The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
Proteomic analysis of formalin-fixed paraffin-embedded tissue by MALDI imaging mass spectrometry
Open accessAbstract
Archived formalin-fixed paraffin-embedded (FFPE) tissue collections represent a valuable informational resource for proteomic studies. Multiple FFPE core biopsies can be assembled in a single block to form tissue microarrays (TMAs). We describe a protocol for analyzing protein in FFPE-TMAs using matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS). The workflow incorporates an antigen retrieval step following deparaffinization, in situ trypsin digestion, matrix application and then mass spectrometry signal acquisition. The direct analysis of FFPE-TMA tissue using IMS allows direct analysis of multiple tissue samples in a single experiment without extraction and purification of proteins. The advantages of high speed and throughput, easy sample handling and excellent reproducibility make this technology a favorable approach for the proteomic analysis of clinical research cohorts with large sample numbers. For example, TMA analysis of 300 FFPE cores would typically require 6 h of total time through data acquisition, not including data analysis.
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Tissue microarrays for high-throughput molecular profiling of tumor specimens
Kononen, J., Bubendorf, L., Kallionimeni, A., Bärlund, M., Schraml, P., Leighton, S., Torhorst, J., Mihatsch, M. J., Sauter, G., & Kallionimeni, O.-P. (1998) · Nature Medicine · doi:10.1038/nm0798-844 · Cited by 4,099
Shi, S. R., Key, M. E., & Kalra, K. L. (1991) · Journal of Histochemistry & Cytochemistry · doi:10.1177/39.6.1709656 · Cited by 2,552
Going deeper than microscopy: the optical imaging frontier in biology
Ntziachristos, V. (2010) · Nature Methods · doi:10.1038/nmeth.1483 · Cited by 1,896
Analysis of Proteins and Proteomes by Mass Spectrometry
Mann, M., Hendrickson, R. C., & Pandey, A. (2001) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.70.1.437 · Cited by 1,219
Schwartz, S. A., Reyzer, M. L., & Caprioli, R. M. (2003) · Journal of Mass Spectrometry · doi:10.1002/jms.505 · Cited by 646
Self-citationEffect of formalin tissue fixation and processing on immunohistochemistry
Werner, M., Chott, A., Fabiano, A., & Battifora, H. (2000) · The American Journal of Surgical Pathology · doi:10.1097/00000478-200007000-00014 · Cited by 598
High-throughput fluorescence microscopy for systems biology
Pepperkok, R., & Ellenberg, J. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1979 · Cited by 412
Groseclose, M. R., Andersson, M., Hardesty, W. M., & Caprioli, R. M. (2007) · Journal of Mass Spectrometry · doi:10.1002/jms.1177 · Cited by 377
Self-citationIntegrating Histology and Imaging Mass Spectrometry
Chaurand, P., Schwartz, S. A., Billheimer, D., Xu, B. J., Crecelius, A., & Caprioli, R. M. (2004) · Analytical Chemistry · doi:10.1021/ac0351264 · Cited by 365
Self-citationAutomated Acoustic Matrix Deposition for MALDI Sample Preparation
Aerni, H.-R., Cornett, D. S., & Caprioli, R. M. (2006) · Analytical Chemistry · doi:10.1021/ac051534r · Cited by 344
Self-citation
Cited by
Körber, A., Anthony, I. G. M., & Heeren, R. M. A. (2025) · Analytical Chemistry · doi:10.1021/acs.analchem.4c05249 · Cited by 88
Metadata
- Authors
- Casadonte, R., & Caprioli, R. M.
- Venue
- Nature Protocols
- Published
- 2011-10-13
- Volume
- 6
- Issue
- 11
- Pages
- 1695-1709
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- OpenAlex
- W2027375922
- Cited by
- 279
- References
- 41
- FWCI
- 9.85
- Type
- journal article
- Language
- en
- Open access
- green
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