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Antibody-based Proteomics for Human Tissue Profiling
Open accessAbstract
Here, we describe the use of antibody-based proteomics involving the generation of protein-specific antibodies to functionally explore the human proteome. The antibodies can be used for analysis of corresponding proteins in a wide range of assay platforms, including i) immunohistochemistry for detailed tissue profiling, ii) specific affinity reagents for various functional protein assays, and iii) capture ("pull-down") reagents for purification of specific proteins and their associated complexes for structural and biochemical analyses. In this review, the use of antibodies for such analysis will be discussed with focus on the possibility to create a descriptive and comprehensive protein atlas for tissue distribution and subcellular localization of human proteins in both normal and disease tissues.
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Citations
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Alternative splicing in disease and therapy
Garcia-Blanco, M. A., Baraniak, A. P., & Lasda, E. L. (2004) · Nature Biotechnology · doi:10.1038/nbt964 · Cited by 527
Cited by
A Human Protein Atlas for Normal and Cancer Tissues Based on Antibody Proteomics
Uhlén, M., Björling, E., Agaton, C., Szigyarto, C. A.-K., Amini, B., Andersen, E., Andersson, A.-C., Angelidou, P., Asplund, A., Asplund, C., et al. (2005) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m500279-mcp200 · Cited by 1,540
Interpretation of Shotgun Proteomic Data
Nesvizhskii, A. I., & Aebersold, R. (2005) · Molecular & Cellular Proteomics · doi:10.1074/mcp.r500012-mcp200 · Cited by 1,025
Ku, T., Swaney, J., Park, J.-Y., Albanese, A., Murray, E., Cho, J. H., Park, Y.-G., Mangena, V., Chen, J., & Chung, K. (2016) · Nature Biotechnology · doi:10.1038/nbt.3641 · Cited by 458
Pontén, F., Schwenk, J. M., Asplund, A., & Edqvist, P.-H. D. (2011) · Journal of Internal Medicine · doi:10.1111/j.1365-2796.2011.02427.x · Cited by 305
Proteomic analysis of formalin-fixed paraffin-embedded tissue by MALDI imaging mass spectrometry
Casadonte, R., & Caprioli, R. M. (2011) · Nature Protocols · doi:10.1038/nprot.2011.388 · Cited by 279
Metadata
- Authors
- Uhlen, M., & Ponten, F.
- Venue
- Molecular & Cellular Proteomics
- Publisher
- Elsevier BV
- Published
- 2005-04
- Volume
- 4
- Issue
- 4
- Pages
- 384-393
- ISSN
- 1535-9476 (ISSN-L)
- OpenAlex
- W2106845528
- Cited by
- 303
- References
- 1
- FWCI
- 15.76
- Type
- journal article
- Language
- en
- Open access
- hybrid
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
