Cited by 26Fungal and yeast genetics researchCellular Mechanics and InteractionsAntifungal resistance and susceptibility

Orchestrated actin nucleation by the Candida albicans polarisome complex enables filamentous growth

Open access
Xie, Ying, Loh, Zhi Yang, Xue, Jiao, Zhou, Feng, Sun, Jialin, Qiao, Zhu, Jin, Shengyang, Deng, Yinyue +5 more·2020·Journal of Biological Chemistry

Abstract

Candida albicans is a dimorphic fungus that converts from a yeast form to a hyphae form during infection. This switch requires the formation of actin cable to coordinate polarized cell growth. It's known that nucleation of this cable requires a multiprotein complex localized at the tip called the polarisome, but the mechanisms underpinning this process were unclear. Here, we found that C. albicans Aip5, a homolog of polarisome component ScAip5 in Saccharomyces cerevisiae that nucleates actin polymerization and synergizes with the formin ScBni1, regulates actin assembly and hyphae growth synergistically with other polarisome proteins Bni1, Bud6, and Spa2. The C terminus of Aip5 binds directly to G-actin, Bni1, and the C-terminal of Bud6, which form the core of the nucleation complex to polymerize F-actin. Based on insights from structural biology and molecular dynamic simulations, we propose a possible complex conformation of the actin nucleation core, which provides cooperative positioning and supports the synergistic actin nucleation activity of a tri-protein complex Bni1-Bud6-Aip5. Together with known interactions of Bni1 with Bud6 and Aip5 in S. cerevisiae , our findings unravel molecular mechanisms of C. albicans by which the tri-protein complex coordinates the actin nucleation in actin cable assembly and hyphal growth, which is likely a conserved mechanism in different filamentous fungi and yeast.

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Crossref · 10.1074/jbc.ra120.013890

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Authors
Xie, Y., Loh, Z. Y., Xue, J., Zhou, F., Sun, J., Qiao, Z., Jin, S., Deng, Y., Li, H., Wang, Y., Lu, L., Gao, Y., & Miao, Y.
Affiliations
  • Nanyang Technological University (SG)
  • Jinan University (CN)
  • Agency for Science, Technology and Research (SG)
  • Sun Yat-sen University (CN)
Corresponding author
Miao, Yansong
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2020-10
Volume
295
Issue
44
Pages
14840-14854
ISSN
0021-9258 (ISSN-L)
OpenAlex
W3080859615
Cited by
26
References
72
Citations by year
2026: 2 · 2025: 4 · 2024: 5 · 2023: 3 · 2022: 3 · 2021: 8 · 2020: 1
FWCI
1.22
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Ministry of Education - Singapore (MOE2016-T2-1-005S, MOE2017-T2-1-106, 2018-T1-001-096)
  • Nanyang Technological University (M4081533)
  • Skin Research Institute of Singapore (SIG18002)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
Keywords
Candida albicans, polarisome, actin nucleation, actin cables, hyphal growth, filamentous growth, Bni1, Spa2, formins, G-actin binding, polarized growth, dimorphic fungi, yeast-to-hypha transition
MeSH terms
Actins, Candida albicans, Fungal Proteins, Polymerization

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