The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Multiplexed and scalable super-resolution imaging of three-dimensional protein localization in size-adjustable tissues
Open accessAbstract
The biology of multicellular organisms is coordinated across multiple size scales, from the subnanoscale of molecules to the macroscale, tissue-wide interconnectivity of cell populations. Here we introduce a method for super-resolution imaging of the multiscale organization of intact tissues. The method, called magnified analysis of the proteome (MAP), linearly expands entire organs fourfold while preserving their overall architecture and three-dimensional proteome organization. MAP is based on the observation that preventing crosslinking within and between endogenous proteins during hydrogel-tissue hybridization allows for natural expansion upon protein denaturation and dissociation. The expanded tissue preserves its protein content, its fine subcellular details, and its organ-scale intercellular connectivity. We use off-the-shelf antibodies for multiple rounds of immunolabeling and imaging of a tissue's magnified proteome, and our experiments demonstrate a success rate of 82% (100/122 antibodies tested). We show that specimen size can be reversibly modulated to image both inter-regional connections and fine synaptic architectures in the mouse brain.
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Cited by
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Highly multiplexed in situ protein imaging with signal amplification by Immuno-SABER
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Immuno-SABER enables highly multiplexed and amplified protein imaging in tissues
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Self-citationThe emerging landscape of spatial profiling technologies
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Metadata
- Authors
- Ku, T., Swaney, J., Park, J.-Y., Albanese, A., Murray, E., Cho, J. H., Park, Y.-G., Mangena, V., Chen, J., & Chung, K.
- Venue
- Nature Biotechnology
- Published
- 2016-07-25
- Volume
- 34
- Issue
- 9
- Pages
- 973-981
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W2494923364
- Cited by
- 458
- References
- 35
- FWCI
- 16.47
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Ku, T., Swaney, J., Park, J.-Y., Albanese, A., Murray, E., Cho, J. H., Park, Y.-G., Mangena, V., Chen, J., & Chung, K. (2016). Multiplexed and scalable super-resolution imaging of three-dimensional protein localization in size-adjustable tissues. Nature Biotechnology. https://doi.org/10.1038/nbt.3641
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