CoChat
Sign Up
Cited by 37

The DOI matches its Crossref record.

Modulation of the epidermal growth factor receptor by the human papillomavirus type 16 E5 protein in raft cultures of human keratinocytes

Tomakidi, Pascal, Cheng, Hao, Kohl, Annette, Komposch, Gerda, Alonso, Angel·2000·European Journal of Cell Biology
DOI 10.1078/0171-9335-00060
Open this paper in CoChat — free account

Abstract

It has been shown that the E5 protein of the human papillomavirus type 16 modulates epidermal growth factor receptor downregulation in monolayer cultures of human keratinocytes and mouse fibroblasts. We have now analysed the effect of this protein on the expression, the distribution and the activation of EGF receptors in raft cultures derived from an E5-transfected human keratinocyte cell line. The epithelia generated in these cultures were stratified and exhibited suprabasal expression of cytokeratins 1 and 10, which are known markers of early epidermal differentiation. In situ hybridization with an antisense riboprobe to the human papilloma virus type 16 E5 protein revealed a homogeneous gene expression within the entire epithelium of E5-transfected but not empty vector-transfected control cultures. Treatment of serum-starved rafts with EGF for 48 hours led to a strong decrease of suprabasal EGF receptors in control cultures, but not in rafts of E5-expressing cells. Under these conditions, no activated receptors were observed in control cultures, but activated receptors were still present in E5-raft cultures. Our results indicate that human papilloma virus type 16 E5-mediated modulation of EGF receptor expression occurs in a time- and structure-dependent manner in epithelial equivalents of human keratinocytes.

Overview

Integrity signals

Registry

Crossref · 10.1078/0171-9335-00060

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 26

  1. Auvinen, 1997, Human papillomavirus type 16 E5 protein (Review), Int. J. Oncol., 11, 1297
  2. Boelsma, 1999, Reconstruction of a human skin equivalent using a spontaneously transformed keratinocytes cell line (HaCaT), J. Invest. Dermatol., 112, 489, 10.1046/j.1523-1747.1999.00545.x · DOI
  3. Borchers, 1997, Fibroblast-directed expression and localization of 92-kDa type IV collagenase along the tumor-stroma interface in an in vitro three-dimensional model of human squamous cell carcinoma, Mol. Carcinog., 19, 258, 10.1002/(SICI)1098-2744(199708)19:4<258::AID-MC7>3.0.CO;2-8 · DOI
  4. Bosch, 1993, Expression of the histone H3 gene in benign, semi-malignant and malignant lesions of the head and neck: a reliable proliferation marker, Eur. J. Cancer, 10, 1454, 10.1016/0959-8049(93)90020-G · DOI
  5. Boukamp, 1988, Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte line, J. Cell Biol., 106, 761, 10.1083/jcb.106.3.761 · DOI · Paper page
  6. Carpenter, 1987, Receptors for epidermal growth factor and other polypeptide mitogens, Annu. Rev. Biochem., 56, 881, 10.1146/annurev.bi.56.070187.004313 · DOI
  7. Crusius, 1988, The human papillomavirus type 16 E5 protein modulates ligand-dependent activation of the EGF receptor family in the human epithelial cell line HaCaT., Exp. Cell Res., 241, 76, 10.1006/excr.1998.4024 · DOI · Paper page
  8. Crusius, 1997, Enhancement of EGF-and PMA-mediated MAP kinase activation in cells expressing the human papillomavirus type 16 E5 protein, Oncogene, 15, 1437, 10.1038/sj.onc.1201312 · DOI · Paper page
  9. Dale, 1990, New approaches and concepts in the study of differentiation of oral epithelia, Crit. Rev. Oral Biol. Med., 1, 167, 10.1177/10454411900010030201 · DOI
  10. Feghali-Assaly, 1994, Cytokeratin profile of the junctional epithelium in partially erupted teeth, J. Periodont. Res., 29, 185, 10.1111/j.1600-0765.1994.tb01212.x · DOI
  11. Galvin, 1989, The major pathways of keratinocyte differentiation defined by keratin expression: an overview, Adv. Dermatol., 4, 277
  12. Game, 1992, Progressive abrogation of TGF-b1 and EGF growth control is associated with tumour progression in ras-transfected human keratinocytes, Int. J. Cancer, 52, 461, 10.1002/ijc.2910520322 · DOI
  13. Maruo, 1992, Immunohistochemical demonstration of elevated expression of epidermal growth factor receptor in the neoplastic changes of cervical squamous epithelium, Cancer, 69, 1182, 10.1002/cncr.2820690519 · DOI
  14. Maruo, 1998, Factor regulating SCC antigen expression in squamous cell carcinoma of the uterine cervix, Tumour Biol., 19, 494, 10.1159/000030043 · DOI
  15. Mayer, 1998, Temporal and spatial expression of the E5a protein during the differentiation-dependent life cycle of human papillomavirus type 31b, Virology, 248, 208, 10.1006/viro.1998.9262 · DOI
  16. Nanney, 1984, Comparison of epidermal growth factor binding and receptor distribution in normal human epidermis and epidermal appendages, J. Invest. Dermatol., 83, 385, 10.1111/1523-1747.ep12264708 · DOI
  17. Oelze, 1995, Human papillomavirus type 16 E5 protein affects cell-cell communication in an epithelial cell line, J. Virol., 69, 4489, 10.1128/jvi.69.7.4489-4494.1995 · DOI
  18. Schoop, 1999, Epidermal organization and differentiation of HaCaT keratinocytes in organotypic cocultures with human dermal fibroblasts, J. Invest. Dermatol., 112, 343, 10.1046/j.1523-1747.1999.00524.x · DOI
  19. Sorkin, 1998, Endocytosis and intracellular sorting of receptor tyrosine kinases, Front. Biosci., 3, 729, 10.2741/A316 · DOI
  20. Stoler, 1992, Human papillomavirus type 16 and 18 gene expression in cervical neoplasias, Hum. Pathol., 23, 117, 10.1016/0046-8177(92)90232-R · DOI
  21. Straight, 1993, The E5 oncoprotein of human papillomavirus type 16 transforms fibroblasts and effects the downregulation of the epidermal growth factor receptor in keratinocytes, J. Virol., 67, 4521, 10.1128/jvi.67.8.4521-4532.1993 · DOI · Paper page
  22. straight, 1995, The E5 oncoprotein of human papillomavirus type 16 inhibits the acidification of endosomes in human keratinocytes., J. Virol., 69, 3185, 10.1128/jvi.69.5.3185-3192.1995 · DOI
  23. Syrjänen, 1996, In vitro establishment of lytic and nonproductive infection by herpes simplex virus type 1 in three-dimensional keratinocyte cultures, J. Virol., 70, 6524, 10.1128/jvi.70.9.6524-6528.1996 · DOI
  24. Tervahauta, 1994, Epidermal growth factor receptor, c-erbB-2 proto-oncogene and estrogen receptor expression in human papillomavirus lesions of the uterine cervix, Int. J. Gynecol. Pathol., 13, 230, 10.1097/00004347-199407000-00007 · DOI
  25. Ullrich, 1990, Signal transduction by receptors with tyrosine kinase activity, Cell, 61, 203, 10.1016/0092-8674(90)90801-K · DOI · Paper page
  26. Van der Geer, 1994, Receptor protein-tyrosine kinases and their signal transduction pathways, Annu. Rev. Cell Biol., 10, 251, 10.1146/annurev.cb.10.110194.001343 · DOI

Cited by

See all 37

Metadata

Authors
Tomakidi, P., Cheng, H., Kohl, A., Komposch, G., & Alonso, A.
Venue
European Journal of Cell Biology
Publisher
Elsevier BV
Published
2000-06
Volume
79
Issue
6
Pages
407-412
ISSN
0171-9335
DOI
10.1078/0171-9335-00060
Cited by
37
References
0
Language
en
Licence
Funders
Deutsche Forschungsgemeinschaft (KO/1531/1 – 2)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Modulation of the epidermal growth factor receptor by the human papillomavirus type 16 E5 protein in raft cultures of human keratinocytes. https://app.cochat.ai/papers/10.1078/0171-9335-00060
The paper
Tomakidi, P., Cheng, H., Kohl, A., Komposch, G., & Alonso, A. (2000). Modulation of the epidermal growth factor receptor by the human papillomavirus type 16 E5 protein in raft cultures of human keratinocytes. European Journal of Cell Biology. https://doi.org/10.1078/0171-9335-00060