The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
Replication Protein A Directs Loading of the DNA Damage Checkpoint Clamp to 5′-DNA Junctions
Open accessAbstract
The heterotrimeric checkpoint clamp comprises the Saccharomyces cerevisiae Rad17, Mec3, and Ddc1 subunits (Rad17/3/1, the 9-1-1 complex in humans). This DNA damage response factor is loaded onto DNA by the Rad24-RFC (replication factor C-like complex with Rad24) clamp loader and ATP. Although Rad24-RFC alone does not bind to naked partial double-stranded DNA, coating of the single strand with single-stranded DNA-binding protein RPA (replication protein A) causes binding of Rad24-RFC via interactions with RPA. However, RPA-mediated binding is abrogated when the DNA is coated with RPA containing a rpa1-K45E (rfa1-t11) mutation. These properties allowed us to determine the role of RPA in clamp-loading specificity. The Rad17/3/1 clamp is loaded with comparable efficiency onto naked primer/template DNA with either a 3'-junction or a 5'-junction. Remarkably, when the DNA was coated with RPA, loading of Rad17/3/1 at 3'-junctions was completely inhibited, thereby providing specificity to loading at 5'-junctions. However, Rad17/3/1 loaded at 5'-junctions can slide across double-stranded DNA to nearby 3'-junctions and thereby affect the activity of proteins that act at 3'-termini. These studies show a unique specificity of the checkpoint loader for 5'-junctions of RPA-coated DNA. The implications of this specificity for checkpoint function are discussed.
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“The PCNA-like clamp 9-1-1 complex (Rad9-Rad1-Hus1) is recruited preferentially to the 5′-primed ssDNA/dsDNA junction by RPA and the clamp loader Rad17-RFC complex [102–105].”
From Functional interplay between ATM/ATR-mediated DNA damage response and DNA repair pathways in oxidative stress (2014) · cites this paper as [105]
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- Fortune, 2006, Nucleic Acids Res
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ATR: an essential regulator of genome integrity
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“Significantly, full activation of the ATR-dependent replication checkpoint requires downstream repriming and recruitment of the 9-1-1 checkpoint clamp to the 50 phosphorylated end of the resulting RPAcoated gap (Zou and Elledge 2003; Majka et al. 2006).”
BackgroundThe Rad9-Hus1-Rad1 Checkpoint Clamp Regulates Interaction of TopBP1 with ATR
Lee, J., Kumagai, A., & Dunphy, W. G. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m704635200 · Cited by 294
Metadata
- Authors
- Majka, J., Binz, S. K., Wold, M. S., & Burgers, P. M. J.
- Affiliations
- Washington University in St. Louis (US)
- University of Iowa (US)
- Corresponding author
- Burgers, Peter M.J.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2006-09
- Volume
- 281
- Issue
- 38
- Pages
- 27855-27861
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2161481116
- Cited by
- 205
- References
- 43
- Citations by year
- 2026: 8 · 2025: 6 · 2024: 10 · 2023: 3 · 2022: 10 · 2021: 10 · 2020: 6 · 2019: 5 · 2018: 7 · 2017: 9 · 2016: 8 · 2015: 12 · 2014: 16 · 2013: 13 · 2012: 9
- FWCI
- 5.97
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (GM44721, GM-32431, R01GM044721, R01GM032431)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- replication protein A, clamp loading, DNA damage response, single-stranded DNA binding protein, Saccharomyces cerevisiae
- MeSH terms
- Adenosine Triphosphatases, DNA Damage, DNA Helicases, DNA-Binding Proteins, Endonucleases, Nuclear Proteins, Substrate Specificity, Cell Cycle Proteins, Proliferating Cell Nuclear Antigen, Saccharomyces cerevisiae Proteins, DNA Repair Enzymes, Intracellular Signaling Peptides and Proteins, Replication Protein A, Replication Protein C
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