Orchestrated actin nucleation by the Candida albicans polarisome complex enables filamentous growth
Open accessAbstract
Candida albicans is a dimorphic fungus that converts from a yeast form to a hyphae form during infection. This switch requires the formation of actin cable to coordinate polarized cell growth. It's known that nucleation of this cable requires a multiprotein complex localized at the tip called the polarisome, but the mechanisms underpinning this process were unclear. Here, we found that C. albicans Aip5, a homolog of polarisome component ScAip5 in Saccharomyces cerevisiae that nucleates actin polymerization and synergizes with the formin ScBni1, regulates actin assembly and hyphae growth synergistically with other polarisome proteins Bni1, Bud6, and Spa2. The C terminus of Aip5 binds directly to G-actin, Bni1, and the C-terminal of Bud6, which form the core of the nucleation complex to polymerize F-actin. Based on insights from structural biology and molecular dynamic simulations, we propose a possible complex conformation of the actin nucleation core, which provides cooperative positioning and supports the synergistic actin nucleation activity of a tri-protein complex Bni1-Bud6-Aip5. Together with known interactions of Bni1 with Bud6 and Aip5 in S. cerevisiae , our findings unravel molecular mechanisms of C. albicans by which the tri-protein complex coordinates the actin nucleation in actin cable assembly and hyphal growth, which is likely a conserved mechanism in different filamentous fungi and yeast.
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- Authors
- Xie, Y., Loh, Z. Y., Xue, J., Zhou, F., Sun, J., Qiao, Z., Jin, S., Deng, Y., Li, H., Wang, Y., Lu, L., Gao, Y., & Miao, Y.
- Affiliations
- Nanyang Technological University (SG)
- Jinan University (CN)
- Agency for Science, Technology and Research (SG)
- Sun Yat-sen University (CN)
- Corresponding author
- Miao, Yansong
- ORCID
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2020-10
- Volume
- 295
- Issue
- 44
- Pages
- 14840-14854
- ISSN
- 0021-9258 (ISSN-L)
- PMC
- PMC7606692
- OpenAlex
- W3080859615
- Cited by
- 26
- References
- 72
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 5 · 2023: 3 · 2022: 3 · 2021: 8 · 2020: 1
- FWCI
- 1.22
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education - Singapore (MOE2016-T2-1-005S, MOE2017-T2-1-106, 2018-T1-001-096)
- Nanyang Technological University (M4081533)
- Skin Research Institute of Singapore (SIG18002)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Keywords
- Candida albicans, polarisome, actin nucleation, actin cables, hyphal growth, filamentous growth, Bni1, Spa2, formins, G-actin binding, polarized growth, dimorphic fungi, yeast-to-hypha transition
- MeSH terms
- Actins, Candida albicans, Fungal Proteins, Polymerization
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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