The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Genome-wide RNAi screen of Ca 2+ influx identifies genes that regulate Ca 2+ release-activated Ca 2+ channel activity
Open accessAbstract
Recent studies by our group and others demonstrated a required and conserved role of Stim in store-operated Ca 2+ influx and Ca 2+ release-activated Ca 2+ (CRAC) channel activity. By using an unbiased genome-wide RNA interference screen in Drosophila S2 cells, we now identify 75 hits that strongly inhibited Ca 2+ influx upon store emptying by thapsigargin. Among these hits are 11 predicted transmembrane proteins, including Stim , and one, olf186-F , that upon RNA interference-mediated knockdown exhibited a profound reduction of thapsigargin-evoked Ca 2+ entry and CRAC current, and upon overexpression a 3-fold augmentation of CRAC current. CRAC currents were further increased to 8-fold higher than control and developed more rapidly when olf186-F was cotransfected with Stim . olf186-F is a member of a highly conserved family of four-transmembrane spanning proteins with homologs from Caenorhabditis elegans to human. The endoplasmic reticulum (ER) Ca 2+ pump sarco-/ER calcium ATPase (SERCA) and the single transmembrane-soluble N -ethylmaleimide-sensitive (NSF) attachment receptor (SNARE) protein Syntaxin5 also were required for CRAC channel activity, consistent with a signaling pathway in which Stim senses Ca 2+ depletion within the ER, translocates to the plasma membrane, and interacts with olf186-F to trigger CRAC channel activity.
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Cites
Store-Operated Calcium Channels
Parekh, A. B., & Putney, J. W. (2005) · Physiological Reviews · doi:10.1152/physrev.00057.2003 · Cited by 2,089
Functional genomics reveals genes involved in protein secretion and Golgi organization
Bard, F., Casano, L., Mallabiabarrena, A., Wallace, E., Saito, K., Kitayama, H., Guizzunti, G., Hu, Y., Wendler, F., DasGupta, R., et al. (2006) · Nature · doi:10.1038/nature04377 · Cited by 372
Cited by
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Smith-Garvin, J. E., Koretzky, G. A., & Jordan, M. S. (2009) · Annual Review of Immunology · doi:10.1146/annurev.immunol.021908.132706 · Cited by 2,144
Store-Operated Calcium Channels
Parekh, A. B., & Putney, J. W. (2005) · Physiological Reviews · doi:10.1152/physrev.00057.2003 · Cited by 2,089
Phosphoinositides: Tiny Lipids With Giant Impact on Cell Regulation
Balla, T. (2013) · Physiological Reviews · doi:10.1152/physrev.00028.2012 · Cited by 1,638
The endoplasmic reticulum: structure, function and response to cellular signaling
Schwarz, D. S., & Blower, M. D. (2016) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-015-2052-6 · Cited by 1,613
Metadata
- Authors
- Zhang, S. L., Yeromin, A. V., Zhang, X. H.-F., Yu, Y., Safrina, O., Penna, A., Roos, J., Stauderman, K. A., & Cahalan, M. D.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2006-06-13
- Volume
- 103
- Issue
- 24
- Pages
- 9357-9362
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2118988881
- Cited by
- 866
- References
- 2
- FWCI
- 27.47
- Type
- journal article
- Language
- en
- Open access
- green
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- Zhang, S. L., Yeromin, A. V., Zhang, X. H.-F., Yu, Y., Safrina, O., Penna, A., Roos, J., Stauderman, K. A., & Cahalan, M. D. (2006). Genome-wide RNAi screen of Ca 2+ influx identifies genes that regulate Ca 2+ release-activated Ca 2+ channel activity. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0603161103
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