 1985, J. Exp. Med., 162, 1771, 10.1084/jem.162.6.1771 · DOI
  11. Razin, 1982, J. Biol. Chem., 257, 7229, 10.1016/S0021-9258(18)34561-7 · DOI
  12. Nakano, 1985, J. Exp. Med., 162, 1025, 10.1084/jem.162.3.1025 · DOI · Paper page
  13. Wedemeyer, 2000, Curr. Opin. Immunol., 12, 624, 10.1016/S0952-7915(00)00154-0 · DOI
  14. Dietrich, 1985, Biochim. Biophys. Acta., 843, 1, 10.1016/0304-4165(85)90041-8 · DOI
  15. Pejler, 1987, J. Biol. Chem., 262, 11413, 10.1016/S0021-9258(18)60822-1 · DOI
  16. Dietrich, 1989, Int. J. Biol. Macromol., 11, 361, 10.1016/0141-8130(89)90008-1 · DOI
  17. Nader, 1999, Braz. J. Med. Biol. Res., 32, 529, 10.1590/S0100-879X1999000500005 · DOI
  18. Arumugam, 2004, Indian J. Exp. Biol., 42, 529
  19. Cesaretti, 2004, Glycobiology, 14, 1275, 10.1093/glycob/cwh128 · DOI
  20. Luppi, 2005, Biomacromolecules, 6, 1672, 10.1021/bm049196b · DOI
  21. Hovingh, 1982, J. Biol. Chem., 257, 9840, 10.1016/S0021-9258(18)34147-4 · DOI
  22. Dietrich, 1999, Biochim. Biophys. Acta, 1428, 273, 10.1016/S0304-4165(99)00087-2 · DOI
  23. Chavante, 2000, Int. J. Biol. Macromol., 27, 49, 10.1016/S0141-8130(99)00114-2 · DOI
  24. Tirumalai, 2001, Mol. Reprod. Dev., 58, 54, 10.1002/1098-2795(200101)58:1<54::AID-MRD8>3.0.CO;2-I · DOI
  25. Demir, 2001, Clin. Appl. Thromb. Hemost., 7, 44, 10.1177/107602960100700110 · DOI
  26. Cavalcante, 2000, J. Biol. Chem., 275, 36189, 10.1074/jbc.M005830200 · DOI
  27. Cavalcante, 2002, J. Struct. Biol., 137, 313, 10.1016/S1047-8477(02)00007-2 · DOI · Paper page
  28. Pavão, 2002, An. Acad. Bras. Cienc., 74, 105, 10.1590/S0001-37652002000100007 · DOI
  29. Sawada, 1993, Biol. Bull., 184, 87, 10.2307/1542382 · DOI
  30. Radford, 1998, Acta Zool., 79, 44
  31. Menzel, 2002, Dev. Comp. Immunol., 26, 505, 10.1016/S0145-305X(02)00010-1 · DOI
  32. Hirose, 2003, Zoolog. Sci., 20, 647, 10.2108/zsj.20.647 · DOI
  33. Hirose, 2003, Zoolog. Sci., 20, 387, 10.2108/zsj.20.387 · DOI
  34. Cardoso, 1994, Braz. J. Med. Biol. Res., 27, 509
  35. Brown, 1959, J. Biol. Chem., 234, 2948, 10.1016/S0021-9258(18)69701-7 · DOI
  36. Peddie, 1994, Ann. N. Y. Acad. Sci., 712, 332, 10.1111/j.1749-6632.1994.tb33587.x · DOI
  37. Farndale, 1986, Biochim. Biophys. Acta, 883, 173, 10.1016/0304-4165(86)90306-5 · DOI
  38. Dietrich, 1976, Anal. Biochem., 70, 645, 10.1016/0003-2697(76)90496-6 · DOI
  39. Mourier, 2004, Anal. Biochem., 332, 299, 10.1016/j.ab.2004.06.020 · DOI
  40. Bitter, 1962, Anal. Biochem., 4, 330, 10.1016/0003-2697(62)90095-7 · DOI
  41. Shively, 1976, Biochemistry, 15, 3932, 10.1021/bi00663a005 · DOI
  42. Dubois, 1956, Anal. Chem., 28, 350, 10.1021/ac60111a017 · DOI · Paper page
  43. Saito, 1968, J. Biol. Chem., 243, 1536, 10.1016/S0021-9258(18)93575-1 · DOI
  44. Straus, 1992, Anal. Biochem., 201, 1, 10.1016/0003-2697(92)90167-6 · DOI
  45. Fleury, 1951, Ann. Biol. Clin., 9, 453
  46. Snyder, 1966, J. Pharmacol. Exp. Ther., 153, 544
  47. Correa, 1981, Brain Res., 205, 445, 10.1016/0006-8993(81)90359-0 · DOI
  48. Fuke, 1993, Acta Zool., 74, 61, 10.1111/j.1463-6395.1993.tb01221.x · DOI
  49. Shishikura, 1996, Comp. Biochem. Physiol. B Biochem. Mol. Biol., 114, 1, 10.1016/0305-0491(95)02110-8 · DOI
  50. Shishikura, 1997, Comp. Biochem. Physiol. B Biochem. Mol. Biol., 118, 131, 10.1016/S0305-0491(97)00217-4 · DOI
  51. Azumi, 1996, Zool. Sci., 13, 365, 10.2108/zsj.13.365 · DOI
  52. Akita, 1995, Cell Struct. Funct., 20, 81, 10.1247/csf.20.81 · DOI
  53. Kumazaki, 1990, J. Biochem. (Tokyo), 107, 409, 10.1093/oxfordjournals.jbchem.a123058 · DOI
  54. Yokosawa, 1985, J. Biochem. (Tokyo), 97, 1621, 10.1093/oxfordjournals.jbchem.a135219 · DOI
  55. Takahashi, 1995, Eur. J. Biochem., 233, 778, 10.1111/j.1432-1033.1995.778_3.x · DOI
  56. Green, 2003, Dev. Comp. Immunol., 27, 3, 10.1016/S0145-305X(02)00067-8 · DOI
  57. Pearce, 2001, Dev. Comp. Immunol., 25, 377, 10.1016/S0145-305X(01)00011-8 · DOI
  58. Kenjo, 2001, J. Bio.l Chem., 276, 19959, 10.1074/jbc.M011723200 · DOI
  59. Pavão, 1989, Carbohydr. Res., 189, 374, 10.1016/0008-6215(89)84115-1 · DOI
  60. Pavão, 1989, J. Biol. Chem., 264, 9972, 10.1016/S0021-9258(18)81755-0 · DOI
  61. Pavão, 1990, Carbohydr. Res., 208, 153, 10.1016/0008-6215(90)80095-K · DOI
  62. Pavão, 1994, Biochim. Biophys. Acta, 1199, 229, 10.1016/0304-4165(94)90001-9 · DOI
  63. Mourão, 1995, J. Biol. Chem., 270, 3132, 10.1074/jbc.270.7.3132 · DOI
  64. Pavão, 1994, J. Exp. Zool., 269, 89, 10.1002/jez.1402690202 · DOI
  65. Pavão, 1996, Braz. J. Med. Biol. Res., 29, 1227
  66. Pavão, 1998, J. Biol. Chem., 273, 27848, 10.1074/jbc.273.43.27848 · DOI
  67. Gandra, 2000, Glycobiology, 10, 1333, 10.1093/glycob/10.12.1333 · DOI
  68. Pavão, 1995, J. Biol. Chem., 270, 31027, 10.1074/jbc.270.52.31027 · DOI
  69. Mourão, 1997, Carbohydr. Res., 300, 315, 10.1016/S0008-6215(97)00061-X · DOI
  70. Lindahl, 1980, Proc. Natl. Acad. Sci. U. S. A., 77, 6551, 10.1073/pnas.77.11.6551 · DOI · Paper page
  71. Petitou, 2003, Biochimie (Paris), 85, 83, 10.1016/S0300-9084(03)00078-6 · DOI
  72. Lindahl, 1994, Thromb. Res., 75, 1, 10.1016/0049-3848(94)90136-8 · DOI
  73. Martinez-Silvestre, 2004, Vet. Rec., 155, 266, 10.1136/vr.155.9.266 · DOI
  74. Martinez-Silvestre, 2005, Res. Vet. Sci., 78, 127, 10.1016/j.rvsc.2004.07.009 · DOI
  75. Zucker-Franklin, 1964, J. Exp. Med., 120, 569, 10.1084/jem.120.4.569 · DOI
  76. Horner, 1971, J. Biol. Chem., 246, 231, 10.1016/S0021-9258(18)62554-2 · DOI
  77. Kobayashi, 1972, Anal. Biochem., 46, 85, 10.1016/0003-2697(72)90397-1 · DOI

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Metadata

Authors
de Barros, C. M., Andrade, L. R., Allodi, S., Viskov, C., Mourier, P. A., Cavalcante, M. C. M., Straus, A. H., Takahashi, H. K., Pomin, V. H., Carvalho, V. F., Martins, M. A., & Pavão, M. S. G.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2007-01
Volume
282
Issue
3
Pages
1615-1626
ISSN
0021-9258
DOI
10.1074/jbc.m604056200
Cited by
48
References
0
Language
en
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CoChat. (2026). Citation check: The hemolymph of the ascidian Styela plicata (Chordata‐Tunicata) contains heparin inside basophil‐like cells and a unique sulfated galactoglucan in the plasma. https://app.cochat.ai/papers/10.1074/jbc.m604056200
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de Barros, C. M., Andrade, L. R., Allodi, S., Viskov, C., Mourier, P. A., Cavalcante, M. C. M., Straus, A. H., Takahashi, H. K., Pomin, V. H., Carvalho, V. F., Martins, M. A., & Pavão, M. S. G. (2007). The hemolymph of the ascidian Styela plicata (Chordata‐Tunicata) contains heparin inside basophil‐like cells and a unique sulfated galactoglucan in the plasma. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m604056200