The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
Carbonic Anhydrase in the Scleractinian Coral Stylophora pistillata
Open accessAbstract
Carbonic anhydrases (CA) play an important role in biomineralization from invertebrates to vertebrates. Previous experiments have investigated the role of CA in coral calcification, mainly by pharmacological approaches. This study reports the molecular cloning, sequencing, and immunolocalization of a CA isolated from the scleractinian coral Stylophora pistillata, named STPCA. Results show that STPCA is a secreted form of alpha-CA, which possesses a CA catalytic function, similar to the secreted human CAVI. We localized this enzyme at the calicoblastic ectoderm level, which is responsible for the precipitation of the skeleton. This localization supports the role of STPCA in the calcification process. In symbiotic scleractinian corals, calcification is stimulated by light, a phenomenon called "light-enhanced calcification" (LEC). The mechanism by which symbiont photosynthesis stimulates calcification is still enigmatic. We tested the hypothesis that coral genes are differentially expressed under light and dark conditions. By real-time PCR, we investigated the differential expression of STPCA to determine its role in the LEC phenomenon. Results show that the STPCA gene is expressed 2-fold more during the dark than the light. We suggest that in the dark, up-regulation of the STPCA gene represents a mechanism to cope with night acidosis.
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Cites
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1016/0003-2697(87)90021-2 · Cited by 46,426
MRBAYES: Bayesian inference of phylogenetic trees.
Huelsenbeck, J. P., & Ronquist, F. (2001) · Bioinformatics · doi:10.1093/bioinformatics/17.8.754 · Cited by 21,937
A Simple, Fast, and Accurate Algorithm to Estimate Large Phylogenies by Maximum Likelihood
Guindon, S., & Gascuel, O. (2003) · Systematic Biology · doi:10.1080/10635150390235520 · Cited by 16,806
Carbonic anhydrases: novel therapeutic applications for inhibitors and activators
Supuran, C. T. (2008) · Nature Reviews Drug Discovery · doi:10.1038/nrd2467 · Cited by 3,192
Self-citationSequence and structure-based prediction of eukaryotic protein phosphorylation sites
Blom, N., Gammeltoft, S., & Brunak, S. (1999) · Journal of Molecular Biology · doi:10.1006/jmbi.1999.3310 · Cited by 3,188
Locating proteins in the cell using TargetP, SignalP and related tools
Emanuelsson, O., Brunak, S., von Heijne, G., & Nielsen, H. (2007) · Nature Protocols · doi:10.1038/nprot.2007.131 · Cited by 3,188
ProtTest: selection of best-fit models of protein evolution
Abascal, F., Zardoya, R., & Posada, D. (2005) · Bioinformatics · doi:10.1093/bioinformatics/bti263 · Cited by 3,063
Tmbase-A database of membrane spanning protein segments
Hofmann, K., & Stoffel, W. (1993) · Medical Entomology and Zoology · Cited by 2,240
The Carbon Dioxide Hydration Activity of Carbonic Anhydrase
Khalifah, R. G. (1971) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)62326-9 · Cited by 1,913
Cited by
Supuran, C. T. (2010) · Bioorganic & Medicinal Chemistry Letters · doi:10.1016/j.bmcl.2010.05.009 · Cited by 616
Self-citationCarbonic anhydrases in anthozoan corals—A review
Bertucci, A., Moya, A., Tambutté, S., Allemand, D., Supuran, C. T., & Zoccola, D. (2013) · Bioorganic & Medicinal Chemistry · doi:10.1016/j.bmc.2012.10.024 · Cited by 243
Self-citationSaenger, C., Affek, H. P., Felis, T., Thiagarajan, N., Lough, J. M., & Holcomb, M. (2012) · Geochimica et Cosmochimica Acta · doi:10.1016/j.gca.2012.09.035 · Cited by 152
Le Roy, N., Jackson, D. J., Marie, B., Ramos-Silva, P., & Marin, F. (2014) · Frontiers in Zoology · doi:10.1186/s12983-014-0075-8 · Cited by 131
Uchikawa, J., & Zeebe, R. E. (2012) · Geochimica et Cosmochimica Acta · doi:10.1016/j.gca.2012.07.022 · Cited by 111
Metadata
- Authors
- Moya, A., Tambutté, S., Bertucci, A., Tambutté, E., Lotto, S., Vullo, D., Supuran, C. T., Allemand, D., & Zoccola, D.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2008-09
- Volume
- 283
- Issue
- 37
- Pages
- 25475-25484
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2169876508
- Cited by
- 266
- References
- 49
- FWCI
- 9.60
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Moya, A., Tambutté, S., Bertucci, A., Tambutté, E., Lotto, S., Vullo, D., Supuran, C. T., Allemand, D., & Zoccola, D. (2008). Carbonic Anhydrase in the Scleractinian Coral Stylophora pistillata. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m804726200
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