Excretion of purine derivatives by ruminants: recycling of allantoin into the rumen via saliva and its fate in the gut
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The saliva of sheep was shown to contain significant concentrations of uric acid (16(SD 4.5) mumol/l) and allantoin (120(SD 16.4) mumol/l), sufficient to recycle purine derivatives equivalent to about 0.10 of the normal urinary excretion. When allantoin was incubated in vitro in rumen fluid, it was degraded at a rate sufficient to ensure complete destruction of recycled allantoin. In a series of experiments in which allantoin was infused into the rumen of sheep fed normally, or into the rumen or abomasum of sheep and the rumen of cattle completely nourished by intragastric infusion of volatile fatty acids and casein, no additional allantoin was recovered in the urine. These losses were probably due to the degradation of allantoin by micro-organisms associated with the digestive tract. It is concluded that all allantion and uric acid recycled to the rumen via saliva will be similarly degraded. Therefore, the use of urinary excretion of purine derivatives as an estimator of the rumen microbial biomass available to ruminants will need to be corrected for such losses.
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Urinary metabolites derived from microbial purines (allantoin and uric acid) have been used to estimate microbial protein synthesis.
“More recent attempts to develop non-invasive biomarker approaches to estimate microbial protein synthesis have used urinary metabolites derived from microbial purines (allantoin and uric acid; Chen et al., 1990).”
From Addressing global ruminant agricultural challenges through understanding the rumen microbiome: past, present, and future (2018) · cites this paper as Chen et al., 1990
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Cites
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Research works printed but not linked: 2
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Huws, S. A., Creevey, C. J., Oyama, L. B., Mizrahi, I., Denman, S. E., Popova, M., Muñoz-Tamayo, R., Forano, E., Waters, S. M., Hess, M., et al. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.02161 · Cited by 487 · see citing works
- Authors
- Chen, X. B., Hovell, F. D. D., & ØRskov, E. R.
- Venue
- British Journal Of Nutrition
- Publisher
- Cambridge University Press (CUP)
- Published
- 1990-03
- Volume
- 63
- Issue
- 2
- Pages
- 197-205
- ISSN
- 0007-1145 (ISSN-L)
- 1475-2662
- OpenAlex
- W2019856716
- Cited by
- 47
- References
- 18
- Citations by year
- 2026: 2 · 2025: 2 · 2023: 1 · 2021: 2 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 1
- FWCI
- 6.16
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- British Journal Of Nutrition (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Life Sciences › Agricultural and Biological Sciences › Soil Science › Soil Carbon and Nitrogen Dynamics
- Keywords
- purine derivatives, allantoin, uric acid, rumen, saliva, ruminants, sheep, cattle, urinary excretion, abomasum, volatile fatty acids
- MeSH terms
- Allantoin, Animals, Digestion, Enteral Nutrition, Female, Purines, Rumen, Saliva, Sheep, Uric Acid
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