The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
The Q Exactive HF, a Benchtop Mass Spectrometer with a Pre-filter, High-performance Quadrupole and an Ultra-high-field Orbitrap Analyzer
Open accessAbstract
The quadrupole Orbitrap mass spectrometer (Q Exactive) made a powerful proteomics instrument available in a benchtop format. It significantly boosted the number of proteins analyzable per hour and has now evolved into a proteomics analysis workhorse for many laboratories. Here we describe the Q Exactive Plus and Q Exactive HF mass spectrometers, which feature several innovations in comparison to the original Q Exactive instrument. A low-resolution pre-filter has been implemented within the injection flatapole, preventing unwanted ions from entering deep into the system, and thereby increasing its robustness. A new segmented quadrupole, with higher fidelity of isolation efficiency over a wide range of isolation windows, provides an almost 2-fold improvement of transmission at narrow isolation widths. Additionally, the Q Exactive HF has a compact Orbitrap analyzer, leading to higher field strength and almost doubling the resolution at the same transient times. With its very fast isolation and fragmentation capabilities, the instrument achieves overall cycle times of 1 s for a top 15 to 20 higher energy collisional dissociation method. We demonstrate the identification of 5000 proteins in standard 90-min gradients of tryptic digests of mammalian cell lysate, an increase of over 40% for detected peptides and over 20% for detected proteins. Additionally, we tested the instrument on peptide phosphorylation enriched samples, for which an improvement of up to 60% class I sites was observed.
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Cites
Cox, J., & Mann, M. (2008) · Nature Biotechnology · doi:10.1038/nbt.1511 · Cited by 14,855
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Universal sample preparation method for proteome analysis
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Self-citationMass spectrometry-based proteomics
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Self-citationGillet, L. C., Navarro, P., Tate, S., Röst, H., Selevsek, N., Reiter, L., Bonner, R., & Aebersold, R. (2012) · Molecular & Cellular Proteomics · doi:10.1074/mcp.o111.016717 · Cited by 2,877
Interpretation of Shotgun Proteomic Data
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Self-citationThe quantitative proteome of a human cell line
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Itzhak, D. N., Tyanova, S., Cox, J., & Borner, G. H. (2016) · eLife · doi:10.7554/elife.16950 · Cited by 649
Metabolic modulation of tumours with engineered bacteria for immunotherapy
Canale, F. P., Basso, C., Antonini, G., Perotti, M., Li, N., Sokolovska, A., Neumann, J., James, M. J., Geiger, S., Jin, W., et al. (2021) · Nature · doi:10.1038/s41586-021-04003-2 · Cited by 619
Genetic diagnosis of Mendelian disorders via RNA sequencing
Kremer, L. S., Bader, D. M., Mertes, C., Kopajtich, R., Pichler, G., Iuso, A., Haack, T. B., Graf, E., Schwarzmayr, T., Terrile, C., et al. (2017) · Nature Communications · doi:10.1038/ncomms15824 · Cited by 590
Metadata
- Authors
- Scheltema, R. A., Hauschild, J.-P., Lange, O., Hornburg, D., Denisov, E., Damoc, E., Kuehn, A., Makarov, A., & Mann, M.
- Venue
- Molecular & Cellular Proteomics
- Publisher
- Elsevier BV
- Published
- 2014-12
- Volume
- 13
- Issue
- 12
- Pages
- 3698-3708
- ISSN
- 1535-9476 (ISSN-L)
- OpenAlex
- W2152095891
- Cited by
- 323
- References
- 15
- FWCI
- 15.65
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Scheltema, R. A., Hauschild, J.-P., Lange, O., Hornburg, D., Denisov, E., Damoc, E., Kuehn, A., Makarov, A., & Mann, M. (2014). The Q Exactive HF, a Benchtop Mass Spectrometer with a Pre-filter, High-performance Quadrupole and an Ultra-high-field Orbitrap Analyzer. Molecular & Cellular Proteomics. https://doi.org/10.1074/mcp.m114.043489
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