Detection and serogroup determination of neisseria meningitidis in CSF by polymerase chain reaction (PCR)
Abstract
A PCR protocol for the detection and serogroup determination of Neisseria meningitidis in CSF from 85 cases of suspected meningitis was evaluated. Screening assays for both IS1106 and the ctrA gene were used to detect meningococcal DNA, and a further two assays using the siaD gene were performed to determine the serogroup. PCR results were compared with results of bacteriological culture and discrepant results resolved by analysis of clinical data and further laboratory test results. The resolved sensitivity and specificity of the PCR screening assay were 89 and 100%, and those of bacteriological culture were 37 and 100%, respectively. The siaD B/C PCR assay was able to determine a serogroup in 85% of cases positive by the PCR screening assay compared with 50% of cases where a serogroup was determined by traditional methods. PCR is a useful tool for diagnosis of meningococcal meningitis when Gram stain and culture tests are negative, a situation that may arise when antibiotic treatment has commenced prior to lumbar puncture.
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In studies assessing meningococcal PCR versus culture, sensitivity ranged from 91% to 100% with a median of 97%, and median specificity was 96% (range 91%-100%).
“Sensitivity in these studies ranged from 91% to 100% with a median of 97% and median specificity was 96% (range 91%–100%).”
From Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections (2024) · cites this paper as [64]
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Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections
Olie, S. E., Andersen, C. Ø., van de Beek, D., & Brouwer, M. C. (2024) · Clinical Microbiology Reviews · doi:10.1128/cmr.00021-24 · Cited by 38 · see citing works
“(63, 64, 173).”
- Authors
- Porritt, R. J., Mercer, J. L., & Munro, R.
- Corresponding author
- Porritt, Robert J.
- Venue
- Pathology
- Publisher
- Elsevier BV
- Published
- 2000
- Volume
- 32
- Issue
- 1
- Pages
- 42-45
- ISSN
- 0031-3025 (ISSN-L)
- OpenAlex
- W2151352005
- Cited by
- 40
- References
- 13
- Citations by year
- 2024: 1 · 2022: 1 · 2017: 1 · 2016: 1 · 2015: 2 · 2012: 3
- FWCI
- 3.02
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Pathology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- NSW Ministry of Health
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Bacterial Infections and Vaccines
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Neonatal and Maternal Infections
- Keywords
- Neisseria meningitidis, meningococcal meningitis, cerebrospinal fluid, PCR detection, bacterial culture, sensitivity and specificity
- MeSH terms
- Cerebrospinal Fluid, DNA, Bacterial, Evaluation Studies as Topic, Genes, Bacterial, Humans, Meningitis, Meningococcal, Neisseria meningitidis, Sensitivity and Specificity, Serotyping, Sialyltransferases, Polymerase Chain Reaction
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