The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Biochemical and Functional Characterization of Orai Proteins
Open accessAbstract
Stimulation of immune cells triggers Ca2+ entry through store-operated Ca2+ release-activated Ca2+ channels, promoting nuclear translocation of the transcription factor NFAT. Through genome-wide RNA interference screens in Drosophila, we and others identified olf186-F (Drosophila Orai, dOrai) and dStim as critical components of store-operated Ca2+ entry and showed that dOrai and its human homologue Orai1 are pore subunits of the Ca2+ release-activated Ca2+ channel. Here we report that Orai1 is predominantly responsible for store-operated Ca2+ influx in human embryonic kidney 293 cells and human T cells and fibroblasts, although its paralogue Orai3 can partly compensate in the absence of functional Orai1. All three mammalian Orai are widely expressed at the mRNA level, and all three are incorporated into the plasma membrane. In human embryonic kidney 293 cells, Orai1 is glycosylated at an asparagine residue in the predicted second extracellular loop, but mutation of the residue does not compromise function. STIM1 and Orai1 colocalize after store depletion, but Orai1 does not associate detectably with STIM1 in glycerol gradient centrifugation or coimmunoprecipitation experiments. Glutamine substitutions in two conserved glutamate residues, located within predicted transmembrane helices of Drosophila Orai and human Orai1, greatly diminish store-operated Ca2+ influx, and primary T cells ectopically expressing mutant E106Q and E190Q Orai1 proteins show reduced proliferation and cytokine secretion. Together, these data establish Orai1 as a predominant mediator of store-operated calcium entry, proliferation, and cytokine production in T cells.
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Cites
A model for receptor-regulated calcium entry
Putney, J. W. (1986) · Cell Calcium · doi:10.1016/0143-4160(86)90026-6 · Cited by 2,575
A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function
Feske, S., Gwack, Y., Prakriya, M., Srikanth, S., Puppel, S.-H., Tanasa, B., Hogan, P. G., Lewis, R. S., Daly, M., & Rao, A. (2006) · Nature · doi:10.1038/nature04702 · Cited by 2,288
Self-citationSTIM Is a Ca2+ Sensor Essential for Ca2+-Store-Depletion-Triggered Ca2+ Influx
Liou, J., Kim, M. L., Do Heo, W., Jones, J. T., Myers, J. W., Ferrell, J. E., & Meyer, T. (2005) · Current Biology · doi:10.1016/j.cub.2005.05.055 · Cited by 2,136
Transcriptional regulation by calcium, calcineurin, and NFAT
Hogan, P. G., Chen, L., Nardone, J., & Rao, A. (2003) · Genes & Development · doi:10.1101/gad.1102703 · Cited by 1,991
Self-citationSTIM1, an essential and conserved component of store-operated Ca2 + channel function
Roos, J., DiGregorio, P. J., Yeromin, A. V., Ohlsen, K., Lioudyno, M., Zhang, S., Safrina, O., Kozak, J. A., Wagner, S. L., Cahalan, M. D., et al. (2005) · The Journal of Cell Biology · doi:10.1083/jcb.200502019 · Cited by 1,828
Capacitative calcium entry revisited
Putney, J. W. (1990) · Cell Calcium · doi:10.1016/0143-4160(90)90016-n · Cited by 1,487
Zhang, S. L., Yu, Y., Roos, J., Kozak, J. A., Deerinck, T. J., Ellisman, M. H., Stauderman, K. A., & Cahalan, M. D. (2005) · Nature · doi:10.1038/nature04147 · Cited by 1,389
Orai1 is an essential pore subunit of the CRAC channel
Prakriya, M., Feske, S., Gwack, Y., Srikanth, S., Rao, A., & Hogan, P. G. (2006) · Nature · doi:10.1038/nature05122 · Cited by 1,343
Self-citationCRACM1 Is a Plasma Membrane Protein Essential for Store-Operated Ca 2+ Entry
Vig, M., Peinelt, C., Beck, A., Koomoa, D. L., Rabah, D., Koblan-Huberson, M., Kraft, S., Turner, H., Fleig, A., Penner, R., et al. (2006) · Science · doi:10.1126/science.1127883 · Cited by 1,321
Zhang, S. L., Yeromin, A. V., Zhang, X. H.-F., Yu, Y., Safrina, O., Penna, A., Roos, J., Stauderman, K. A., & Cahalan, M. D. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0603161103 · Cited by 866
Cited by
Store-Operated Calcium Channels
Prakriya, M., & Lewis, R. S. (2015) · Physiological Reviews · doi:10.1152/physrev.00020.2014 · Cited by 1,239
Calcium signalling in lymphocyte activation and disease
Feske, S. (2007) · Nature Reviews Immunology · doi:10.1038/nri2152 · Cited by 1,043
Self-citationMolecular Basis of Calcium Signaling in Lymphocytes: STIM and ORAI
Hogan, P. G., Lewis, R. S., & Rao, A. (2010) · Annual Review of Immunology · doi:10.1146/annurev.immunol.021908.132550 · Cited by 760
Self-citationThe functional network of ion channels in T lymphocytes
Cahalan, M. D., & Chandy, K. G. (2009) · Immunological Reviews · doi:10.1111/j.1600-065x.2009.00816.x · Cited by 599
Oh-hora, M., Yamashita, M., Hogan, P. G., Sharma, S., Lamperti, E., Chung, W., Prakriya, M., Feske, S., & Rao, A. (2008) · Nature Immunology · doi:10.1038/ni1574 · Cited by 593
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Metadata
- Authors
- Gwack, Y., Srikanth, S., Feske, S., Cruz-Guilloty, F., Oh-hora, M., Neems, D. S., Hogan, P. G., & Rao, A.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2007-06
- Volume
- 282
- Issue
- 22
- Pages
- 16232-16243
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2059989081
- Cited by
- 381
- References
- 33
- FWCI
- 16.81
- Type
- journal article
- Language
- en
- Open access
- hybrid
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